Abstract
We studied the influence of the duration of the joint incubation of cattle oocytes and spermatozoa (18 versus 1 h) on fertilization, cleavage, and embryonic development in vitrountil the blastocyst stage. Spermatozoa of a bull of the Britanofrizskaya breed were used in the experiments. It was shown in the first experimental series that after a long-term incubation with the spermatozoa, the percentage of penetrated eggs increased 71.7 and 56.0% (p< 0.05) after 18-hour and 1-hour incubation, respectively. However, no differences were found in the number of normally fertilized eggs: 46.5 and 39.0%, respectively. In the second experimental series, no significant differences were found in either the number of cleaving embryos (41.2 and 32.2%, respectively) or the capacity of cleaving embryos to develop in vitrountil the blastocyst stage (21.7 and 15.8%, respectively). Thus, reduction in the time of the joint incubation of cattle gametes upon in vitrofertilization to 1 h did not reduce the number of normally fertilized eggs and did not affect their capacity for subsequent in vitrodevelopment.
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REFERENCES
Aitken, J.R., A Free Radical Theory of Male Infertility, Reprod. Fertil. Devel., 1994, vol. 6, pp. 19–24.
Anderson, G.B., Advances in Large Mammalian Embryo Culture, Methods in Mammalian Reproduction Daniel, J.C., Ed., New York: Academic, 1978, pp. 273–284.
Austin, C.R., The Egg, Reproduction in Mammals. Vol. 1. Germ Cells and Fertilization, Austin, C. and Shert, R., Eds., London, 1982, pp. 46–62.
Bavister, B.D., Leibfried, M.L., and Lieberman, G., Development of Preimplantation Embryos of the Golden Hamster in a Defined Culture Medium, Biol. Reprod., 1983, vol. 28, pp. 235–247.
Coskun, S., Roca, G.L., Hollanders, J., and Jaroudi, K., Effects of Reducing Insemination Time in Human IVF and Embryo Development Using Sibling Oocytes, Hum. Reprod., 1997, vol. 12, abstract book 1, p. 112.
Coy, P., Martinez, E., Ruiz, S., et al., In vitro Fertilization of Pig Oocytes after Different Coincubation Intervals, Theriogenology, 1993, vol. 39, no. 5, pp. 1201–1208.
Cox, J.F., Hormazabal, J., and Maria, A.S., Effect of the Cumulus on in vitro Fertilization of Bovine Matured Oocytes, Theriogenology, 1993, vol. 40, no. 6, pp. 1259–1267.
Dandekar, P.V. and Glass, R.H., Development of Mouse Embryos in vitro Is Affected by Strain and Culture Medium, Gamete Res., 1987, vol. 17, no. 2, pp. 279–285.
Du, Z.F. and Wales, R.G., Some Effects of Genotype and Composition of the Culture Medium on the Development of Mouse Zygotes in vitro, Reprod. Fertil. Devel., 1993, vol. 5, no. 5, pp. 405–415.
Gianoroli, L., Tosti, E., Magli, C.M., et al., Fertilization Currentin the Human Oocyte, Mol. Reprod. Dev., 1994, vol. 3, pp. 209–214.
Gianoroli, L., Magli, C.M., Ferraretti, A.P., et al., Reducing the Time of Sperm-Oocyte Interaction in Human in vitro Fertilization Improves the Implantation Rate, Hum. Reprod., 1996a, vol. 11, pp. 166–171.
Gianoroli, L., Fiorentino, A., Magli, C.M., et al., Prolonged Sperm-Oocyte Exposure and High Sperm Concentration Affect Human Embryo Viability and Pregnancy Rate, Hum. Reprod., 1996b, vol. 11, pp. 2507–2511.
Krivokharchenko, A.S. and Tatarinova, L.V., Use of Embryos of Variosu Hybrid Murine Lines for Testing Complex Culture Media, Biotekhnologicheskie issledovaniya i perspectivy ikh razvitiya (Biotechnological Studies and Perspectives of Their Development), L'vov, 1990, p. 56.
Krivokharchenko, A.S., Serobyan, G.A., Shakhbazyan, A.K., and Sadovnikov, V.V., Superfast Freezing of Embryos of Murine Outbred and Inbred Lines, Ontogenez, 1996, vol. 27, no. 4, pp. 300–304.
Parrish, J.J., Susko-Parrish, J.L., and First, N.L., Effect of Heparin and Chondroitin Sulfate on the Acrosome Reaction and Fertility of Bovine Sperm in vitro, Theriogenology, 1985, vol. 24, pp. 237–249.
Parrish, J.J., Susko-Parrish, J.L., Leibfried-Rutledge, M.L., et al., Bovine in vitro Fertilization with Frozen-Thawed Semen, Theriogenology, 1986, vol. 25, pp. 591–600.
Plachot, M., Junca, A., Mandelbaum, J., et al., Timing of in vitro Fertilization of Cumulus-Free and Cumulus Enclosed Human Oocytes, Hum. Reprod., 1986, vol. 1, pp. 237–242.
Sumantri, C., Boediono, A., Ooe, M., et al., The Effect of Sperm-Oocyte Time on in vitro Embryo Development Using Sperm from a Tetraparental Chimeric Bull, Anim. Reprod. Sci., 1997, vol. 48, nos. 2–4, pp. 187–195.
Suzuki, O., Asano, T., Yamamoto, Y., et al., Development in vitro of Preimplantation Embryos from 55 Mouse Strains, Reprod. Fertil. Develop., 1996, vol. 8, no. 6, pp. 975–980.
Quinn, P., Lydic, M.L., Ho, M., et al. Confirmation of the Beneficial Effects of Brief Coincubation of Gametes in Human in vitro Fertilization, Fertil. Steril., 1998, vol. 69, no. 3, pp. 399–402.
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Krivokharchenko, A.S., Smetanina, I.G. & Tatarinova, L.V. Fertilization and Subsequent Development of Cattle Oocytes after Reduced Incubation with Spermatozoa. Russian Journal of Developmental Biology 32, 232–235 (2001). https://doi.org/10.1023/A:1016767102172
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DOI: https://doi.org/10.1023/A:1016767102172