Abstract
Relaxation of tensions of the surface of Xenopus laevisembryos at the late blastula stage leads to deep and diverse developmental defects and increased variability in mutual position and volume ratios of the axial rudiments. Here, we demonstrate that the development of such embryos was markedly normalized if the relaxed tensions were restored in one of two ways: (1) isotropic stretching of the blastocoel roof induced by the incubation of relaxed embryos in a hypotonic medium or (2) anisotropic stretching of embryos on two needles. In the latter case, we succeeded in restoring the morphological axis not only after longitudinal stretching but also after transverse stretching, and the new axis had signs of anteroposterior polarity. The role of isotropic and anisotropic tensions in organization of the early amphibian development is discussed.
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REFERENCES
Beloussov, L.V. and Luchinskaya, N.N., Mechanodependent Heterotopies of Axial Rudiments in Xenopus laevis Embryos, Ontogenez, 1995, vol. 26, pp. 213–222.
Beloussov, L.V., Lakirev, A.V., Naumidi, I.I., and Novoselov, V.V., Effects of Relaxation of Mechanical Tensions upon Early Morphogenesis of Xenopus laevis Embryos, Int. J. Dev. Biol., 1990, vol. 34, pp. 409–419.
Beloussov, L.V., Saveliev, S.V., Naumidi, I.I., and Novoselov, V.V., Mechanical Stresses in Embryonic Tissues: Patterns, Morphogenetic Role, and Involvement in Regulatory Feedback, Int. Rev. Cytol., 1994, vol. 150, pp. 1–34.
Ermakov, A.S. and Beloussov, L.V., Variability and Asymmetry of Axial Rudiments in Xenopus laevis Embryos upon Disturbance of Cell Movements and Tension Fields in the Early Gastrula Marginal Zone, Ontogenez, 1998a, vol. 29, pp. 38–46.
Ermakov, A.S. and Beloussov, L.V., Morphogenetic and Differentiation Consequences of Relaxation of Mechanical Tensions in the Xenopus laevis Blastula, Ontogenez, 1998b, vol. 29, pp. 450–458.
Keller, R., Cell Rearrangements in Morphogenesis, Zool. Sci., 1987, vol. 4, pp. 763–779.
Keller, R. and Tibbetts, P., Mediolateral Cell Intercalation in the Dorsal, Axial Mesoderm of Xenopus laevis, Devel. Biol., 1989, vol. 131, pp. 539–549.
Lakirev, A.V., Beloussov, L.V., and Naumidi, I.I., Influence of External Tensions on Tissue Differentiation in Xenopus laevis Embryos in vitro, Ontogenez, 1988, vol. 19, no. 6, pp. 591–600.
Moon, R.T. and Kimelman, D., From Cortical Rotation to Organizer Gene Expression: Towards a Molecular Explanation of Axis Specification in Xenopus, BioEssays, 1998, vol. 20, pp. 536–545.
Newport, J. and Kirschner, M., A Major Developmental Transition in Early Xenopus Embryos, Cell, 1982, vol. 30, pp. 687–703.
Nieuwkoop, P.D. and Faber, J., Normal Table of Xenopus laevis Daudin, Amsterdam: North-Holland Publ. Co., 1956.
Pletjushkina, O.J., Belkin, A.M., Ivanova, O.J., et al., Maturation of Cell-Substratum Foal Adhesions Induced by Depolymerization of Microtubules Is Mediated by Increased Cortical Tension, Cell Adhes. Commun., 1998, vol. 5, pp. 121–135.
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Beloussov, L.V., Ermakov, A.S. Artificially Applied Tensions Normalize Development of Relaxed Xenopus laevisEmbryos. Russian Journal of Developmental Biology 32, 236–241 (2001). https://doi.org/10.1023/A:1016719219011
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DOI: https://doi.org/10.1023/A:1016719219011