Abstract
The cloning of a 465 bp fragment from the 5′-flanking region of the gene encoding a cytosolic cyclophilin from periwinkle was achieved through inverse polymerase chain reaction. The DNA fragment was fused to a gusA-intron marker then introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Histochemical analysis of the transgenic shoot cultures demonstrated that the construct was able to drive GUS expression in stomata guard cells, but not in mesophyll cells when shoots were still attached to the callus from which they were initiated. In separated transgenic shoots and in seedlings, GUS was expressed in external and internal phloem and root hairs, respectively. GUS activity in transgenic tobacco seedlings was also investigated by fluorimetric assays. Treatments with NaCl or ABA decreased promoter activity whereas treatment with yeast extracts increased it.
Similar content being viewed by others
References
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Chou, I.T., Gasser, C.S.: Characterization of the cyclophilin gene family of Arabidopsis thaliana and phylogenetic analysis of known cyclophilin proteins.-Plant mol. Biol. 35: 873–892, 1997.
Clastre, M., Maaroufi, H., Andreu, F., Chénieux, J.C., Rideau, M., Hamdi, S.: Isolation of a full-length cDNA encoding a cytosolic cyclophilin from periwinkle (Catharanthus roseus).-Plant Physiol. 109: 1497, 1995.
Deblaere, R., Bytebier, B., De Greve, H., Deboeck, F., Schell, J., Van Montagu, M., Leemans, J.: Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.-Nucl. Acids Res. 13: 4777–4788, 1985.
Deng, W., Chen, L., Wood, D.W., Metcalfe, T., Liang, X., Gordon, M.P., Comai, L., Nester, E.W.: Agrobacterium VirD2 protein interacts with plant host cyclophilins.-Proc. nat. Acad. Sci. USA 95: 7040–7045, 1998.
Doyle, V., Virji, S., Crompton, M.: Evidence that cyclophilin-A protects cells against oxidative stress.-Biochem. J. 341: 127–132, 1999.
Droual, A.M., Maaroufi, H., Crèche, J., Chénieux, J.C., Rideau, M., Hamdi, S.: Changes in the accumulation of cytosolic cyclophilin transcripts in cultured periwinkle cells following hormonal and stress treatments.-J. Plant Physiol. 151: 142–150, 1997.
Duina, A.A., Marsh, J.A., Gaber, R.F.: Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth.-Yeast 12: 943–952, 1996.
Ferreira, P.A., Nakayama, T.A., Pak, W.L., Travis, G.H.: Cyclophilin-related protein RanBP2 acts as chaperonne for red/green opsin.-Nature 383: 637–640, 1996.
Franke, E.K., Yuan, H.E., Luban, J.: Specific incorporation of cyclophilin A into HIV-I virions.-Nature 372: 359–362, 1994.
Fulgosi, H., Vener, A.V., Altschmied, L., Herrmann, R.G., Andersson, B.: A novel multi-functional chloroplast protein: identification of a 40 kDa immunophilin-like protein located in the thylakoid lumen.-EMBO J. 17: 1577–1587, 1998.
Gasser, C.S., Gunning, D.A., Budelier, K.A., Brown, S.M.: Structure and expression of cytosolic cyclophilin/peptidylprolyl cis-trans isomerase of higher plants and production of active tomato cyclophilin in Escherichia coli.-Proc. nat. Acad. Sci. USA 87: 9519–9523, 1990.
Godoy, A.V., Lazzaro, A.S., Casalongué, C.A., San Segundo, B.: Expression of a Solanum tuberosum cyclophilin gene is regulated by fungal infection and abiotic stress conditions.-Plant Sci. 152: 123–134, 2000.
Horsch, R.B., Rogers, S.G., Fraley, R.T.: Transgenic plants.-Cold Spring Harb. Symp. quant. Biol. 50: 433–437, 1985.
Jackson, K., Soll, D.: Mutations in a new Arabidopsis cyclophilin disrupt its interaction with protein phosphatase 2A.-Mol. gen. Genet. 262: 830–838, 1999.
Jin, Z.G., Melaragno, M.G., Liao, D.F., Yan, C., Haendeler, J., Suh, Y.A., Lambeth, J.D., Berk, B.C.: Cyclophilin A is a secreted growth factor induced by oxidative stress.-Circ. Res. 87: 789–796, 2000.
Krummrei, U., Bang, R., Schmidtchen, R., Brune, K., Bang, H.: Cyclophilin-A is a zinc-dependent DNA binding protein in macrophages.-FEBS Lett. 371: 47–54, 1995.
Kullertz, G., Liebau, A., Rucknagel, P., Schierhorn, A., Diettrich, B., Fischer, G., Luckner, M.: Stress-induced expression of cyclophilins in proembryonic masses of Digitalis lanata does not protect against freezing/thawing stress.-Planta 208: 599–605, 1999.
Luan, S., Li, W., Rusnak, F., Assmann, S.M., Schreiber, S.L.: Immunosuppressants implicate protein phosphatase regulation of K+ channels in guard cells.-Proc. nat. Acad. Sci. USA 90: 2202–2206, 1993.
Marivet, J., Frendo, P., Burkard, G.: Effects of abiotic stresses on cyclophilin gene expression in maize and bean, and sequence analysis of bean cyclophilin cDNA.-Plant Sci. 84: 171–178, 1992.
Marivet, J., Frendo, P., Burkard, G.: DNA sequence analysis of a cyclophilin gene from maize: developmental expression and regulation by salicyclic acid.-Mol. gen. Genet. 247: 222–228, 1995.
Marivet, J., Margis-Pinheiro, M., Frendo, P., Burkard, G.: Bean cyclophilin gene expression during plant development and stress conditions.-Plant mol. Biol. 26: 1181–1189, 1994.
Marty, I., Brudigou, C., Chartier, Y., Meyer, Y.: Growth-related gene expression in Nicotiana tabacum mesophyll protoplasts.-Plant J. 4: 265–278, 1993.
Montague, J.W., Hughes, F.M., Cidlowski, J.A.: Native recombinant cyclophilins A, B, and C degrade DNA independently of peptidylprolyl cis-trans-isomerase activity. Potential roles of cyclophilins in apoptosis.-J. biol. Chem. 272: 6677–6684, 1997.
Saito, T., Tadakuma, K., Takahashi, N., Ashida, H., Tanaka, K., Kawamukai, M., Matsuda, H., Nakagawa, T.: Two cytosolic cyclophilin genes of Arabidopsis thaliana differently regulated in temporal-and organ-specific expression.-Biosci. Biotechnol. Biochem. 63: 632–637, 1999.
Santos, A.N., Korber, S., Kullertz, G., Fischer, G., Fischer, B.: Oxygen stress increases prolyl cis/trans isomerase activity and expression of cyclophilin 18 in rabbit blastocysts.-Biol. Reprod. 62: 1–7, 2001.
Scholze, C., Peterson, A., Diettrich, B., Luckner, M.: Cyclophilin isoforms from Digitalis lanata. Sequences and expression during embryogenesis and stress.-J. Plant Physiol. 15: 212–219, 1999.
Sykes, K., Gething, M.J., Sambrook, J.: Proline isomerases function during heat shock.-Proc. nat. Acad. Sci. USA 90: 5853–5857, 1993.
Yang, W.M., Inouye, C.J., Seto, E.: Cyclophilin A and FKBP12 interact with YY1 and alter its transcriptional activity.-J. biol. Chem. 270: 15187–15193, 1995.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Droual, AM., Creche, J., Andreu, F. et al. The Expression of a Cytosolic Cyclophilin Promoter from Periwinkle in Transgenic Tobacco Plants. Biologia Plantarum 45, 321–326 (2002). https://doi.org/10.1023/A:1016249113274
Issue Date:
DOI: https://doi.org/10.1023/A:1016249113274