Skip to main content
SpringerLink
Log in

On the Possibility of Glucose Sensing Using Boronic Acid and a Luminescent Ruthenium Metal-Ligand Complex

  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

We describe a new approach to optical sensing of glucose based on the competitive interactions between a ruthenium metal ligand complex, a boronic acid derivative and glucose. The metal-ligand complex [Ru(2,2′-bipyridine)2(5,6-dihydroxy-1,10-phenanthroline)](PF6)2 at pH 8 forms a reversible complex with 2-toluylboronic acid or 2-methoxyphenyl boronic acid. Complexation is accompanied by a several-fold increase in the luminescent intensity of the ruthenium complex. Addition of glucose results in decreased luminescent intensity, which appears to be the result of decreased binding between the metal-ligand complex and the boronic acid. Ruthenium metal-ligand complexes are convenient for optical sensing because their long luminescent decay times allow lifetime-based sensing with simple instrumentation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. National Institutes of Diabetes and Digestive and Kidney Disorders (June 1993). The Diabetes Control and Complications Trial, NIH Publication.

  2. R. J. McNichols and G. L. Cote (1999) J. Biomed. Optics.(in press.)

  3. J. S. Schultz and G. Sims (1979) Biotech. Bioeng. Symp. 9, 65–71.

    Google Scholar 

  4. J. Schultz, S. Mansouri, and I. J. Goldstein (1982) Diabetes Care Bioeng. 5(3), 245–253.

    Google Scholar 

  5. D. Meadows and J. S. Schultz, (1988) Talanta 35(2), 145–150.

    Google Scholar 

  6. J. R. Lacowicz and B. P. Maliwal (1993) Anal. Chim. Acta 271, 155–164.

    Google Scholar 

  7. L. Tolosa, H. Szmacinski, G. Rao, and J. R. Lacowicz (1997) Anal. Biochem. 250, 102–108.

    Google Scholar 

  8. L. Tolosa, H. Malak, G. Rao, and J. R. Lacowicz (1997) Sensors and Actuators 45, 93–99.

    Google Scholar 

  9. J. S. Marvin, H. W. Hellinga (1998) J. Am. Chem. Soc. 120, 120 7–11.

    Google Scholar 

  10. C. L. Careaga, J. Sutherland, J. Sabeti, and J. J. Falke (1995) Biochemistry 34, 3048–3055.

    Google Scholar 

  11. G. Gilardi, L. Q. Zhou, L. Hibbert, and A. E. G. Cass (1994) Anal. Chem. 66, 3840–3847.

    Google Scholar 

  12. T. D. James, K. R. A. S. Sandanayake, and S. Shinkai (1994) J. Chem. Soc., Chem. Commun. pp. 477–478.

  13. T. D. James, K. R. A. S. Sandanayake, and S. Shinkai (1995) Nature 74, 345–347.

    Google Scholar 

  14. K. Kataoka, I. Hisamitsu, N. Sayama, T. Okano, and Y. Sakurai (1995) J. Biochem. 117, 1145–1147.

    Google Scholar 

  15. M. Takeshita, K. Uchida, and M. Irie (1996) Chem. Commun. pp. 1807–1808.

  16. M. Yamamoto, M. Takeuchi, and S. Shinkai (1998) Tetrahedron 54, 3125–3140.

    Google Scholar 

  17. K. R. A. S. Sandanayake, T. D. James, and S. Shinkai (1996) Pure Appl. Chem. 68, 1207–1212.

    Google Scholar 

  18. C. R. Cooper and T. D. James (1999) SPIE Proceedings in R. B. Thompson and J. R. Lakowicz (Eds.) Advances in Fluorescence Sensing Technology. (In press.)

  19. J. A. Tamada, N. J. V. Bohannon, and R. O. Pots (1995) Nature Med. 1, 1198–1201.

    Google Scholar 

  20. P. Glikfeld, R. S. Hinz, and R. H. Guy (1989) Pharm. Res. 6(11), 989–990.

    Google Scholar 

  21. J. S. Nelson, J. L. McCullough, T. C. Glenn, W. H. Wright, L. H. Liaw, and S. L. Jacques (1991) J. Invest. Dermatol. 97, 874–879.

    Google Scholar 

  22. S. L. Jacques, D. J. McAuliffe, I. H. Blank, and J. A. Parrish, (1987) J. Invest. Dermatol. 88, 88–93.

    Google Scholar 

  23. H. Malak, I. Gryczynski, J. R. Lakowicz, G. J. Meyer, and F. N. Castellano (1997) J. Fluoresc. 7(2), 107–112.

    Google Scholar 

  24. X-Q. Guo, F. N. Castellano, L. Li, and J. R. Lakowicz (1998) Anal. Chem. 70(3), 632–637.

    Google Scholar 

  25. H. Szmacinski, F. N. Castellano, E. Terpetschnig, J. D. Dattelbaum, J. R. Lakowicz, and G. J. Meyer, (1998) Biochim. Biophys. Acta. 1383, 151–159.

    Google Scholar 

  26. F. N. Castellano, J. D. Dattelbaum, and J. R. Lakowicz (1998) Anal. Biochem. 255, 165–170.

    Google Scholar 

  27. L. Li, F. N. Castellano, I. Gryczynski, and J. R. Lakowicz (1999) Chem. Phys. Lipids. (In press.)

  28. W. Paw and Eisenberg (1997) Inorg. Chem. 36, 2287–2293.

    Google Scholar 

  29. J. Sipior, G. M. Carter, J. R. Lakowicz, and G. Rao (1996) Rev. Sci. Instrum. 67(11), 3795–3798.

    Google Scholar 

  30. J. Sipior, G. Carter, and J. R. Lakowicz (1997) Rev. Sci. Instrum. 68, 2670–2666.

    Google Scholar 

  31. S. Fantini, M. A. Franceschini, J. B. Fishkin, B. Barbieri, and E. Gratton, (1994) Appl. Optics 33(22), 5204–5213.

    Google Scholar 

  32. H. Szmacinski and J. R. Lakowicz (1994) In J. R. Lakowicz (Ed) Topics in Fluorescence Spectroscopy, Vol. 4, Probe Design and Chemical Sensing, Plenum Press, New York, pp. 295–334.

    Google Scholar 

  33. L. Tolosa, I. Gryczynski, L. R. Eichhorn, J. D. Dattelbaum, J. D. Castellano, G. Rao, and J. R. Lakowicz (1999) Anal. Biochem. 267, 114–120.

    Google Scholar 

  34. J. R. Lakowicz, F. N. Castellano, J. D. Dattelbaum, L. Tolosa, G. Rao, and J. R. Lakowicz (1998) Anal. Chem. 70(24), 5115–5121.

    Google Scholar 

  35. J. R. Lakowicz, J. D. Dattelbaum, and I. Gryczynski (1999) Sensors and Actuators. (Submitted for publication.)

  36. J. R. Lakowicz, I. Gryczynski, Z. Gryczynski, L. Tolosa, J. D. Dattelbaum, and G. Rao (1998) Appl. Spectroscopy 9, 1149–1157

    Google Scholar 

  37. J. R. Lakowicz, I. Gryczynski, Z. Gryczynski, and J. D. Dattelbaum (1999) Anal. Biochem. 267, 397–405.

    Google Scholar 

  38. I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz (1999) Anal. Chem., 71, 1241–1251.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland, 21201, USA

    Zakir Murtaza, Leah Tolosa, Peter Harms & Joseph R. Lakowicz

Authors
  1. Zakir Murtaza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leah Tolosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Harms
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joseph R. Lakowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joseph R. Lakowicz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Murtaza, Z., Tolosa, L., Harms, P. et al. On the Possibility of Glucose Sensing Using Boronic Acid and a Luminescent Ruthenium Metal-Ligand Complex. Journal of Fluorescence 12, 187–192 (2002). https://doi.org/10.1023/A:1016800515030

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016800515030

Search

Navigation