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The effect of NaCl on proline accumulation in rice leaves

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Abstract

The regulation of proline accumulation in detached leaves of rice(Oryza sativa cv. Taichung Native 1) was investigated.Increasing concentrations of NaCl from 50 to 200 mM progressivelyincreased proline content in detached rice leaves. NaCl induced prolineaccumulation was mainly due to the effect of both Na+ andCl− ions. Proline accumulation caused by NaCl was related toprotein proteolysis, an increase in ornithine-δ-aminotransferaseactivity,a decrease in proline dehydrogenase activity, a decrease in prolineutilisation,and an increase in the content of the precursors of proline biosynthesis,ornithine and arginine. Results also show that proline accumulation caused byNaCl was associated with ammonium ion accumulation.

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References

  • Bates L.S., Waldren S.P. and Teare I.D. 1973. Rapid determination of free proline for water-stress studies. Plant Soil. 39: 205–207.

    Google Scholar 

  • Bhaskaran S., Smith R.H. and Newton R.J. 1985. Physiological changes in cultured sorghum cells in response to induced water stress I. Free proline. Plant Physiol. 79: 266–269.

    Google Scholar 

  • Boyer J.S. 1982. Plant productivity and environment. Science 218: 443–448.

    Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Google Scholar 

  • Brown D.H. and Fowden L. 1966. Metabolism of δ-acetylornithine in two Leguminous species. Phytochemistry 5: 887–892.

    Google Scholar 

  • Charest C. and Phan C.T. 1990. Cold acclimation of wheat (Triticum aestivum): Properties of enzymes involved in proline metabolism. Physiol. Plant. 80: 159–168.

    Google Scholar 

  • Chiang H.-H. and Dandekar A.M. 1995. Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and in response to desiccation. Plant Cell Environ. 18: 1280–1290.

    Google Scholar 

  • Delauney A.J. and Verma D.P.S. 1993. Proline biosynthesis and osmoregulation in lants. Plant J. 4: 215–223.

    Google Scholar 

  • Fukutaku Y. and Yamada Y. 1984. Sources of proline nitrogen in water-stressed soybean (Glycine max). II. Fate of 15N-labelled protein. Physiol. Plant. 61: 622–628.

    Google Scholar 

  • Garcia A.B., de Almeida J., Iyer S., Gerats T., Van Montagu M. and Caplan A.B. 1997. Effect of osmoprotectants upon NaCl stress in rice. Plant Physiol. 115: 159–169.

    Google Scholar 

  • Hare P.D., Cress W.A. and Van Stadan J. 1999. Proline synthesis and degradation: a model system for eludicating stress-related signal transduction. J. Exp. Bot. 50: 413–434.

    Google Scholar 

  • Hodson M.J., Opik H. and Wainwright S.J. 1985. Changes in ion and water content of individual shoot organs in a salt-tolerant and a salt-sensitive clone of Agrostis stoloniferra L. during and subsequent to treatment with sodium chloride. Plant Cell Environ. 8: 657–658.

    Google Scholar 

  • Hunt J. 1982. Dilute hydrochloric acid extraction of plant material for routine cation analysis. Commun. Soil Sci. Plant Analysis 13: 49–55.

    Google Scholar 

  • Ireland A.J. and Lea P.J. 1999. The enzymes of glutamine, glutamate, aspargine, and aspartate metabolism. In: Singh B.K. (ed.), Plant Amino Acids. Mercel Dekker Inc., New York, pp. 49–109.

    Google Scholar 

  • Johnson C.M., Stout P.R., Broyer T.C. and Carlton A.B. 1957. Comparative choline requirements of different plant species. Plant Soil. 8: 337–353.

    Google Scholar 

  • La Rosa P.C., Rhodes D., Rhodes J.C., Bressan R. and Csonka L.N. 1991. Elevated accumulation of proline in NaCl-adapted tobacco cells is not due to altered Δ1-pyrroline-5-carboxylate reductase. Plant Physiol. 96: 245–250.

    Google Scholar 

  • Lin C.C. and Kao C.H. 1996. Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl. Plant Sci. 114: 121–128.

    Google Scholar 

  • Lin C.C. and Kao C.H. 2001. Regulation of ammonium-induced proline accumulation in detached rice leaves. Plant Growth Regul. 35: 69–74.

    Google Scholar 

  • Lin J.-N. and Kao C.H. 1998. Water stress, ammonium, and leaf senescence in detached rice leaves. Plant Growth Regul. 26: 165–169.

    Google Scholar 

  • Lin J.-N., Wang J.-W. and Kao C.H. 1999. Effect of abscisic acid and water stress on the senescence of detached rice leaves. Biol. Plant. 42: 313–316.

    Google Scholar 

  • Lin W. 1991. Inhibition of anion transport in corn root protoplast. Plant Physiol. 68: 435–438.

    Google Scholar 

  • Lingnowski E.M. and Splittstoesser W.E. 1971. The change in arginine levels and metabolism of urea and ornithine in Cucurbita moschatta seedlings. Physiol. Plant. 25: 225–229.

    Google Scholar 

  • Lutts S., Kinet J.M. and Bouharmont J. 1996.Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. Plant Growth Regul. 91: 207–218.

    Google Scholar 

  • Lutts S., Majerus V. and Kinet J.M. 1999. NaCl effects on proline metabolism in rice (Oryza sativa L.) seedlings. Physiol. Plant. 105: 450–458.

    Google Scholar 

  • Madan S., Nainawatee H.S., Jain R.K. and Chowdhury J.B. 1995. Proline and proline metabolising enzymes in in-vitro selected NaCl-tolerant Brassica juncea L. under salt stress. Ann. Bot. 76: 51–57.

    Google Scholar 

  • Mattioni C., Lacerenza N.G., Troccoli A., De Leonardis A.M. and Di Fonzo N. 1997. Water and salt stress-induced alterations in proline metabolism of Triticum durum seedlings. Physiol. Plant. 101: 787–792.

    Google Scholar 

  • Moftah A.F. and Michel B.E. 1987. The effect of sodium chloride on solute potential and proline accumulation in soybean leaves. Plant Physiol. 83: 238–240.

    Google Scholar 

  • Oaks A., Stulen J., Jones K., Winspear M.J., Misra S. and Boesel I.L. 1980. Enzymes of nitrogen assimilation in maize roots. Planta 148: 477–484.

    Google Scholar 

  • Paleg L.G., Stewart G.R. and Bradbeer J.W. 1984. Proline and glycine betaine influence protein solvation. Plant Physiol. 75: 974–978.

    Google Scholar 

  • Pollard A. and Wyn Jones R.G. 1979. Enzyme activities in concentrated solutions of glycinebetaine and other solutes. Planta 144: 291–298.

    Google Scholar 

  • Rabe E. 1990. Stress physiology: the functional significance of the accumulation of nitrogen-containing compounds. J. Hort. Sci. 65: 231–243.

    Google Scholar 

  • Rhodes D., Verslues P.E. and Sharp R.E. 1999. Role of amino acids in abiotic stress resistance. In: Singh B.K. (ed.), Plant Amino Acids. Mercel Dekker Inc., New York, pp. 319–356.

    Google Scholar 

  • Roosens N.H.C.J., Thu T.T., Iskandar H.M. and Jacobs M. 1998. Isolation of the ornithine-δ-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana. Plant Physiol. 117: 263–271.

    Google Scholar 

  • Schwab K.B. and Gaff D.F. 1990. Influence of compatible solutes on soluble enzymes from desiccation-tolerant Sporobolus stap fianus and desiccation-sensitive Sporobolus pyramidalis. J. Plant Physiol. 137: 208–215.

    Google Scholar 

  • Smirnoff N. and Cumbes Q.J. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28: 1057–1060.

    Google Scholar 

  • Sudhakar C., Reddy P.S. and Veeranjaneyulu K. 1993. Effect of salt stress on the enzymes of proline synthesis and oxidation in greengram (Phaseolus aureus Roxb.) seedlings. J, Plant Physiol. 141: 621–623.

    Google Scholar 

  • Treichel S. 1986. The influence on Δ1-pyrroline-5-carboxylate reductase in proline-accumulating cell expansion cultures of Mesembryanthemum nodiflorum and other halophytes. Physiol. Plant. 67: 173–181.

    Google Scholar 

  • Tully R.E., Hanson A.D. and Nelsen C.H.E. 1979. Proline accumulation in water-stressed barley leaves in relation to translocation and the nitrogen-budget. Plant Physiol. 63: 518–523.

    Google Scholar 

  • Vogel R.H. and Kopac M.J. 1960. Some properties of ornithine-δ-transaminase from Neurospora. Biochem. Biphys. Acta 37: 539–540.

    Google Scholar 

  • Weatherley P.F. 1950. Studies in the water relation of cotton plant I. The field measurement of water deficits in leaves. New Phytol. 49: 81–97.

    Google Scholar 

  • Yang C.-W. and Kao C.H. 1999. Ammonium in relation to proline accumulation in detached rice leaves. Plant Growth Regul. 30: 139–144.

    Google Scholar 

  • Yang C.-W., Lin C.C. and Kao C.H. 1999. Endogenous ornithine and arginine contents and dark-induced proline accumulation in detached rice leaves. J. Plant Physiol. 155: 665–668.

    Google Scholar 

  • Yang C.-W., Wang J.-W. and Kao C.H. 2000. The relation between accumulation of abscisic acid and proline in detached rice leaves. Biol. Plant. 43: 301–304.

    Google Scholar 

  • Yoshiba Y., Kiyosue T., Nakashima K., Yamaguchi-Shinozaki K. and Shinozaki K. 1997. Regulation of levels of proline as an osmolyte in plants under water stress. Plant Cell Physiol. 38: 1095–1102.

    Google Scholar 

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  1. Department of Agronomy, National Taiwan University, Taipei, Republic of China

    Chuan Chi Lin, Yi Ting Hsu & Ching Huei Kao

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  1. Chuan Chi Lin
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  2. Yi Ting Hsu
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  3. Ching Huei Kao
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Correspondence to Ching Huei Kao.

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Chi Lin, C., Ting Hsu, Y. & Huei Kao, C. The effect of NaCl on proline accumulation in rice leaves. Plant Growth Regulation 36, 275–285 (2002). https://doi.org/10.1023/A:1016518013449

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