Skip to main content
SpringerLink
Log in

Induction of somatic embryogenesis and adventitious shoots from immature leaves of cassava

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Direct somatic embryogenesis was successfully achieved from immature leaves of cassava (Manihot esculenta Crantz) cultured on induction medium containing 2,4-dichlorophenoxyacetic acid or naphthaleneacetic acid. Changing the duration of induction or changing plant growth regulators resulted in differences in regeneration of somatic embryos or adventitious shoots. The results showed that auxin was a key factor for inducing embryogenic cells. The embryogenic cells were mainly induced within 4–12 days. Only if the embryogenic cells were induced, the auxin enhanced formation of somatic embryo whereas 6-benzylaminopurine stimulated development of adventitious shoots. Histological examinations supported the conclusion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Christianson ML & Warnick DA (1983) Competence and determination in the process of in vitro shoot organogenesis. Dev. Biol. 95: 288–293

    Google Scholar 

  • Gonzalez C, Schopke C, Taylor NJ, Beachy RN & Fauquet C (1998) Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures. Plant Cell Rep. 17: 827–831

    Google Scholar 

  • Guo JY & Liu YQ (1995) Rapid propagation of cassava by tissue culture and its application in rural districts in China. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN), Bogor, Indonesia, 22-26 August 1994. Vol. I (pp 183–189) CIAT, Cali, Colombia

    Google Scholar 

  • Kuijpers AM, Bouman H & De Klerk GJ (1996) Increase of embryogenic callus formation in cucumber by initial culture on high concentration of 2,4-dichlorophenoxyacetic acid. Plant Cell Tiss. Org. Cult. 46: 81–83

    Google Scholar 

  • Li HQ, Guo JY, Huang YW, Liang CY, Liu HX, Potrykus I & Puousti-Kaerlas S (1998) Regeneration of cassava plant via shoot organogenesis. Plant Cell Rep. 17: 410–414

    Google Scholar 

  • Li HQ, Sautter C, Potrykus I & Puonti-Kaerlas J (1996) Genetic transformation of cassava (Manihot esculenta Crantz). Nature Biotechn. 14: 736–740

    Google Scholar 

  • Ma GH, Xu QS & Xian YL (1998) Direct primary somatic embryogenesis and shoot formation from immature leaves of Manihot esculenta. Acta Bot. Sin. 40: 503–507

    Google Scholar 

  • Ma GH, Xu QS & Xian YL (1999) Effects of several auxins on somatic embryogenesis and plant regeneration in cassava. J. Trop. Subtrop. Bot 7: 75–80

    Google Scholar 

  • Ma GH (1998) Effects of cytokinins and auxins on cassava shoot organogenesis and somatic embryogenesis from somatic embryo explants. Plant Cell Tiss. Org. Cult. 54: 1–7

    Google Scholar 

  • Mathew H, Schopke C, Carcamo R, Chavarriaga P, Fauquet C & Beachy RN (1993) Improvement of somatic embryogenesis and plant recovery in cassava. Plant Cell Rep. 12: 328–333

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Raemakers CJJM, Schavemaker CM, Jacobsen E & Visser RGF (1993) Improvements of cyclic somatic embryogenesis of cassava (Manihot esculenta Crantz). Plant Cell Rep. 12: 226–229

    Google Scholar 

  • Raemakers CJJM, Jacobsen E & Visser RGF (1994) Histology of somatic embryogenesis and evaluation of somaclonal variation. In: Proceedings of the Second International Scientific Meeting of the Cassava Biotechnology Network, Bogor, Indonesia, 22-26 August 1994. Vol I (pp 336–354) CIAT, Cali, Colombia. 1995

    Google Scholar 

  • Sarria R, Torres E, Angel F, Chavarriaga P & Roca WM (2000) Transgenic plants of cassava (Manihot esculenta Crantz) with resistance to Basta obtained by Agrobacterium-mediated transformation. Plant Cell Rep. 19: 339–344

    Google Scholar 

  • Sofiari E, Raemakers CJJM, Kanju E, Danso K, Lammeren AMV, Jacobsen E & Visser RGF (1997) Comparison of NAA and 2,4-D induced somatic embryogenesis in Cassava. Plant Cell Tiss. Org. Cult. 50: 45–56

    Google Scholar 

  • Stamp JA & Henshaw GG (1982) Somatic embryogenesis in cassava. Z. Pflanzenphysiol. 105: 183–187

    Google Scholar 

  • Stamp JA & Henshaw GG (1987) Somatic embryogenesis from clonal leaf tissue of cassava. Ann. Bot. 59: 445–450

    Google Scholar 

  • Sudarmonowati E & Henshaw GG (1993) The induction of somatic embryogenesis of recalcitrant cassava cultivars using Picloram and Dicamba. In: Proceeding of the First International Scientific Meeting of the Cassava Biotechnology Network (CBN), (pp 128–133). 25-28 August 1992. Cartagena, Colombia

    Google Scholar 

  • Szabados L, Hoyos R & Roca W (1987) In vitro somatic embryogenesis and plant regeneration of cassava. Plant Cell Rep. 6: 248–251

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. South China Institute of Botany, The Chinese Academy of Sciences, Guangzhou, 510650, China

    Guohua Ma & Qiusheng Xu

Authors
  1. Guohua Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiusheng Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guohua Ma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ma, G., Xu, Q. Induction of somatic embryogenesis and adventitious shoots from immature leaves of cassava. Plant Cell, Tissue and Organ Culture 70, 281–288 (2002). https://doi.org/10.1023/A:1016569617969

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016569617969

Search

Navigation