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Effects of Gamma-Radiation in Oogenesis of Drosophila Mutants Defective for Repair and Meiotic Recombination

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Abstract

Effects of mutations rad201, mei-9, and mei-41on cell sensitivity to gamma-radiation in Drosophilaoogenesis were studied. Females of the control (Oregon R) and mutant strains were irradiated at a dose of 15 Gy. For 9 days after the irradiation, the number of eggs in consecutive day batches, the frequency of dominant lethals (DLs) among the eggs, and the cytologically recorded distribution of oocytes for stages of their development, and the frequency of egg chamber degeneration in female ovaries were estimated. As a result of joint analysis of the data, different oogenesis stages were characterized with regard to the frequency of two radiation-induced events: appearance of DLs in oocytes and degeneration of egg chambers due to apoptosis of nurse cells. It was shown that the mutations affect these parameters only at particular stages of early oogenesis, at which previtellogenetic growth of egg follicles and meiotic recombination in oocytes occur. Mutation rad201 G1increased the frequency of DLs and egg chamber degeneration, mei-41 D5affected only the DL frequency, and mei-9 a, in addition to enhancing the chamber degeneration frequency, promoted radiation “rescue” of some oocytes from the DL induction.

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REFERENCES

  1. Mason, J.M., Green, M.M., and Shaw, K.E., Genetic Analysis of X-Linked Mutagen-Sensitive Mutants of Drosophila melanogaster, Mutat. Res., 1981, vol. 81, pp. 329-343.

    Google Scholar 

  2. Khromykh, Yu.M., Varentsova, E.R., and Zakharov, I.A., Drosophila Mutants Hypersensitive to Ionizing Radiation, Dokl. Akad. Nauk SSSR, 1977, vol. 234, p. 199.

    Google Scholar 

  3. Baker, B.S. and Carpenter, A.T.C., Genetic Analysis of Sex-Chromosomal Meiotic Mutants in Drosophila melanogaster, Genetics, 1972, vol. 71, pp. 225-286.

    Google Scholar 

  4. Khromykh, Yu.M., Study of the Genetic and Radiobiological Effects of a Radiosensitivity Mutation in Drosophila, Cand. Sci. (Biol.) Dissertation, Leningrad: Leningrad. Gos. Univ., 1982.

    Google Scholar 

  5. Sekelsky, J.J., McKim, K.S., Chin, G.M., and Hawley, R.S., The Drosophila Meiotic Recombination Gene mei-9 Encodes a Homologue of the Yeast Excision Repair Protein Rad1, Genetics, 1995, vol. 141, pp. 619-627.

    Google Scholar 

  6. Hari, K.L., Santerre, A., Sekelsky, J.J., et al., The mei-41 Gene of D. melanogaster Is a Structural and Functional Homolog of the Human Ataxia Telangiectasia Gene, Cell (Cambridge, Mass.), 1995, vol. 82, pp. 815-821.

    Google Scholar 

  7. King, R.C., Rubinson, A.C., and Smith, R.F., Oogenesis in Adult Drosophila melanogaster, Growth, 1956, vol. 20, p. 121.

    Google Scholar 

  8. King, R.C., The Cytology of the Irradiated Ovary of Drosophila melanogaster, Exp. Cell Res., 1957, vol. 13, pp. 545-552.

    Google Scholar 

  9. Khromykh, Yu.M., Study of Radiosensitive Drosophila Lines: 3. The Effect of the rad(2)201G1 Mutation on the Manifestation of Radiation Damage in Female Oocytes, Genetika (Moscow), 1981, vol. 27, no. 4, pp. 667-676.

    Google Scholar 

  10. Varentsova, E.R. and Khromykh, Yu.M., Interaction of Mutations of the mei-9, mei-41, and rad201 Genes for Mutagen Sensitivity in Drosophila Exposed to Ionizing Radiation, Genetika (Moscow), 1997, vol. 33, no. 3, pp. 328-332.

    Google Scholar 

  11. Foley, K. and Cooley, L., Apoptosis in Late-Stage Drosophila Nurse Cells Does Not Require Genes within the H99 Deficiency, Development (Cambridge, UK), 1998, vol. 125, pp. 1075-1082.

    Google Scholar 

  12. Lakin, G.F., Biometriya (Biometry), Moscow: Vysshaya Shkola, 1980.

    Google Scholar 

  13. Abbot, W.S., A Method for Computing the Effectiveness of an Insecticide, J. Econ. Entomol., 1925, vol. 18, pp. 265-267.

    Google Scholar 

  14. Fritz-Niggli, H., Buchi, K., and Schweizer, P., Oxygen Effect as an Inhibition of Repair: Radiation Studies on Excision Repair Deficient mei-9 Embryos of Drosophila, Radiat. Environ. Biophys., 1981, vol. 19, pp. 265-274.

    Google Scholar 

  15. Banga, S.S., Velasquez, A., and Boyd, J.B., P Transposition in Drosophila Provides a New Tool for Analyzing Postreplication Repair and Double-Strand Break Repair, Mutat. Res., 1991, vol. 255, pp. 79-88.

    Google Scholar 

  16. Khromykh, Yu.M. and Kotlovanova, L.V., The rad201 Gene Controls Cell-Cycle Arrest in Irradiated Drosophila, Cells, Genetika (Moscow), 2001, vol. 37, no. 6, pp. 862-864.

    Google Scholar 

  17. Davring, L. and Sunner, M., Early Prophase in Female Meiosis of Drosophila melanogaster, Hereditas (Lund, Swed.), 1976, vol. 82, pp. 129-131.

    Google Scholar 

  18. Sekelsky, J.J., Kenneth, C.B., and Hawley, R.S., Damage Control: The Pleiotropy of DNA Repair Genes in Drosophila melanogaster, Genetics, 1998, vol. 148, pp. 1587-1598.

    Google Scholar 

  19. Roeder, G.S. and Bailis, J.M., The Pachytene Checkpoint, Trends. Genet., vol. 16.

  20. Varentsova, E.R. and Khromykh, Yu.M., The Prolonged Maternal Effect of the Drosophila rad201 Radiosensitivity Gene, Genetika (Moscow), 1997, vol. 33, no. 2, pp. 211-216.

    Google Scholar 

  21. Edgar, B.A. and O'Farrel, P.H., The Three Postblastodermal Cell Cycles of Drosophila Embryogenesis Are Regulated in G2 by string, Cell (Cambridge, Mass.), 1990, vol. 62, pp. 469-480.

    Google Scholar 

  22. Carpenter, A.T.C., Mismatch Repair, Gene Conversion, and Crossing-Over in Two Recombination Defective Mutants of Drosophila melanogaster, Proc. Natl. Acad. Sci. USA, 1982, vol. 79, pp. 5961-5965.

    Google Scholar 

  23. Fishman-Lobell, J. and Haber, E., Removal of Nonhomologous DNA Ends in Double-Strand Break Recombination: The Role of the Yeast Ultraviolet Repair Gene rad1, Science, 1992, vol. 258, pp. 480-484.

    Google Scholar 

  24. Friedberg, E.G., Walker, G.C., and Siede, W., DNA Repair and Mutagenesis, Washington: Am. Soc. Microbiol., 1995.

    Google Scholar 

  25. Henderson, D., DNA Repair Defects and Other Mistakes in Drosophila melanogaster, Methods: A Companion to Methods in Enzymology, 1999, vol. 18, pp. 377-400.

    Google Scholar 

  26. Ilyasov, Yu.I., Savina, V.A., and Shvartsman, P.Ya., Study of the Frequency of Induced Crossing Over at Various Stages of Drosophila Germline Cell Development, Gertsenovskie chteniya (Gertsen Course of Lectures), Leningrad: Izd. LGPI, 1970, p. 147.

    Google Scholar 

  27. Gasior, S., Wong, A., Shinohara, A., and Bishop, D., Rad52 Associates with RPA and Functions with Rad55 and Rad57 to Assemble Meiotic Recombination Complex, Genes Dev., 1998, vol. 12, pp. 2208-2221.

    Google Scholar 

  28. Sarantseva, S.V., Study of the Effects of a Mutagen Sensitivity Mutation in Embryogenesis and Oogenesis of Drosophila melanogaster, Cand. Sci. (Biol.) Dissertation, St. Petersburg: St. Petersburg Gos. Univ., 1999.

    Google Scholar 

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  1. Konstantinov Institute of Nuclear Physics, Gatchina, Leningrad oblast, 188350 Russia

    S. V. Sarantseva & Yu. M. Khromykh

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  1. S. V. Sarantseva
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  2. Yu. M. Khromykh
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Sarantseva, S.V., Khromykh, Y.M. Effects of Gamma-Radiation in Oogenesis of Drosophila Mutants Defective for Repair and Meiotic Recombination. Russian Journal of Genetics 37, 631–638 (2001). https://doi.org/10.1023/A:1016669123397

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