Abstract
Statistical test designs were used to identify and quantify the soil parameters important for determining the ecotoxicological effects of copper and lead to the potworm Enchytraeus albidus. The application of a Fractional Factorial Design revealed that the acute toxicity of copper and lead to E. albidus can vary over more than two orders of magnitude depending on the physico-chemical characteristics of the (artificial) soils. The differences in metal ecotoxicity were mainly determined by pH and organic matter content or cation exchange capacity (CEC). Using a Central Composite Design, models were developed describing the ecotoxicity of copper and lead to E. albidus as a function of these parameters. To validate the developed response surface models, two field soils and the standard artificial soil prescribed by OECD were spiked with copper and lead and the acute toxicity to E. albidus was assessed. These validation experiments confirmed that the toxicity of copper and lead could be predicted using information on the total metal concentration, the pH and the CEC of the soil with toxicity decreasing with increasing pH and CEC.
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Ameryckx, J.B., Verheye, W. and Vermeire, R. (eds.) (1995). Bodemkunde, 346 pp. Gent, Belgium.
Belfroid, A., Sikkenk, M., Seinen, W., Van Gestel, K. and Hermens, J. (1994). The toxicokinetic behavior of chloro-benzenes in earthworm Eisenia andrei experiments in soil. Environ. Toxicol. Chem. 13, 93–9.
Buchter, B., Davidoff, B., Amacher, M.C., Hinz, C., Iskandar, I.K. and Selim, H.M. (1989). Correlation of Freundlich Kd and n retention parameters with soils and elements. Soil Sci. 148, 370–9.
Chhabra, R., Pleysier, J. and Cremers, A. (1975). The measurement of the cation exchange capacity and exchangeable cations in soils: A new method. In S.W. Bailly (ed) Proceedings of the International Clay Conference in Mexico, pp. 439–49. Mexico City: Applied Publications Limited.
Harter, R.D. (1983). Effects of soil pH on adsorption of lead, copper, zinc and nickel. Soil Sci. Soc. Am. J. 47, 47–51.
ISO, International Organisation for Standardisation (1994). Soil quality Determination of pH, No. 10390. Geneve: ISO.
ISO, International Organisation for Standardisation (1996). Soil quality Effects of pollutants on earthworms Eisenia fetida. Part 2: Determination of effects on reproduction, No. 11268–2. Geneve: ISO.
Jäggy, A. and Streit, B. (1982). Toxic effects of suluble copper on Octalasium cyaneum Sav. Lumbricidae. Revue Suisse Zool. 89, 881–9.
Janssen, R.P.T., Peijnenburg, W.J.G.M., Posthuma, L. and Van Den Hoop, M.A.G.T. (1997a). Equilibrium partitioning of metals in Dutch field soils. I. Relationship between metal partition coefficients and soil characteristics. Environ. Toxicol. Chem. 16, 2470–8.
Janssen, R.P.T., Posthuma, L., Baerselman, R., Den Hollander, H.A., Van Veen, R.P.M. and Peijnenburg, W.J.G.M. (1997b). Equilibrium partitioning of heavy metals in Dutch field soils. II. Prediction of metal accumulation in earthworms. Environ. Toxicol. Chem. 16, 2479–88.
Korthals, G.W., Alexey, A.D., Lexmond, T.M., Kammenga, J.E. and Bongers, T. (1996). Long-term effects of copper and pH on the nematode community in an agroecosystem. Environ. Toxicol. Chem. 15, 979–85.
OECD, Organisation for Economic Cooperation and Development (1984). Earthworm, acute toxicity tests, Guideline No. 207. Paris: OECD.
OECD, Organisation for Economic Cooperation and Development (1999). Enchytraeid reproduction test. Draft Guideline No. 220. Paris: OECD.
Spurgeon, D.J. and Hopkin, S.P. (1996). Effects of variations of the organic matter content and pH of soils on the availability and toxicity of zinc to the earthworm Eisenia fetida. Pedobiologia 40, 80–96.
Statistica (1994). Experimental design. Statistica Volume III, pp. 3613–82. Tulsa: Statsoft.
Stephan, C. (1977). Methods for Calculating an LC50, Special Technical Publication, vol. 34, pp. 65–84. ASTM American Society for Testing and Materials.
Van den Hoop, M.A.G.T. (1995). Metal speciation in Dutch soils: Field-based partition coefficients for heavy metals at background levels. Report 719101013. Bilthoven, The Netherlands: National Institute for Public Health and the Environment.
Van Gestel, C.A.M., Rademaker, M.C.J. and van Straalen, N.M. (1995). Capacity controlling parameters and their impact on metal toxicity. In W. Salomons and W.M. Stigliani (eds) Biogeodynamics of Pollutants in Soils and Sediments, pp. 171–92. Springer-Verlag.
Van Straalen, N.M. and Bergema, W.F. (1995). Ecological risks of increased bioavailability of metals under soil acidification. Pedobiologia 39, 1–9.
Walkley, A. and Black, I.A. (1934). An examination of the Degtjareff for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 37, 29–38.
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Lock, K., Janssen, C.R. Test Designs to Assess the Influence of Soil Characteristics on the Toxicity of Copper and Lead to the Oligochaete Enchytraeus albidus. Ecotoxicology 10, 137–144 (2001). https://doi.org/10.1023/A:1016633725412
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DOI: https://doi.org/10.1023/A:1016633725412