Abstract
The 27-bp tandem repeat polymorphism in intron 4 of the endothelial nitric oxide synthase gene (eNOS) in populations of the Volga–Ural region was studied by means of polymerase chain reaction. In Russians and Tatars, the possible association of this polymorphism with coronary heart disease complicated by either myocardial infarction or by essential hypertension was examined. Russians with essential hypertension associated with hypertrophy of the left ventricle displayed a statistically significant increase of the eNOS4A/Bgenotype and theAallele frequencies along with the decrease of the frequencies of the eNOS4B/Bgenotype and the Ballele. In Tatars survived from myocardial infarction and with the risk of cardiovascular diseases (smoking or burdened heredity), a statistically significant increase of the frequencies of the eNOS4A/Bgenotype and the Aallele was observed. Thus, in Russians the eNOS4A/Bgenotype was associated with the development of essential hypertension, while in Tatars it was associated with the risk of myocardial infarction.
Similar content being viewed by others
REFERENCES
Wang, X.L., Sim, A.S., Badenhop, R.F., et al., A Smoking-Dependent Risk of Coronary Artery Disease Associated with the Polymorphism of Endothelial Nitric Oxide Synthase Gene, Nat. Med., 1996, vol. 2, pp. 41-45.
Hooper, W.C., Lally, C., Austin, H., et al., The Relationship between Polymorphisms in the Endothelial Nitric Oxide Synthase Gene and the Platelet GPIIIa Gene with Myocardial Infarction and Venous Thromboembolism in African Americans, Chest, 1999, vol. 116, no. 4, pp. 880-886.
Smith, P.D., Steeds, R., Channer, K., and Samani, N.J., Constitutive Endothelial Nitric Oxide Synthase Gene Polymorphism and Risk of Myocardial Infarction, XX Congr. of Eur. Soc. Cardiol., 1998, abstract 2063.
Ishihara, S., Yamada, Y., Fujimura, T., and Yokota, M., Association of Polymorphism of the Endothelial Constitutive Nitric Oxide Synthase Gene with Myocardial Infarction in Japanese Population, Am. J. Cardiol., 1998, vol. 81, pp. 83-86.
Nakagami, H., Ikeda, U., Maeda, J., et al., Coronary Artery Disease and Endothelial Nitric Oxide Synthase and Angiotensin-Converting Enzyme Gene Polymorphisms, J. Thromb. Thrombolysis, 1999, vol. 8, pp. 191-195.
Miyamoto, Y., Saito, Y., Kajiyama, N., et al., Endothelial Nitric Oxide Synthase Gene Is Positively Associated with Essential Hypertension, Hypertension, 1998, vol. 32, pp. 3-8.
Nakayama, M., Soma, M., Takahashi, Y., et al., Association Analysis of CA Repeat Polymorphism of Endothelial Nitric Oxide Synthase Gene with Essential Hypertension, Clin. Genet., 1997, vol. 51, pp. 26-30.
Wang, Y., Kikuchi, S., Suzuki, H., et al., Endothelial Nitric Oxide Synthase Gene Polymorphism in Intron 4 Affects Progression of Renal Failure in Nondiabetic Renal Diseases, Nephrol. Dial. Transplant., 1999, vol. 14, pp. 2898-2902.
Morita, T., Ito, H., Suehiro, T., et al., Effect of Polymorphism of Endothelial Nitric Oxide Synthase Gene in Japanese Patients with IgA Nephropathy, Clin. Nephrol., 1999, vol. 52, no. 4, pp. 203-209.
Yahashi, Y., Kario, K., Shimada, K., and Matsuo, M., The 27-bp Repeat Polymorphism in Intron 4 of the Endothelial Nitric Oxide Synthase Gene and Ischemic Stroke in a Japanese Population, Blood Coagul. Fibrinol., 1998, vol. 9, pp. 405-409.
Park, J.E., Hwang, T.H., Chu, J.A., et al., The Association between a Polymorphism of the Endothelial Nitric Oxide Synthase Gene and the Risk of Acute Myocardial Infarction in the Young Population, 47th An. Scientific Session of Am. Coll. Cardiol., 1998, abstract 1233.
Akar, N., Akar, E., Cin, S., et al., Endothelial Nitric Oxide Synthase Intron 4, 27-bp Repeat Polymorphism in Turkish Patients with Deep Vein Thrombosis and Cerebrovascular Accidents, Thrombosis Res., 1999, vol. 94, pp. 63-64.
Puzyrev, K.V., Clinical Genetic Study of Factors Predisposing to Essential Hypertension and Idiopathic Hypertrophic Cardiomyopathy, Cand. Sci. (Med.) Dissertation, Tomsk: Tomsk Research Center, Russian Academy of Medical Science, 1999.
Minushkina, L.O., Zateishchikov, D.A., and Sidorenko, B.A., Genetic Aspects of the Regulation of Endothelial Function in Arterial Hypertension, Kardiologiya, 2000, no. 3, pp. 68-76.
Soma, M., Nakayama, T., and Kanmatsuse, K., Nitric Oxide Synthase Gene Polymorphism and Its Influence on Cardiovascular Disease, Curr. Opin. Nephrol. Hypertens., 1999, vol. 8, pp. 83-87.
Wang, X.L., Mahaney, M.C., Sim, A.S., et al., Genetic Contribution of Endothelial Constitutive Nitric Oxide Synthase Gene to Plasma Nitric Oxide Levels, Atheroscl. Thromb. Vasc. Biol., 1997, vol. 17, pp. 3147-3153.
Tsukada, T., Yokoyama, K., Arai, T., et al., Evidence of Association of the ecNOS Gene Polymorphism with Plasma NO Metabolite Level in Humans, Biochem. Biophys. Res. Commun., 1998, vol. 245, pp. 190-193.
Johns, M.B. and Paulus-Thomas, J.E., Purification of Human Genomic DNA from Whole Blood Using Proteinase K Treatment Followed by Phenol-Chloroform Extraction, Anal. Biochem., 1989, vol. 180, pp. 276-278.
Roff, P.A. and Bentzen, P., The Statistical Analysis of mtDNA Polymorphism: χ2 and Problem of Small Samples, Mol. Biol. Evol., 1989, vol. 6, pp. 539-545.
Zhivotovsky, L.A., Populyatsionnaya biometriya (Population Biometry), Moscow: Nauka, 1984.
Schlesselman, J., Case-Control Studies: Design, Conduct, Analysis, New York: Oxford Univ. Press, 1982, pp. 58-96.
Hapody Povolzh'ya i Priural'ya (Ethnoses of the Volga and Ural Regions), Kuzeev, R.G., Ed., Moscow: Nauka, 1985.
Khusnutdinova, E.K., Molekulyarnaya etnogenetika narodov Volgo-Ural'skogo regiona (Molecular Ethnogenetics of Ethnoses of the Volga-Ural Region), Ufa: Gilem, 1999.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mustafina, O.E., Shagisultanova, E., Nasybullin, T. et al. Endothelial Nitric Oxide Synthase Gene Minisatellite Polymorphism in Populations of the Volga–Ural Region and Analysis of Its Association with Myocardial Infarction and Essential Hypertension. Russian Journal of Genetics 37, 546–552 (2001). https://doi.org/10.1023/A:1016627017461
Issue Date:
DOI: https://doi.org/10.1023/A:1016627017461