Abstract
The morphology of female reproductive organs and anatomy, ultrastructure, and ultrasculpture of seed integuments were studied in more than 150 species of Podocarpales, Cephalotaxales, and Taxales. The main trends of morphological-evolutionary transformations of female fructifications were established in three studied groups of gymnosperms. The taxonomic weight of structural features of the female reproductive organs of Podocarpales, Cephalotaxales, and Taxales was determined. Seeds of 20 species of these orders were investigated using amino acid and Western blot analysis. The index of remoteness from the least specialized hypothetical taxon and degree of difference between taxa were calculated from data on amino acid composition of seeds and embryos. The differences in the degree of similarity between Podocarpales and other groups of gymnosperms were demonstrated. An original concept was proposed for phylogenetic relations between Podocarpales, Cephalotaxales, and Taxales.
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REFERENCES
Bergner, J. andJensen, U., Phytoserological Contribution to the Systematic Placement of the Thyphales, Nord. J. Bot., 1989, vol. 8, no. 5, pp. 447-456.
Bobrov, A.V., Correlations of Seed Structure with Ways of Dissemination in Representatives of the Order Podocarpales (Pinophyta), “Problemy reproduktivnoi biologii rastenii” (Abstracts of Papers at the Conference “Problems of reproductive Plant Biology”), Perm': Permsk. Gos. Univ., 1996a, pp. 60-62.
Bobrov, A.V., Bitegmal Seeds of Representatives of the Orders Podocarpales, Cephalotaxales, and Taxales, Materialy IX Moskovskogo soveshchaniya po filogenii rastenii (Materials of the 9th Moscow Conference of Plant Phylogeny), Moscow: Mosk. Gos. Univ., 1996b, pp. 23-26.
Bobrov, A.V., Seed Structure in Representatives of the Genus Torreya Am. With Reference to Its Taxonomic Status, Tezisy dokladov VI molodezhnoi konferentsii botanikov (Abstracts of Papers at the 6th Conference of Young Botanists), St. Petersburg: Bot. In-t Ross. Akad. Nauk, 1997a, p. 42.
Bobrov, A.V., Taxonomic Status and Phylogeny of the Genus Amentotaxus Pilg. (Amentotaxaceae Kudo et Yamamoto) According to the Structural Features of Female Reproductive Organs, Tezisy dokladov Mezhdunarodnoi conferentsii po anatomii I morfologii rastenii (Abstracts of Papers at the International Conference on Plant Anatomy and Morphology), St. Petersburg: Diada, 1997b, pp. 19-20.
Bobrov, A.V., Morphology, Anatomy, and Ultrastructure of Seeds in the Genus Cephalotaxus Siebold et Zucc. (Cephalotaxaceae Neger) with Reference to the Genus System and Phylogeny, Tezisy dokladov Mezhdunarodnoi conferentsii po anatomii I morfologii rastenii (Abstracts of Papers at the International Conference on Plant Anatomy and Morphology), St. Petersburg: Diada, 1997c, pp. 18-19.
Bobrov, A.V.F.Ch., System und Phylogenie der Ordnung Taxales nach den Angaben der Samenstruktur, Scripta Bot. Belgica, 1997d, vol. 15, p. 29.
Bobrov, A.V., Taxonomic Status of the Genus Saxe-Gothaea Lindl. (Podocarpaceae s.l.) According to the Structural Features of Female Reproductive Organs, Tezisy dokladov II (X) s'ezda RBO (Abstracts of Papers at the II (X) Congress of the Russian Botanical Society), St. Petersburg: Diada, 1998, vol. 1, pp. 10-11.
Bobrov, A.V., Taxonomy of the Coniferales: Critical Analysis of Traditional Views, Tezisy dokladov Soveshchaniya po problemam introduktsii khvoinykh rastenii v Rossii (Abstracts of Papers at the Conference on Problems of the Introduction of Coniferous Plants in Russia), Sochi: Subtrop, Bot. Sad Kubani, 1999a, p. 3-7.
Bobrov, A.V.F.Ch., Female Cone Structure in Coniferae, Abstr. XVI Int. Botanical Congress, St. Louis: Missouri Bot. Gard., 1999b, p. 342.
Bobrov, A.V., System, Phylogeny, and History of Dissemination of the Genus Taxus L. (Taxaceae S.F.Gray) According to the Structural Features of Female Reproductive Organs, Problemy filogenii izbrannykh grupp vysshikh rastenii (Problems of Phylogeny of Selected Groups of Higher Plants), 2001, no. 1 (in press).
Bobrov, A.V. andKostrikin, D.S., System of the Genus Dacrydium Soland. Ex Lamb s.l. (Podocarpaceae s.l.) According to the Structural Features of Female Reproductive Organs, Tezisy dokladov II (X) s'ezda RBO (Abstracts of Papers at the II (X) Congress of the Russian Botanical Society), St. Petersburg: Diada, 1998, vol. 1, pp. 11-12.
Bobrov, A.V.F.Ch. andKostrikin, D.S., Systematic Position of Microcachrys tetragona (Hook.) Hook. fil. (Podocarpaceae (Dumort.) Endl. s. 1.) Based on Seed Coat Structure, Zusammenf. Vort. 14. Symposium Biodiversitat und Evolutionsbiologie, Jena: Inst. Spez. Bot., 1999, p. 19.
Bobrov, A.V.F.Ch. andKostrikin, D.S., Systematic Position of the Genus Retrophyllum C.N.Page (Podocarpaceae (Dumort) Endl. S.l.) and Phylogeny of the Family Nageiaceae Fu s.l., Problemy filogenii izbrannykh grupp vysshikh rastenii (Problems of Phylogeny of Selected Groups of Higher Plants), 2001, no. 1 (in press).
Bobrov, A.V. andMelikian, A.P., Specific Structural Features of the Seed Coat and Their Possible Use in Taxonomy of the Family Podocarpaceae Endlicher 1847 s.l., Byull. Mosk. Obva Ispyt. Prirody. Otd. Biol., 1998a, vol. 103, no. 1, pp. 56-62.
Bobrov, A.V. andMelikian, A.P., Systematic Position of the Genus Parasitaxus de Lauben (Podocarpaceae s.l.) According to the Seed Structure, Tezisy dokladov II (X) s'ezda RBO (Abstracts of Papers at the II (X) Congress of the Russian Botanical Society), St. Petersburg: Diada, 1998, vol. 1, p. 12.
Bobrov A.V.F.Ch. andMelikian, A.P., Systematics of Coniferae, Abstr. XVI Intern. Botanical Congress, St. Louis: Missouri Bot. Gard., 1999, p. 340.
Bobrov, A.V.F.Ch. andRomanov, M.S., Seed Coat Structure and Systematic Relationships of Sundacarpus amara (Blume) C.N. Page (Podocarpaceae (Dumort.) Endl. s. 1.), Zusammenf. Vort. 14. Symposium Biodiversitat und Evolutionsbiologie, Jena: Inst. Spez. Bot., 1999, p. 20.
Bobrov, A.V.F.Ch. andZhurov, F.D., Seed Structure and Systematic Position of Saxe-Gothaea conspicna Lindl. (Podocarpaceae (Dumort.) Endl. s. 1.), Zusammenf. Vort. 14. Symposium Biodiversitat und Evolutionsbiologie, Jena: Inst. Spez. Bot., 1999, p. 21.
Bobrov, A.V.F.Ch.,Melikian, A.P., andYembaturova, E.Y., Seed Morphology, Anatomy and Ultrastructure of Phyllocladus L.C. et A. Rich. ex Mirb. (Phyllocladaceae (Pilg.) Bessy) in Connection with Generic System and Phylogeny, Ann. Bot., 1999, vol. 83, pp. 601-618.
Bobrov, A.V.F.Ch.,Melikian, A.P., andRomanov, M.S., Seed Morphology, Anatomy and Ultrastructure of Austrotaxus spicata Compton (Taxaceae S. F. Gray) with Relation to Its Systematic Position, Bot. J. Linn. Soc., 2001 (in press).
Crane, P.R., Phylogenetic Analysis of Seed Plants and the Origin of Angiosperms, Ann. Missouri Bot. Gard., 1985, vol. 72, no. 4, pp. 716-793.
Crane, P.R., Major Clades and Relationships in the “Higher” Gymnosperms, Origin and Evolution of Gymnosperms, Beck, C.B., Ed., New York: Columbia Univ., 1988, pp. 218-272.
Fu, D.-Z., Nageiaceae-a New Gymnosperm Family, Acta Phytotax.Sin., 1992, vol. 30, no. 6, pp. 515-528.
Gaussen, H., Les Gymnospermes actuelles et fossiles. Les Podocarpines, Trav. Lab. For. Toul. T. II, 1 sect., 1974, vol. 1, pp. 1-31, 1-208.
Gavrilyuk, I.P.,Gubareva, N.K., andKonarev, V.G., Isolation and Fractionation of Proteins Used in Genomic Analysis of Cultured Plants, Trudy Prikl. Botan. Gen. Sel. VIR, 1973, vol. 52, no. 1, pp. 157-164.
Holmes, W.B.K., New Corystosperm Ovulate Fructifications from the Middle Triassic of Eastern Australia, Alcheringa, 1987, vol. 11, no. 2, pp. 163-173.
Kalistratova, O.A.,Aref'eva, L.P., andMiroshnichenko,E.Ya., Variation of the Fractional and Amino Acid Composition of Seed Proteins in Biotypes Poa pratensis, Byull Glavn. Bot. Sada, 1979, no. 114, pp. 42-46.
Kudo, Y. andYamamoto, Y., Amentotaxaceae, Materials on Flora of Formosa, Kudo, Y., Ed., 1931, vol. 3, no. 1, pp. 110-112.
Lacey, W.C., Further Observations on the Molteno Flora of Rhodesia, Arnoldia, 1976, vol. 7, no. 1, pp. 1-14.
Laemmli, U.K., Cleavage of Structure Proteins during the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680-685.
de Laubenfels, D.J., A Taxonomic Revision of the Genus Podocarpus, Nature, 1985, vol. 30, no. 2, pp. 251-278.
de Laubenfels, D.J., Revision of Coniferales, Ser. 1. Seed Plants. Flora Malesiana, 1988, vol. 10, no. 3, pp. 337-453.
Meien, S.V., Osnovy paleobotaniki (Foundations of Paleobotany), Moscow: Nedra, 1987.
Melikian, A.P. andBobrov, A.V., Systematic Position of the Genus Acmopyle Pilg. (Podocarpaceae Endlicher s.l.) According to Seed Comparative Morphology, Anatomy, and Ultrastructure, Tezisy dokladov Mezhdunarodnoi conferentsii po anatomii I morfologii rastenii (Abstracts of Papers at the International Conference on Plant Anatomy and Morphology), St. Petersburg: Diada, 1997a, pp. 92-93.
Melikian, A.P. andBobrov, A.V., On Structure of External Seed Coats, Epimatium and Arillus, in Representatives of the Family Podocarpaceae Endlicher 1847 s.l., Byull. Mosk. Obva Ispyt. Prirody. Otd. Biol., 1997b, vol. 102, no. 5, pp. 46-53.
Melikian, A.P. and Bobrov, A.V.F.Ch., Die Abstammung von supraintegumentalen Samendecken-des Epimatiums und Samenmantels (“Arillus”)-bei den Vertretern der Ordnungen Taxales und Podocarpales, Scripta Bot. Belgica, 1997c, vol. 15, p. 111.
Melikian, A.P. andBobrov, A.V., On Comparative-Anatomical and Phylogenetic Significance of Chambers in the Seed Coat in Some Archaic Representatives of Seed Plants, Tezisy dokladov II (X) s'ezda RBO (Abstracts of Papers at the II (X) Congress of the Russian Botanical Society), St. Petersburg: Diada, 1998, vol. 1, p. 54.
Melikian, A.P. andBobrov, A.V., Morphology of Reproductive Organs and Experience of Development of the Phylogenetic System for the Orders Podocarpales, Cephalotaxales, and Taxales, Bot. Zh., 2000, vol. 85, no. 7, pp. 50-68.
Miflin, B.J.,Wallsgrove, R.M., andLea, P.J., Glutamine Metabolism in Higher Plants, Curr. Topic. Cell Regul., 1981, vol. 20, pp. 1-43.
Nakai, T., Indigenous Species of Conifers and Taxads of Korea and Manchuria and Their Distribution. 1, Tyôsen Sanrin Kayhô, 1938, vol. 158, no. 1, pp. 1-29.
Novozhilova, O.A. andSemikhov, V.F., Amino Acid Composition of Prote in Fractions of Seeds of Secale cereale L. and Bromopsis riparia (Rhem.) Holub, Prikl. Biokhim. Mikrobiol., 1985, vol. 21, pp. 671-676.
Osborn, J.M. andTaylor, T.N., Pollen Morphology and Ultrastructure of the Corystospermales: Permineralized in situ Grains from the Triassic of Antarctica, Rev. Palaeobot. Palynol., 1993, vol. 79, no. 2/3, pp. 205-219.
Page, C.N., Coniferophytina, The Families and Genera of Vascular Plants, Kubitzky, K., Ed., Berlin: Springer, 1990, vol. 1, pp. 281-361.
Petriella, B., Sistematica y vinculaciones de las Coryctospermaceae H. Thomas, Ameghiniana, 1981, vol. 18, no. 2, pp. 221-234.
Prozina, M.N., Botanicheskaya mikrotekhnika (Botanical Microtechnique), Moscow: Vyssh. Shk., 1960.
Retallack, G.J. andDilcher, D.L., Reconstructions of Selected Seed Ferns, Ann. Missouri Bot. Gard., 1988, vol. 75, no. 5, pp. 1010-1057.
Semikhov, V.F., A Study of Amino Acid and Fractional Composition of Seed Proteins in the Tribe Andropogoneae Dum. (Gramineae), Byull. Mosk Ob-va Ispyt. Prirody. Otd. Biol., 1986, vol. 91, no. 4, pp. 64-72.
Semikhov, V.F., The Concept of Amino Acid Composition of Seeds of a Hypothetical Ancestor of Poaceae and Its Use for Taxonomy of This Family, Botan. Zh., 1988, vol. 73, no. 9, pp. 1225-1234.
Semikhov, V.F., Evolution of the Seed Protein Complex and Estimate of Evolutionary Progress of Plant Taxa, Byull. Mosk Ob-va Ispyt. Prirody. Otd. Biol., 1989, vol. 94, no. 6, pp. 9-19.
Semikhov, V.F., Chemotaxonomy and Its Position in Plant Systematics, Zh. Obshch. Biol., 1991, vol. 52, no. 5, pp. 27-37.
Semikhov, V.F., On Problems of Taxonomy of Seed Plants, Tezisy dokladov II (X) s'ezda RBO (Abstracts of Papers at the II (X) Congress of the Russian Botanical Society), St. Petersburg: Diada, 1998, vol. 2, p. 150.
Semikhov, V.F., Substantiation of Transition to Integrative Plant Taxonomy, Materialy X Moskovskogo soveshchaniya po filogenii rastenii (Materials of the 10th Moscow Conference of Plant Phylogeny), Moscow: Mosk. Ob-vo Ispyt. Prirody, 1999, pp. 151-154.
Semikhov, V.F. andNovozhilova, O.A., Taxonomic Significance of the Seed Amino Acid Composition, Botan. Zh., 1982, vol. 67, no. 9, pp. 1207-1215.
Semikhov, V.F.,Temnikova, A.A., andKalistratova, O.A., Unknown Nonproteinogenic Amino Acid in the Goumi Seeds, Byull. Glavn. Bot. Sada, 1979, no. 112, pp. 31-34.
Semikhov, V.F.,Kalistratova, O.A., andStroev, V.S., Variability of Amino Acid Composition of Seeds in Two Wheat-Grass Species, Byull. Glavn. Bot. Sada, 1980, no. 116, pp. 51-55.
Semikhov, V.F.,Novozhilova, O.A., andAref'eva, L.P., Variability of Amino Acid Composition of Seeds and Prolamine Fraction with Reference to Their Use in Plant Taxonomy, Byull. Mosk Ob-va Ispyt. Prirody. Otd. Biol., 1982, vol. 87, no. 1, pp. 68-78.
Semikhov, V.F.,Novozhilova, O.A.,Prusakov, A.N., andAref'eva, L.P., Comparative-Biochemical Studies of the Embryo and Endosperm in Seed Plants, Izv. Ross. Akad. Nauk. Ser. Biol., 1997, no. 4, pp. 425-433.
Semikhov, V.F.,Novozhilova, O.A., andAref'eva, L.P., Main Trends of Changes in Amino Acid Composition of Monocotyledonous Seeds during Evolution, Izv. Ross. Akad. Nauk. Ser. Biol., 1998a, no. 4, pp. 483-496.
Semikhov, V.F.,Timoshchenko, A.S.,Aref'eva, L.P., andNovozhilova, O.A., Variability of Amino Acid of Seeds in Representatives of the Tribe Triticeae (Poaceae), Byull. Glavn. Bot. Sada, 1998b, no. 176, pp. 132-140.
Semikhov, V.F.,Aref'eva, L.P.,Novozhilova, O.A., et al., Changes in Amino Acid Composition of Dicotyledonous Seeds during Evolution, Izv. Ross. Akad. Nauk. Ser. Biol., 2000, no. 1, pp. 39-50.
Sozonova, L.I.,Semikhov, V.F., andEliseev, I.P., Amino Acid Composition of Seeds in Representatives of the Family Elaeagnaceae, Byull. Glavn. Bot. Sada, 1985, no. 135, pp. 44-47.
Takhtadzhyan, A.L., Vysshie rasteniya (Higher Plants), Moscow: Akad. Nauk SSSR, 1956.
Takhtadzhyan, A.L., Sistema magnolifitov (System of Magnoliphytes), Leningrad: Nauka, 1970.
Taylor, T.N. andArchangelsky, S., The Cretaceous Pteridosperms Ruflorinia and Ktalenia and Implications on Cupule and Carpel Evolution, Amer. J. Bot., 1985, vol. 72, no. 12, pp. 1842-1853.
Taylor, T.N.,Dell Fueyo, G.M., andTaylor, E.L., Permineralized Seed Fern Cupules from the Triassic of Antarctica: Implications for Cupule and Carpel Evolution, Amer. J. Bot., 1994, vol. 81, no. 6, pp. 666-677.
Towbin, H.,Staehelin, T., andGordon, A., Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications, Proc. Nat. Acad. Sci. USA, 1979, vol. 76, pp. 4350-4354.
Townrow, J.A., A New Member of Corystospermaceae Thomas, Ann. Bot., n. s., 1965. Vol. 29, no. 72, pp. 495-511.
Yao, X.,Taylor, T.N., andTaylor, E.L., The Corystosperm Pollen Organ Pteruchus from the Triassic of Antarctica, Amer. J. Bot., 1995, vol. 82, no. 4, pp. 535-546.
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Semikhov, V.F., Aref'eva, L.P., Novozhilova, O.A. et al. Systematic Relationships of Podocarpales, Cephalotaxales, and Taxales Based on Comparative Seed Anatomy and Biochemistry Data. Biology Bulletin 28, 459–470 (2001). https://doi.org/10.1023/A:1016788009479
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DOI: https://doi.org/10.1023/A:1016788009479