Abstract
Segments of distal and proximal parts of the cotyledons, hypocotyls and epicotyls of yellow pitaya [Selenicereus megalanthus (Cactaceae)], were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). The proximal part of the cotyledons was found to be the most responsive tissue, maximal bud regeneration being achieved in the presence of 200 μM thidiazuron. However, TDZ does not change the pattern of regeneration competence, which remains restricted to the proximal part of the cotyledons. The best medium for shoot elongation was found to be MS lacking plant growth regulators. Rooting was induced on MS medium supplemented with 5.3 μM naphthaleneacetic acid. Rooted plantlets were transferred to a soil medium and acclimatized successfully in a greenhouse.
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Pelah, D., Kaushik, R.A., Mizrahi, Y. et al. Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron. Plant Cell, Tissue and Organ Culture 71, 81–84 (2002). https://doi.org/10.1023/A:1016585108094
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DOI: https://doi.org/10.1023/A:1016585108094