Abstract
Biochemical consequences of mutational damage to common components of the Erwinia phosphoenolpyruvate-dependent phosphotransferase system (the HPr protein and enzyme I) were studied. The transport of glucose, mannose, fructose, and mannitol inErwinia was shown to require a preliminary induction of proteins of the phosphotransferase system. A drastic decrease in the rate of the transport of these carbohydrates was observed in ptsI and ptsH mutants. A disturbance in the common components suppresses the synthesis of inducible enzymes (β-galactosidase, complexes of pectolate lyases and cellulases) and renders it resistant to catabolite repression by glucose, but mutants were shown to retain intracellular cAMP content. Erwinia mutants devoid of common components of the system lack phytopathogenic features. The appearance of an intact ptsI allele in the cell completely repaired pleiotropic disturbances in these mutants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Postma, P.W., Lengeler, J.W., and Jacobson, G.R., Phosphoenolpyruvate: Carbohydrate Phosphotransferase Systems of Bacteria, Microbiol. Rev., 1993, vol. 57, pp. 543-594.
Gershanovitch, V.N., Bolshakova, T.N., Erlagaeva, R.S., et al., Phosphoenolpyruvate-Dependent System and Metabolic Regulation in the Bacterial Cell, Usp. Sovrem. Biol., 1987, vol. 103, pp. 173-188.
Bolshakova, T.N., Gabrielyan, T.R., Bourd, G.I., and Gershanovitch, V.N., Involvement of the Escherichia coli Phosphoenolpyruvate-Dependent Phosphotransferase System in Regulation of Transcription of Catabolic Genes, Eur. J. Biochem., 1978, vol. 89, pp. 483-490.
Gershanovitch, V.N., Bolshakova, T.N., Molchanova, M.L., et al., Fructose-Specific Phosphoenolpyruvate-Dependent Phosphotransferase System of Escherichia coli: Its Alterations and Adenylate Cyclase Activity, FEMS Microbiol. Rev., 1989, vol. 63, pp. 125-134.
Stulke, J., Arnaud, M., Rapoport, G., and Martin-Verstraete, I., PRD-A Protein Domain Involved in PTS-Dependent Induction and Carbon Catabolite Repression of Catabolic Operons in Bacteria, Mol. Microbiol., 1998, vol. 28, pp. 865-874.
Stulke, J. and Hillen, W., Coupling Physiology and Gene Regulation in Bacteria: The Phosphotransferase System Delivers the Signals, Natur. Wissenschaften, 1998, vol. 85, pp. 583-592.
Lycheva, T.A., Bondarenko, V.M., Bourd, G.I., et al., Characterization of Attenuated Immunogenic Shigella flexneri Mutants Selected from Phosphomycin-Resistant Clones, Zh. Mikrobiol., Epidemiol. Immunol., 1980, vol. 6, pp. 58-63.
Gershanovitch, V.N., Umyarov, A.M., Bourd, G.I., et al., A Shigella flexneri Mutation Causing Generation of Phosphomycin-Resistant Avirulent Forms with Disturbed Carbohydrate Utilization, Zh. Mikrobiol., Epidemiol. Immunol., 1980, vol. 11, pp. 83-88.
Hugouvieux-Cotte-Pattat, N., Condemine, G., Nasser, W., and Reverchon, S., Regulation of Pectinolysis in Erwinia chrysanthemi, Annu. Rev. Microbiol., 1996, vol. 50, pp. 213-257.
Evtushenkov, A.N., Prokulevich, V.A., Belyasova, N.A., et al., Erwinia chrysanthemi Mutants Defective in Secretion of Extracellular Pectate Lyases, Mol. Genet., Mikrobiol. Virusol., 1984, vol. 8, pp. 26-30.
Evtushenkov, A.N. and Fomichev, Yu.K., Secretion of Pectate Lyases by Erwinia chrysanthemi and Erwinia carotovora var. atroseptica Cells, Mikrobiologiya, 1996, vol. 65, pp. 333-338.
Prokulevich, V.A., Organization of the Erwinia chrysanthemi Genome, Doctoral (Biol.) Dissertation, Moscow: All-Union Research Institute of Genetics and Selection of Industrial Miicroorganisms, 1989.
Nikolaichik, E.A. and Pesnyakevich, A.G., A Circular Genetic Map of the Chromosome of Erwinia carotovora subsp. atroseptica 3-2, Genetika (Moscow), 1995, vol. 31, no. 8, pp. 1052-1058.
Datsenko, K.A., Evtushenkov, A.N., and Bolshakova, T.N., Isolation and Genetic Characterization of Erwinia Mutants Devoid of the Common Components of the Phosphoenolpyruvate-Dependent Phosphotransferase System, Genetika (Moscow), 2002, vol. 38, no. 5, pp. 622-628.
Miller, J.H., Experiments in Molecular Genetics, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1972.
Bolshakova, T.N., Bobrynina, O.Yu., and Gershanovitch, V.N., Isolation and Investigation of the Escherichia coli Mutant with the Deletion in the ptsH Gene, FEBS Lett., 1979, vol. 107, pp. 169-172.
James, V. and Hugouveux-Cotte-Pattat, N., Regulatory Systems Modulating the Transcription of the Pectinase Genes of Erwinia chrysanthemi Are Conserved in Escherichia coli, Microbiology, 1996, vol. 142, pp. 2613-2619.
Revershon, S., Expert, D., Robert-Baudouy, J., and Nassar, W., The Cyclic AMP Receptor Protein Is the Main Activator of Pectinolysis Genes in Erwinia chrysanthemi, J. Bacteriol., 1997, vol. 179, pp. 3500-3508.
Glesyna, M.L., Bolshakova, T.N., and Gershanovitch, V.N., Effect of ptsI and ptsH Mutations on Initiation of Transcription of the Escherichia coli Lactose Operon, Mol. Gen. Genet., 1983, vol. 107, pp. 169-172.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Datsenko, K.A., Evtushenkov, A.N., Sergeev, K.V. et al. Properties of Mutants of Bacteria Belonging the Genus Erwinia Devoid of Common Components of the Phosphoenolpyruvate-Dependent Phosphotransferase System. Russian Journal of Genetics 38, 754–760 (2002). https://doi.org/10.1023/A:1016331403100
Issue Date:
DOI: https://doi.org/10.1023/A:1016331403100