Abstract
Using preparative isoelectric focusing, fast performance liquid chromatography, and polyacrylamide gel electrophoresis accumulation of several Al-induced cytoplasmic proteins was described in barley roots. Two of them, 27 and 28 kDa polypeptides were isolated by continuous-elution electrophoresis system in sufficient quantities for their further characterisation.
References
Aniol, A.: Induction of aluminum tolerance in wheat seedlings by low doses of aluminum in the nutrient solution.-Plant Physiol. 75: 551–555, 1984.
Basu, A., Basu, U., Taylor, G.J.: Induction of microsomal membrane proteins in roots of an aluminum-resistant cultivar of Triticum aestivum L. under condition of aluminum stress.-Plant Physiol. 104: 1007–1013, 1994.
Delhaize, E., Ryan, P.R.: Aluminum toxicity and tolerance in plants.-Plant Physiol. 107: 315–321, 1995.
Kochian, L.V.: Cellular mechanisms of aluminum toxicity and resisatnce in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 46: 237–260, 1995.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4.-Nature 277: 680–685, 1970.
Matsumoto, H.: Cell biology of aluminum toxicity and tolerance in higher plants.-Int. Rev. Cytol. 200: 1–46, 2000.
Ownby, J.D., Hruschka, W.R.: Quantitative changes in cytoplasmic and microsomal proteins associated with aluminium toxicity in two cultivars of winter wheat.-Plant Cell Environ. 14: 303–309, 1991.
Tamás, L., Mistrík, I., Huttová, J.: Protein profiles in roots of aluminium sensitive and resistant barley cultivars after aluminium treatment.-Biologia (Bratislava) 54: 459–465, 1999.
Uexküll, H.R. von, Mutert, E.: Global extent, development and economic impact of acid soils.-Plant Soil 171: 1–15, 1995.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tamás, L., Mistrík, I. & Huttová, J. Isolation and Partial Characterization of Aluminium-Induced Cytoplasmic Polypeptides from Barley Root. Biologia Plantarum 45, 463–467 (2002). https://doi.org/10.1023/A:1016294323269
Issue Date:
DOI: https://doi.org/10.1023/A:1016294323269