Abstract
With the aim to determine the effects of CO2 on nitrogen metabolism mungbean (Vigna radiata) plants were grown from seedling emergence to maturity inside open top chambers under ambient CO2 (CA, 350 ± 25 µmol mol−1) and elevated CO2 (CE, 600 ± 50 µmol mol−1) concentrations at the Indian Agricultural Research Institute, New Delhi. Leaflet blades of the same physiological age were sampled at 20, 35 and 50 d after germination. Total nitrogen concentration in dry mass was consistently lower under CE than in CA. Non-protein nitrogen and protein nitrogen were also decreased under CE Total soluble protein content also decreased up to 35 d after germination under CE. However, a 27 % increase in protein content at 50 d after germination due to CE was observed. A significant decrease in total free amino acid under CE at 20 d after germination was observed. CE also brought about a remarkable decrease in the activity of nitrate reductase in leaves at 20 d after germination but increase at 35 d and 50 d after germination. Nitrogenase activity increased at all growth stages due to CE. Although total harvested leaves of CE plants accumulated more nitrogen, the relative amount of nitrogen on a percentage basis was low, probably due to a comparatively greater accumulation of sugars in the leaves of CE plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen, L.H., Jr., Vu, J.C.V., Valle, R.R., Botte, K.J., Jones, P.H.: Non-structural carbohydrates and nitrogen of soybean grown under CO2 enrichment.-Crop Sci. 28: 84–94, 1988.
Cramer, M.D., Savidov, N.A., Lips, S.H.: The influence of enriched rhizosphere CO2 on nitrogen uptake and metabolism in wild type and NR deficient barley plants.-Physiol. Plant. 97: 47–54, 1996.
Evans, J.R.: Photosynthesis and nitrogen relationships in leaves of C3 plants.-Oecologia 78: 9–19, 1989.
Finn, G.A., Brun, W.A.: Effects of atmospheric CO2 enrichment on growth, non-structural carbohydrate content and root nodule activity in soybean.-Plant Physiol. 69: 327–331, 1982.
Gifford, R.M., Lambers, H., James, I., Morrison, L.: Respiration of crop species under CO2 enrichment.-Physiol. Plant. 63: 351–356, 1985.
Goudriaan, J., De Ruiter, H.E.: Plant growth in response to CO2 enrichment at two levels of nitrogen and phosphorus supply I. Dry matter, leaf area and development.-Neth J. agr. Sci. 31: 157–169, 1983.
Griffin, K.L., Luo, Y.: Sensitivity and acclimation of Glycine max (L.) Merr. leaf gas exchange to CO2 partial pressure.-Environ. exp. Bot. 42: 141–153, 1999.
Hardy, R.W.F., Holsten, R.D., Jackson, E.K., Burns, R.C.: The acetylene reduction assay for nitrogen fixation: laboratory and field evaluation.-Plant Physiol. 43: 1195–1207, 1968.
Hocking, P.J., Meyer, C.P.: Responses of noogoora burr (Xanthium occidentale Bertol) to nitrogen supply and CO2 enrichment.-Ann. Bot. 55: 835–894, 1985.
Hocking, P.J., Meyer, C.P.: Effects of CO2 enrichment and nitrogen stress on growth, and partitioning of dry matter and nitrogen in wheat and maize.-Aust. J. Plant Physiol. 18: 339–356, 1991.
Jones, P., Allen, L.H., Jones, J.W., Valle, R.: Photosynthesis and transpiration responses of soybean canopies to short and long term CO2 treatments.-Agron. J. 77: 119–126, 1985.
Klepper, L., Flesher, D., Hageman, R.H.: Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves.-Plant Physiol. 48: 580–585, 1971.
Larigauderie, A., Hilbert, D.W., Oechel, W.C.: Effect of CO2 enrichment and nitrogen availability on resource acquisition and resource allocation in a grass, Bromus mollis.-Oecologia 77: 544–549, 1988.
Lee, Y.P., Takahashi, T.: An improved colorimetric determination of amino acid with the use of ninhydrin.-Ann. Bot. 12: 71–77, 1966.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.
Manderscheid, R., Bender, J., Jager, H.J., Weigel, H.J.: Effects of season long CO2 enrichment on cereals. II. Nutrient concentrations and grain quality.-Agr. Ecosyst. Environ. 54: 175–185, 1995.
Madore, M., Grodzinski, B.: Effects of CO2 enrichment on growth and photoassimilate transport in dwarf cucumber (Cucumis sativus L.) cultivar.-J. Plant Physiol. 121: 59–71, 1985.
Masterson, C.L., Sherwood, M.T.: Some effects of increased atmospheric carbon dioxide on white clover (Trifolium repens) and pea (Pisum sativum).-Plant Soil 49: 421–426, 1978.
McKee, I.F., Woodward, F.I.: CO2 enrichment responses of wheat: Interactions with temperature, nitrate and phosphate.-New Phytol. 127: 447–453, 1994.
Mjwara, J.M., Botha, C.E.J., Radloff, S.E.: Photosynthesis, growth and nutrient changes in non nodulated Phaseolus vulgaris grown under atmospheric and elevated CO2 conditions.-Physiol. Plant. 97: 754–763, 1996.
Panse, V.G., Sukhatme, P.T. (ed.): Statistical Methods for Agricultural Workers.-Indian Council of Agricultural Research, New Delhi 1967.
Reeves, D.W., Rogers, H.H., Prior, S.A., Wood, C.W., Runion, G.B.: Elevated atmospheric CO2 effects on sorghum and soybean nutrient status.-J. Plant Nutr. 17: 1939–1954, 1994.
Rogers, H.H., Heck, W.W., Heagle, A.S.: A field techniques for the study of plant responses to elevated CO2 concentration.-Air Pollution Control Assoc. J. 33: 42–44, 1983.
Sage, R.F.: Acclimation of photosynthesis to increasing atmospheric CO2: the gas exchange perspective.-Photosynth. Res. 39: 351–368, 1994.
Schmitt, M.R., Edwards, G.E.: Photosynthetic capacity and nitrogen use efficiency of maize, wheat and rice: a comparison between C3 and C4 photosynthesis.-J. exp. Bot. 32: 459–466, 1981.
Sharma, A., Sengupta, U.K.: CO2 enrichment effects on photosynthesis and related enzymes in Vigna radiata.-Indian J. Plant Physiol. 33: 340–346, 1990.
Ulman, P., Čatský, J., Pospíšilová, J.: Photosynthetic traits in wheat grown under decreased and increased CO2 concentration, and after transfer to natural CO2 concentration.-Biol. Plant. 43: 227–237, 2000.
Uprety, D.C., Rabha, B.K.: Effect of elevated CO2 and moisture stress on the carbon and nitrogen contents in Brassica juncea.-Biol. Plant. 42: 133–136, 1999.
Vu, J.C.V., Allen, L.H., Jr., Bowes, G.: Leaf ultrastructure, carbohydrates and protein of soybean grown under CO2 enrichment.-Environ. exp. Bot. 29: 141–147, 1989.
Vu, J.C.V., Allen, L.H., Jr., Bowes, G.: Effects of elevated CO2 and temperature on photosynthesis and Rubisco in rice and soybean.-Plant Cell Environ. 20: 68–76, 1997.
Warrick, R.A., Gifford, R.M.: CO2 climatic change and agriculture; assessing the response of food crops to the direct effects of increased CO2 and climatic change.-In: Bolin, B., Doos, B., Jager, J., Warrick, R.A. (ed.): SCOPE 29. The Greenhouse Effect, Climatic Change and Ecosystems. Pp 393–474. John Wiley & Sons, Chichester 1986.
Wheeler, C.T., Cameron, E.M., Gordon, J.C.: Effects of handling and surgical treatments on nitrogenase activity in root nodules of Alnus glutinosa, with special reference to the application of indole-acetic acid.-New Phytol 80: 175–178, 1978.
Wilkins, D., Van Oosten J.J., Besford, R.T., Scarascia, M.G.E.: Effects of elevated CO2 on growth and chloroplast proteins in Prunus avium.-Tree Physiol 14: 769–779, 1994.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Srivastava, A., Pal, M. & Sengupta, U. Changes in Nitrogen Metabolism of Vigna Radiata in Response to Elevated CO2 . Biologia Plantarum 45, 395–399 (2002). https://doi.org/10.1023/A:1016269717817
Issue Date:
DOI: https://doi.org/10.1023/A:1016269717817