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Heat stress pretreatment mitigates postischemic arachidonic acid accumulation in rat heart

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Abstract

Heat stress pretreatment of the heart is known to protect this organ against an ischemic/reperfusion insult 24 h later. Degradation of membrane phospholipids resulting in tissue accumulation of polyunsaturated fatty acids, such as arachidonic acid, is thought to play an important role in the multifactorial process of ischemia/reperfusion-induced damage.

The present study was conducted to test the hypothesis that heat stress mitigates the postischemic accumulation of arachidonic acid in myocardial tissue, as a sign of enhanced membrane phospholipid degradation. The experiments were performed on hearts isolated from rats either 24 h after total body heat treatment (42°C for 15 min) or 24 h after sham treatment (control). Hearts were made ischemic for 45 min and reperfused for another 45 min.

Heat pretreatment resulted in a significant improvement of postischemic hemodynamic performance of the isolated rat hearts. The release of creatine kinase was reduced from 30 ± 14 (control group) to 17 ± 5 units/g wet wt per 45 min (heat-pretreated group) (p < 0.05). Moreover, the tissue content of the inducible heat stress protein HSP70 was found to be increased 3-fold 24 h after heat treatment. Preischemic tissue levels of arachidonic acid did not differ between heat-pretreated and control hearts. The postischemic ventricular content of arachidonic acid was found to be significantly reduced in heat-pretreated hearts compared to sham-treated controls (6.6 ± 3.3. vs. 17.8 ± 12.0 nmol/g wet wt). The findings suggest that mitigation of membrane phospholipid degradation is a potential mechanism of heat stress-mediated protection against the deleterious effects of ischemia and reperfusion on cardiac cells.

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Authors and Affiliations

  1. Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands

    Ger J. van der Vusse, Richard N. Cornelussen, Theo H.M. Roemen & Luc H.E.H. Snoeckx

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  1. Ger J. van der Vusse
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  2. Richard N. Cornelussen
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  3. Theo H.M. Roemen
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  4. Luc H.E.H. Snoeckx
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van der Vusse, G.J., Cornelussen, R.N., Roemen, T.H. et al. Heat stress pretreatment mitigates postischemic arachidonic acid accumulation in rat heart. Mol Cell Biochem 185, 205–211 (1998). https://doi.org/10.1023/A:1016574720342

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