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Amiloride: Biological Fluid Analysis by Reverse-Phase HPLC

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Abstract

Amiloride is an effective anti-kaliuretic-diuretic which has been used clinically since 1965. Because of a lack of sensitivity in reported analytical methods, useful pharmacokinetic data have been sparse. This publication describes a sensitive (1 ng/ml), rapid, and reproducible reverse-phase HPLC technique for the quantitative measurement of amiloride levels in both serum and urine specimens. Sample clean-up is based on the adsorption of amiloride to commercially available silica cartridges and its selective elution with perchloric acid. The eluted drug is further resolved from endogenous interferences on a Waters' C-18 µBondapak analytical column with a mobile phase consisting of methanol:sodium perchlorate (0.1 M, pH 4.0) 40:60 v/v and fluorescence detection (λex = 368 nm, λem = 417 nm). This method has been applied to the analysis of human serum and urine samples.

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Authors and Affiliations

  1. Merck Sharp and Dohme Research Laboratories, West Point, PA

    Gene A. Hessey II, Marvin L. Constanzer & William F. Bayne

  2. Smith Kline & French Laboratories, Philadelphia, PA, 19101

    William C. Vincek

  3. MSDRL, W26-372

    Gene A. Hessey II

Authors
  1. William C. Vincek
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  2. Gene A. Hessey II
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  3. Marvin L. Constanzer
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  4. William F. Bayne
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Vincek, W.C., Hessey II, G.A., Constanzer, M.L. et al. Amiloride: Biological Fluid Analysis by Reverse-Phase HPLC. Pharm Res 2, 143–145 (1985). https://doi.org/10.1023/A:1016371618594

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  • DOI: https://doi.org/10.1023/A:1016371618594

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