Abstract
We have examined the spatiotemporal aspects of PRL-induced Ca2+ signals using high-speed fluo-3 confocal imaging. We found that PRL stimulated Ca2+ entry and intracellular Ca2+ mobilization. Ca2+ influx was seen as a peripheral increase in [Ca2+]i without amplification in the nucleus region. Intracellular Ca2+ mobilization was seen as a propagating intracellular calcium wave with a strong amplification in the nuclear region. The amplitude of PRL-induced Ca2+ increases would be sufficient to stimulate cell proliferation. Furthermore, PRL induced an increase in [3H]thymidine incorporation. These data suggest that PRL would be able to induce mitogenesis through a Ca2+-dependent pathway.
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Ducret, T., Sorin, B., Vacher, A.M. et al. Spatiotemporal Aspects of Prolactin-Induced Calcium Signaling. Journal of Fluorescence 12, 253–258 (2002). https://doi.org/10.1023/A:1016877204552
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DOI: https://doi.org/10.1023/A:1016877204552