, Volume 126, Issue 2, pp 169–176 | Cite as

Assessment of genetic variability in grapefruits (Citrus paradisi Macf.) and pummelos (C. maxima (Burm.) Merr.) using RAPD and SSR markers

  • M.J. Corazza-Nunes
  • M.A. Machado
  • W.M.C. Nunes
  • M. Cristofani
  • M.L.P.N. Targon


The genetic variability of 38 grapefruit (Citrus paradisi Macf.) and three pummelos (C. maxima (Burm.) Merr..) accessions was evaluated using RAPD, and single sequence repeat (SSR) analyses. Approximately49% of the 198 RAPD were polymorphic, and 4.6 alleles per SSR loci were identified. PIC values changed from 0.093 to 0.450. A UPGMA phenetic tree was constructed and two main grapefruit groups were identified. The grapefruit accessions `do Cabo' and `Siamesa-Filipinas'clustered very close to the pummelos in Group A. The Group B consisted of three sub-groups, which comprised all of the other grapefruit accessions. The majority of grapefruit accessions showed a narrow genetic base suggesting that the observed morphological polymorphism within the group must be associated with somatic mutations, which were not detected by these molecular markers.

Citrus paradisi grapefruits RAPD markers SSR markers 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • M.J. Corazza-Nunes
    • 1
  • M.A. Machado
    • 2
  • W.M.C. Nunes
    • 3
  • M. Cristofani
    • 2
  • M.L.P.N. Targon
    • 2
  1. 1.Departamento de BiologiaUniversidade Estadual de MaringáMaringá-PRBrazil
  2. 2.Centro de Citricultura Sylvio Moreira/IACCordeirópolis-SPBrazil
  3. 3.Departamento de AgronomiaUniversidade Estadual de MaringáMaringá-PRBrazil

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