Abstract
The cry4Ba gene from Bacillus thuringiensis subsp. israelensis and the binary toxin gene from B. sphaericus C3-41 were cloned together into a shuttle vector and expressed in an acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7. Transformed strain Bt-BW611, expressing both Cry4Ba protein and binary toxin protein, was more than 40-fold more toxic to Culex pipiens larvae resistant to B. sphaericus than the transformed strains expressing Cry4Ba protein or binary toxin protein independently. This result showed that the coexpression of cry4Ba of B. thuringiensis subsp. israelensis with B. sphaericus binary toxin gene partly suppressed more than 10,000-fold resistance of C. pipiens larvae to the binary toxin. It was suggested that production of Cry4Ba protein and binary toxin protein interacted synergistically, thereby increasing their mosquito-larvicidal toxicity.
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Bourgouin, C., Delecluse, A., de la Torre, F. & Szulmajster, J. 1990 Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression. Applied and Environmental Microbiology 56, 340–344.
Brian, H.B. & Jack, T.T. 1989 Transformation of Bacillus cereus vegetative cells by electroporation. Applied and Environmental Microbiology 55, 1649–1652.
Delecluse, A., Poncet, S., Klier, A. & Rapoport, G. 1993 Expression of cryIVA and cryIVB genes independently or in combination in a crystal minus strain of Bacillus thuringiensis subsp. israelensis. Applied and Environmental Microbiology 59, 3922–3927.
Li, T., Sun, F., Yuan, Z., Zhang, Y., Yu, J. & Pang, Y. 2000 Coexpression of cyt1Aa of Bacillus thuringiensis subsp. israelensis with Bacillus sphaericus binary toxin gene in acrystalliferous strain of B. thuringiensis. Current Microbiology 40, 322–326.
Poncet, S., Delecluse, A., Anello, G., Klier, A. & Rapoport, G. 1994 Transfer and expression of the cryIVB and cryIVD genes of Bacillus thuringiensis subsp. israelensis in Bacillus sphaericus 2297. FEMS Microbiology Letters 117, 91–96.
Nielsen-LeRoux, C., Pasquier, F., Charles, J.F., Sinegre, G., Gaven, B. & Pasteur, N. 1997 Resistance to Bacillus sphaericus involves different mechanisms in Culex pipiens larvae. Journal of Medical Entomology 34, 321–337.
Sambrook, J., Fritsch, E.F. & Maniatis, T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn., Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. ISBN 0–87969309–3.
Schurter, W., Geiser, M. & Mathe, D. 1989 Efficient transformation of Bacillus thuringiensis and B. cereus via electroporation: transformation of acrystalliferous strains with a cloned delta-endotoxin gene. Molecular and General Genetics 218, 177–181.
Sinegre, G., et al. 1994 First field occurrence of Culex pipiens resistance to Bacillus sphaericus in southern France. Eighth European Meeting of Society of Vector Ecology. Barecelona, p. 17.
Thiery, I., Hamon, S., Delecluse, A. & Orduz, S. 1998 The introduction into Bacillus sphaericus of the Bacillus thuringiensis subsp. medellin cyt1Ab1 gene results in higher susceptibility of resistant mosquito larvae populations to B. thuringiensis. Applied and Environmental Microbiology 64, 3910–3916.
(WHO) World Health Organization. 1985 Informal consultation on the development of Bacillus sphaericus as microbial lavicide. TDR/BCV/SPHAERICUS/85.3.1–24.
Wirth, M.C. & Georghiou, G.P. 1997 Cross-resistance among CryIV toxins of Bacillus thuringiensis subsp. israelensis in Culex quinquefasciatus (Diptera: Culicidae). Journal of Economical Entomology 90, 1471–1477.
Yuan, Z., Nielsen-LeRoux, C., Pasteur, N. & Frutos, R. 1999 Cloning and expression of the binary toxin genes of Bacillus sphaericus C3–41 in a crystal minus B. thuringiensis subsp. israelensis. Acta Microbiogica Sinica 39, 35–41.
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Sun, F., Yuan, Z., Li, T. et al. Reduction of resistance of Culex pipiens larvae to the binary toxin from Bacillus sphaericus by coexpression of cry4Ba from Bacillus thuringiensis subsp. israelensis with the binary toxin gene. World Journal of Microbiology and Biotechnology 17, 385–389 (2001). https://doi.org/10.1023/A:1016718814180
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DOI: https://doi.org/10.1023/A:1016718814180