Abstract
Reciprocal chromosome painting and G-banding were used to compare the karyotypes of three Australian marsupials (Sminthopsis crassicaudata, Macropus eugenii, Trichosurus vulpecula) and one South American marsupial (Monodelphis domestica). The results revealed only a limited number of rearrangements between these species and that the four karyotypes can be described as different combinations of fifteen conserved segments. Five chromosomes are totally conserved between M. domestica (pairs 1, 2, 5, 8 and the X) and the presumed 2n = 14 Australian ancestral karyotype, while M. domestica pairs 3 and 6 and 4 and 7 would have been involved in fusion/fission rearrangements. Chromosome comparisons are presented in a chromosome homology map. Although the species studied diverged 70 million years ago, the karyotype of Monodelphis domestica is highly conserved in relation to those of Australian marsupials.
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References
Arnold N, Stanyon R, Jauch A, O'Brien PCM, Wienberg J (1996) Identification of complex chromosome rearrangements in the gibbon by fluorescence in-situ hybridization (FISH) of a human chromosome 2q specific microlibrary, yeast artificial chromosomes, and reciprocal chromosome painting. Cytogenet Cell Genet 74: 80–85.
De Leo AA, Guedelha N, Toder R et al. (1999) Comparative chromosome painting between marsupial orders: Relationships with a 2n. 14 ancestral marsupial karyotype. Chromosome Res 7: (7) 509–517.
Eldridge MDB, Close RL (1993) Radiation of chromosome shuffles. Curr Opin Genet Dev 3: 915–922.
Eldridge MDB, Johnston PG, Close RL (1992) Chromosomal rearrangements in rock wallabies, Petrogale (Marsupiala, Macropodidae). Cytogenet Cellgenet 61: 915–922.
Ferguson-Smith MA (1997) Genetic analysis by chromosome sorting and painting: phylogenetic and diagnostic applications. Eur J Hum Genet 5: 253–265.
Ferguson-Smith MA, Yang F, O'Brien PCM (1998) Comparative mapping using chromosome sorting and painting. ILAR J 39: 68–76.
Glas R, De Leo AA, Delbridge ML et al. (1999) Chromosome painting in marsupials: genome conservation in the kangaroo family. Chromosome Res 7: 167–176.
Goureau A, Yerle, M. Schmitz A et al. (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36: 252–262.
Hayman DL, Martin PG (1974) Marsupial Cytogenetics. Berlin: Gebruder Borntraeger.
Kirsch JAW, Lapointe FL, Springer MS (1997) DNA-hybridisation studies of marsupials and their implication for metatherian classification. Aust J Zool 45: 211–280.
Koehler U, Bigoni F, Wienberg J, Stanyon R (1995) Genomic reorganization in the concolor gibbon (Hylobates concolor) revealed by chromosome painting. Genomics 30: 287–292.
McKay LM, Watson JM, Graves JAM (1992) Mapping human X-linked genes in the Phalangerid marsupial Trichosurus vulpecula. Genomics 14: 302–308.
O'Brien SJ, Wienberg J, Lyons LA (1997) Comparative genomics: lessons from cats. Trends Genet Vol 13 10: 393–399
O'Brien SJ, Menotti-Raymond M, Murphy WJ et al. (1999) The promise of comparative genomics in mammals. Science 286: 458–481.
O'Neill RJW, Eldridge MDB, Ferguson-Smith MA, O'Brien PC, Graves JAM (1999) Chromosome evolution in kangaroos (Marsupialia:Macropodidae). Cross species chromosome painting between the tammar wallaby and rock wallaby spp. with the 2n. 22 ancestral macropodid karyotype. Genome 42: 525–530.
Pathak S, RÖnne M, Brown NM, Furlong CL, VandeBerg JL (1993) A high resolution banding pattern idiogram of Monodelphis domestica chromosomes (Marsupiala, Mammalia). Cytogenet Cell Genet 63: 181–184.
Rens W, O'Brien PCM, Yang F, Graves JAM, Ferguson-Smith (1999) Karyotype relationships between four distantly related marsupials revealed by reciprocal chromosome painting. Chromosome Res 7: 461–474
Rofe RH (1978) G-banded chromosomes and the evolution of Macropodidae. Aust. Mammal 2: 53–63.
Rofe R, Hayman D (1985) G-banding evidence for a conserved complement in the Marsupiala. Cytogenet Cell Genet 39: 40–50.
Svartman M, Vianna-Morgante AM (1998) Karyotype evolution of marsupials: from higher to lower diploid numbers. Cytogenet Cell Genet 82: 263–266.
Svartman M, Vianna-Morgante AM (1999) Comparative genome analysis in American marsupials: chromosome banding and in-situ hybridization. Chromosome Res 7: 267–275.
Toder R, Wienberg J, Voullaire L, O'Brien PCM, Maccarone P, Graves JAM (1997a) Shared DNA sequences between the X Karyotype relationships between marsupials 307 and Y chromosomes in the tammar wallaby-evidence for independent additions to eutherian and marsupial sex chromosomes. Chromosoma 106: 94–98.
Toder R, O'Neill RJW, Wienberg J, O'Brien PCM, Voullaire L, Graves JAM (1997b) Comparative chromosome painting between two marsupials; origin of an XX/XY1Y2 sex chromosome system. Mammalian Genome 8: 347–350.
Toder R, O'Neill RJW, Graves JAM (1998) Chromosome painting in marsupials. ILAR J 39: 92–95.
Wienberg J, Stanyon R (1997) Comparative painting of mammalian chromosomes. Curr Opin Genet Dev 7: 784–791.
Yang F, O'Brien PCM, Milne BS et al. (1999) A complete comparative map for the dog, red fox and human and its integration with canine genetic maps. Genome 62: 189–202.
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Rens, W., O'Brien, P.C.M., Yang, F. et al. Karyotype relationships between distantly related marsupials from South America and Australia. Chromosome Res 9, 301–308 (2001). https://doi.org/10.1023/A:1016646629889
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DOI: https://doi.org/10.1023/A:1016646629889