Abstract
Callus induction was obtained on Murashige and Skogg agar medium with 45 μM 2,4-dichlorophenoxyacetic acid under dark at 25°C. Among the four explant types investigated, the best callus induction was obtained from two-week old fronds to which a surgical incision was applied in the basal (meristematic) region. This treatment resulted in 89.11% of fronds producing callus which continued to proliferate for another 24 months. To obtain plant regeneration pieces of calluses were transferred onto Murashige and Skoog agar medium containing 22 μM indole-3-acetic acid and 4.6 μM kinetin and maintained under 16-h photoperiod (irradiance of 30 μmol m−2 s−1) at 23°C. Green fronds formed on all callus pieces. The regenerated fronds were later transferred onto Wang medium where they formed roots. The regenerated Lemna minor L. plants obtained through indirect organogenesis did not differ morphologically from individuals forming the stock collection.
References
Chang, W.C., Chiu, P. L.: Regeneration of Lemna gibba G3 through callus culture.-Z. Pflanzenphysiol. 89: 91–94, 1978.
Frick, H.: Callogenesis and carbohydrate utilization in Lemna minor.-J. Plant Physiol. 137: 397–401, 1991.
Frick, H., Morley, K.: Metabolism of lactose by Lemna minor L. (duckweed) callus.-Process Biochem. 30: 57–62, 1995.
Landolt, E.: The Family of Lemnaceae — a Monographic Study. Vol. 1. Zurich, Switzerland.-Veröff. geobot. Inst. E.T.H., Stiftung Rübel 1986.
Moon, H.K., Stomp, A.M.: Effects of medium components and light on callus induction, growth, and frond regeneration in Lemna gibba (duckweed).-In Vitro cell. dev. Biol. Plant 33: 20–25, 1997.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacoo tissue cultures.-Physiol. Plant. 15: 473–496, 1962.
Samardakiewicz, S., Woźny, A.: The distribution of lead in duckweed (Lemna minor L.) root tip.-Plant Soil 226: 107–111, 2000.
Schenk, R.U., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199–204, 1972.
Severi, A., Fornasiero, B.: Morphological variations in Lemna minor L. and possible relationship with abscisic acid.-Caryologia 36: 57–64, 1983.
Wang, W.: Literature review on duckweed toxiticy testing.-Environ. Res. 52: 7–22, 1990.
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Stefaniak, B., Woźny, A. & Budna, I. Callus Induction and Plant Regeneration in Lemna Minor L.. Biologia Plantarum 45, 469–472 (2002). https://doi.org/10.1023/A:1016246507339
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DOI: https://doi.org/10.1023/A:1016246507339