Abstract
One hundred and eighteen lactic acid bacteria (LAB) were isolated from five different types of ragi tape, a traditional dry-starter of Balinese rice wine. The isolates could be classified into three groups based on the cell shape and capability to produce gas from glucose. Group I contained 66 homofermentative cocci, group II contained seven homofermentative rods, and group III contained 45 heterofermentative rods. Among these 118 isolates, 21 isolates representing these groups were selected and were first identified using phenotypic characters. The identification performed phenotypically was confirmed by sequencing of variable region 8 (V8) of the 16S rDNA. The comparative studies led to the identification of Pediococcus pentosaceus, Enterococcus faecium, Lactobacillus curvatus, Weissella confusa, and W. paramesenteroides from the ragi tape examined.
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Ardana, M.M. & Fleet, G.H. 1989 The microbial ecology of tape ketan fermentation. International Journal of Food Microbiology 9, 157–165.
Bousfield, I.J., Keddie, R.M., Dando, T.R. & Shaw, S. 1985 Simple rapid methods of cell wall analysis as an aid in the identification of aerobic coryneform bacteria. In Chemical Methods in Bacterial Systematic, ed. Goodfellow, M. & Minnikin, D.E. pp. 222–234. London and New York: Academic Press. ISBN 0–12–289675–0.
Collins, M.D., Samelis, J., Metaxopoulos, J. & Wallbanks, S. 1993 Taxonomic studies on some Leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. Journal of Applied Bacteriology 75, 595–603.
Cronk, T.C., Steinkraus, K.H., Hackler, L.R. & Mattick, L.R. 1977 Indonesian tape ketan fermentation. Applied and Environmental Microbiology 33, 1067–1073.
Dellaglio, F., Dicks, L.M.T. & Torriani, S. 1995 The genus Leuconostoc. In The Genera of Lactic Acid Bacteria, ed. Wood, B.J.B. & Holzapfel, W.H. pp. 235–278. London: Blackie Academic & Professional. ISBN 0–7514–0215-X.
Devriese, L.A. & Pot, B. 1995 The genus Enterococcus. In The Genera of Lactic Acid Bacteria, ed. Wood, B.J.B. & Holzapfel, W.H. pp. 327–367. London: Blackie Academic & Professional. ISBN 0–7514–0215-X.
Dick, L.M.T., Dellaglio, F. & Collins, M.D. 1995 Proposal to reclassify Leuconostoc oenos as Oenococcus oeni [corrig.] gen. nov., comb. nov. International Journal of Systematic Bacteriology 45, 395–397.
Djien, K.S. 1972 Tape fermentation. Applied Microbiology 23, 976–978.
Dwijosepoetro, D. & Wolf, F.T. 1970 Microbiological studies of Indonesian fermented foodstuffs. Mycopathologia et Mycologia Applicata 41, 211–222.
Gancel, F., Dzierszinski, F. & Taillez, R. 1997 Identification and characterization of Lactobacillus species isolated from fillets of vacuum-packed smoked and salted herring (Clupea harengus). Journal of Applied Microbiology 82, 722–728.
Garvie, E.I. 1986a Request for an opinion. Conservation of the name Pediococcus acidilactici with DSM 20284 as the neotype strain and rejection of previous neotype strain NCDO 1859 (=IFO 3884=DSM 20333=ATCC 33314). International Journal of Systematic Bacteriology 36, 579–580.
Garvie, E.I. 1986b Genus Leuconostoc. In Bergey's Manual of Systematic Bacteriology, Vol. 2, ed. Sneath, P.H.A., Mair, N.S., Sharpe, M.E. & Holt, J.G. pp. 1071–1075. Baltimore: Williams and Wilkins. ISBN 0–683–07893–3.
Hadisepoetro, E.S.S., Takada, N. & Oshima, Y. 1979 Microflora in ragi and usar. Journal of Fermention Technology 57, 251–259.
Hammes, W.P. & Vogel, R.F. 1995 The genus Lactobacillus. In The Genera of Lactic Acid Bacteria, ed. Wood, B.J.B. & Holzapfel, W.H. pp. 19–55. London: Blackie Academic & Professional. ISBN 0–7514–0215-X.
Hesseltine, C.W. 1965 A millennium of fungi, food, and fermentation. Mycologia 2, 149–198.
Hesseltine, C.W. 1983 Microbiology of oriental fermented foods. Annual Reviews of Microbiology 37, 575–601.
Hesseltine, C.W. & Ray, M.L. 1988 Lactic acid bacteria in muncha and ragi. Journal of Applied Bacteriology 64, 395–401.
Ishimaru, Y. & Nakano, M. 1960 Studies on the microorganisms in the ragi. Report of Food Research Institute 18, 158–163 (in Japanese).
Kodama, K. 1970 Sake Yeasts. In The Yeasts, Vol. 3. Yeast Technology, ed. Rose, A.H. & Harrison, J.S. pp. 225–274. London and New York: Academic Press. ISBN 0–12–596403-X.
Kozaki, M., Uchimura, Y. & Okada, S. 1992 Manual for Isolation and Identification of Lactic Acid Bacteria. Tokyo: Asakura Shoten. ISBN 4–254–43049–3 (in Japanese).
Kuriyama, H., Sastraatmadja, D., Igosaki, Y., Watanabe, K., Kanti, A. & Fukatsu, T. 1997 Identification and characterization of yeast isolated from Indonesian fermented food. Mycoscience 38, 441–445.
Manome, A., Okada, S., Uchimura, T. & Komagata, K. 1998 The ratio of L-form to D-form of lactic acid as a criteria for the identification of lactic acid bacteria. Journal of General and Applied Microbiology 44, 371–374.
Montel, M.C., Talon, R., Fornaud, J. & Champomier, V. 1991 A simplified key for identification of homofermentative Lactobacillus and Carnobacterium spp. from Meat. Journal of Applied Bacteriology 70, 469–472.
Mori, K. 1996 Identification of lactic acid bacteria by sequencing of 16S rDNA. Bulletin of Japanese Study Group for Lactic Acid Bacteria 6, 80–84 (in Japanese).
Neef, J.M., Van de Peer, Y., Hendriks, L. & Watcher, D.R. 1990 Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Research 18(supplement), 2237–2317.
Otsuka, M., Okada, S., Uchimura, T. & Komagata, K. 1994 A simple method for the determination of stereoisomers of lactic acid by HPLC using anenantiomeric resolution column, and its application to identification of lactic acid bacteria. Seibutsu-kogaku Kaishi 72, 81–86 (in Japanese).
Rohif, F.J. 1995 NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Ver.1.80. Exeter Software. New York.
Sakurai, H., Yokota, A. & Tomita, F. 1997 Molecular cloning of an inulin fructotransferase (depolymerizing) gene from Arthrobacter sp. H-657 and its expression in Escherichia coli. Bioscience Biotechnology and Biochemistry 61, 87–92.
Schleifer, K.H. & Kilpper-Bälz, R. 1984 Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. International Journal of Systematic Bacteriology 34, 31–34.
Simpson, W.J. & Taguchi, H. 1995 The genus Pediococcus with notes on the genera Tetragenococcus and Aerococcus. In The Genera of Lactic Acid Bacteria, ed. Wood, B.J.B. & Holzapfel, W.H. pp. 125–172. London: Blackie Academic & Professional. ISBN 0–7514–0215-X.
Sone, T., Abe, T., Yoshida, N., Suto, M. & Tomita, F. 1997 DNA fingerprinting and electrophoretic karyotyping of Japanese isolates of rice blast fungus. Annals of the Phytopathological Society of Japan 63, 155–163.
Sperber, W.H. & Swan, J. 1976 Hot-loop test for the determination of carbon dioxide production from glucose by lactic acid bacteria. Applied and Environmental Microbiology 31, 990–991.
Steinkraus, K.H. 1996 Indigenous fermented foods in which ethanol is a major product. In Handbook of Indigenous Fermented Foods, 2nd edn. ed. Steinkraus, K.H. pp. 363–508. New York: Marcel Dekker, Inc. ISBN 0–8247–9352–8.
Ulrich, U. & Müller, T. 1998 Heterogeneity of plant-associated Streptococci as characterized by phenotypic features and restriction analysis of PCR-amplified 16S rDNA. Journal of Applied Microbiology 84, 298–303.
Wibowo, D., Eschenbruch, R., Davis, C.R., Fleet, G.H. & Lee, T.H. 1985 Occurrence and growth of lactic acid bacteria in wine. American Journal of Enology and Viticulture 36, 302–313.
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Sujaya, I., Amachi, S., Yokota, A. et al. Identification and characterization of lactic acid bacteria in ragi tape. World Journal of Microbiology and Biotechnology 17, 349–357 (2001). https://doi.org/10.1023/A:1016642315022
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DOI: https://doi.org/10.1023/A:1016642315022