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The Medical Use of Rescaling Procedures in Optical Biopsy and Optical Molecular Imaging

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Abstract

Laser-induced autofluorescences show a strong intensity distortion for endogenous NADH in the UV and synthesized markers in the NIR range because of tissue optics. Rescaling taking into account bio-optical methods results in the chromophore profile in the observed tissue region. For the in vivo tests an experimental NIR imager was used. NIR fluorescence of the entire body of small animals can be imaged. For first experiments an undifferentiated superficial tumor of mouse thigh was used. Corrections that are due to tissue optics must take care of a more strongly scattering of the light in the NIR range in comparison to the UV fluorescence such as in optical biopsy. For example, the diameter of the fluorescent volume is apparently larger for the same reason. Therefore, the established rescaling from the UV adapted to the NIR range is important for the interpretation fluorescence pictures in biomedicine.

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Authors and Affiliations

  1. FU Berlin, Institut für Medizinische Physik/Lasermedizin, Fabeckstr. 60-62, 14195, Berlin, Germany

    O. Minet & J. Beuthan

  2. Laser- und Medizin- Technologie GmbH Berlin, Fabeckstr. 60-62, 14195, Berlin, Germany

    O. Minet & C. Mahnke

  3. Schering AG Berlin, Müllerstrasse 178, 13342, Berlin, Germany

    K. Licha

Authors
  1. O. Minet
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  2. J. Beuthan
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  3. K. Licha
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  4. C. Mahnke
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Correspondence to O. Minet.

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Minet, O., Beuthan, J., Licha, K. et al. The Medical Use of Rescaling Procedures in Optical Biopsy and Optical Molecular Imaging. Journal of Fluorescence 12, 201–204 (2002). https://doi.org/10.1023/A:1016856600009

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  • DOI: https://doi.org/10.1023/A:1016856600009

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