Abstract
Preimplantation genetic diagnosis (PGD) is diagnostic tool to avoid inheritance of genetic disease by transferring unaffected embryos. Recently, PCR and FISH have been mainly applied to the diagnosis of single gene disorders and chromosomal abnormalities, respectively. Since with PGD, only a few cells are available for genetic tests, both gene and chromosomes analysis have to be obtained from the same, limited material. Cell recycling makes it possible to obtain the information on genes as well as chromosomes from the same cells. Therefore cell recycling is an acceptable strategy where in PGD targets large proportions of embryos severe chromosomal abnormalities. The responsible genes of the X-linked disorder and numerical abnormalities of sex chromosomes should be analyzed simultaneously. Gender information is definitely useful because only male affected embryos should be avoided for transfer.
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REFERENCES
Munne S, Alikani M, Tomkin G, Grifo J, Cohen J: Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities. Fertil Steril 1995;64:382–391
Sasabe Y, Katayama KP, Nishimura T, Takahashi A, Asakura H, Winchester-Peden K, Wise L, Abe Y, Kubo H, Hirakawa S: Preimplantation diagnosis by fluorescence in situ hybridization using 13, 16, 18, 21, 22, X and Y chromosome probes. J Assist Reprod Genet 1999;16:90–95
Thornhill AR, Monk M: Cell recycling of a single human cell for preimplantation diagnosis of X-linked disease and dual sex determination. Mol Hum Reprod 1996;2:285–289
Rechitsky S, Freidine M, Verlinsky Y, Strom CM: Allele dropout in sequential PCR and FISH analysis of single cells (cell recycling). J Assist Reprod Genet 1996;13:115–124
Munne S, Magli C, Cohen J, Morton P, Sadowy S, Gianaroli L, Tucker M, Marquez C, Sable D, Ferraretti AP, Massey JB, Scott R: Positive outcome after preimplantation diagnosis of aneuploidy in human embryos. HumReprod 1999;14:2191–2199
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Kubo, H., Sasabe, Y. & Nishimura, T. Analysis of Sex Chromosomes in Preimplantation Genetic Diagnosis for X-Chromosome-Linked Disorders. J Assist Reprod Genet 19, 447–449 (2002). https://doi.org/10.1023/A:1016828110429
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DOI: https://doi.org/10.1023/A:1016828110429