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Enhancing Detection Sensitivity in Capillary Electrophoresis

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Abstract

A simple technique was proposed for improving detection sensitivity in capillary electrophoresis by the chemical etching of the interior surface of a fused-silica capillary in the detection region. This technique was applied to the electrophoresis of proteins. The sensitivity was found to increase, on the average, by a factor of 4 to 5 after etching a 75-μm i.d. capillary with an HF solution for 7 min.

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Authors and Affiliations

  1. Andronikashvili Institute of Physics, Academy of Sciences of Georgia, ul. Tamarashvili 6, Tbilisi, 380077, Georgia

    L. M. Mosulishvili, V. A. Barnov & N. Ya. Tsibakhashvili

  2. University of Saarland, P.O. Box 151150, 66041, Saarbruecken, Germany

    H. Engelhardt & W. Beck

Authors
  1. L. M. Mosulishvili
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  2. V. A. Barnov
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  3. N. Ya. Tsibakhashvili
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  4. H. Engelhardt
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  5. W. Beck
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Mosulishvili, L.M., Barnov, V.A., Tsibakhashvili, N.Y. et al. Enhancing Detection Sensitivity in Capillary Electrophoresis. Journal of Analytical Chemistry 56, 512–514 (2001). https://doi.org/10.1023/A:1016616307737

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  • DOI: https://doi.org/10.1023/A:1016616307737

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