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Visualization of Intracellular CA2+ Dynamics with Simultaneous 2-Photon Excited Fluorescence and Third Harmonic Generation Microscope

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Abstract

We show that the simultaneous coupling of two laser scanning non-linear microscopies (2-photon excited fluorescence and third harmonic generation microscopy) give functional and morphological information for improving our understanding on intracellular calcium flows.

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Authors and Affiliations

  1. Centre de Physique Moléculaire et ondes Hertziennes, CNRS UMR 5798 351, Crs de la libération, 33405, Talence Cedex, France

    S. Rivet, L. Canioni & L. Sarger

  2. Laboratoire des Propriétés Optiques des matériaux et applications, CNRS UMR 6136, 4, Bd Lavoisier, BP 2018,49016, Angers Cedex, France

    R. Barille

  3. Laboratoire de Physiologie et Physiopathologie de la Signalisation Cellulaire, CNRS UMR 5543 146, rue Léo Saignat, 33076, Bordeaux Cedex, France

    Pierre Vacher & Thomas Ducret

Authors
  1. S. Rivet
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  2. L. Canioni
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  3. L. Sarger
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  4. R. Barille
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  5. Pierre Vacher
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  6. Thomas Ducret
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Correspondence to R. Barille.

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Rivet, S., Canioni, L., Sarger, L. et al. Visualization of Intracellular CA2+ Dynamics with Simultaneous 2-Photon Excited Fluorescence and Third Harmonic Generation Microscope. Journal of Fluorescence 12, 197–199 (2002). https://doi.org/10.1023/A:1016804615939

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  • DOI: https://doi.org/10.1023/A:1016804615939

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