Abstract
Treatment of spruce, fir and oak trees with herbicides, which may be one of the forest damage inducing agents, caused pronounced changes in the contents and distribution of indole-3-acetic acid (IAA) and cytokinins (CKs) one year after treatment, i.e. at the time of the first microscopically visible damage in treated trees. In Picea pungens IAA content increased in the terminal buds by about 105 % and in the apical buds of the first order branches by 220 %. The same was true for young sprouts of Abies nordmanniana, while in leaves of oak trees IAA content was decreased by 15 % after glyphosate treatment and by 30 % after 2,4-dichlorophenoxyacetic acid (2,4-D) treatment. Another striking feature was a significantly decreased content of IAA in the lower parts of roots in Picea pungens (50 % of the control), which is accompanied by an increase in IAA content in the middle part of the roots (130 %). On the other hand, the IAA content of both sprouts and roots of A. nordmanniana was significantly increased after herbicide treatment.
In P. pungens, the content of free cytokinins (sum of zeatin, zeatin riboside, isopentenyladenine and isopentenyladenosine) decreased due to herbicide treatment. The strongest decrease was seen in roots, especially in their upper and middle parts (the average reduction of cytokinin content in roots was 63 %). In the above-ground organs the reduction was seen namely for isopentenyladenine and isopentenyladenosine, while the abundance of zeatin riboside was, on the other hand, higher in treated plants. In Quercus robur leaves, the total content of cytokinins also decreased, namely after glyphosate treatment. In consequence of these changes, CK/IAA ratio decreased pronouncedly in all organs of herbicide-treated trees, with the exception of oak leaves treated by 2,4-D.
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References
Bangerth, F.: Response of cytokinin concentration in the xylem exudate of bean (Phaseolus vulgaris L.) plants to decapitation and auxin treatment, and relationship to apical dominance.-Planta 194: 439–442, 1994.
Börner, H.: Umkrautbekämpfung.-Gustav Fischer Verlag, Jena 1995.
Cline, M.G.: The role of hormones in apical dominance: new approaches to an old problem in plant development.-Physiol. Plant. 90: 230–237, 1994.
Christmann, A., Frenzel, B., Schiller, P.: Phytohormones in needles of healthy and declining silver fir (Abies alba Mill.): II. Abscisci acid.-J. Plant Physiol. 147: 419–425, 1995.
Eder, J., Rovenská, J., Kutáček, M., Čermák, V.: HPLC analysis of indole compounds in Agrobacterium and transformed tobacco cells.-In: Kutáček, M., Bandurski, R.S., Krekule, J. (ed.): Physiology and Biochemistry of Auxins in Plants. Pp. 389–390. Academia, Praha 1988.
El-Hattab, A.H., Hassan, E.A., Shaban, S.A., Abo-Elsuoud, M.R.: Effect of some growth factors and glyphosate herbicide on pigments and protein in faba beans (Vicia faba L.).-J. Agron. Crop Sci. 158: 250–258, 1987.
Hartman, G.: Ursachenanalyse des Eichensterbenss in Deutschland-Versuch einer Synthese bisheriger Befunde.-Mitt. Biol. Bundesanstalt 318: 125–151, 1996.
Hock, B., Elstner, E.F.: Schadwirkungen auf Pflanzen.-Wissenschaftsverlag, Mannheim-Wien-Zürich 1988.
Johnson, J.D.: Stress physiology of forest trees: The role of plant growth regulators.-Plant Growth Regul. 6: 193–215, 1987.
Macháčková, I., Krekule, J., Eder, J., Seidlová, F., Strnad, M.: Cytokinins in photoperiodic induction of flowering in Chenopodium species.-Physiol. Plant. 87: 160–166, 1993.
Matschke, J., Amenda, R.: Wissenswertes über den qualitätsgerechten Anbau von Weihnachtsbäumen.-Weber Netze GmbH, Gudensberg 1998.
Matschke, J., Macháčková, I., Amenda, R.: Hormonveränderungen in belasteten Weihnachtsbaum-Kulturen.-Allgemeine Forstz. 1: 21–24, 1997.
Mohr, H.: Waldschäden in Mitteleuropa — Wo liegen die Ursachen?-Verhandlungen der Gesellschaft Deutscher Naturforscher u. Ärzte 117: 43–59, 1992.
Morris, D.A.: Hormonal regulation of source-sink relationships: an overview of potential control mechanisms.-In: Zamski, E., Schaffer, A.A. (ed.): Photoassimilate Distribution in Plants and Crops. Source-Sink Relationships. Pp. 441–466. Marcel Dekker, New York-Basel-Hong Kong 1996.
Mothes, K.: The role of kinetin in plant regulation.-Colloq. int. Centre nat. Rech. sci. Paris 123: 131–140, 1964.
Mothes, K.: Zur Problematik der Phytokinin-Wirkung.-Biol. Rundschau 4: 221–224, 1996.
Mothes, K., Parthier, B.: Das Altern der Pflanzen und ihrer Organe.-Wissenschaft Fortschritt 34: 40–43, 1984.
Ortiz, R., Vuylsteke, D.R.: Genetics of apical dominance in plantain (Musa spp., AAB Group) and improvement of suchering behavior.-J. amer. Soc. hort. Sci. 119: 1050–1053, 1994.
Rehfuss, K.E.: Was kann Düngung bei den "neuartigen" Walderkrankungen leisten?-AFZ 20: 1090–1093, 1995.
Sachs, T.: Pattern Formation in Plants.-Cambridge Univ. Press, Cambridge 1991.
Steinrücken, H.C., Amrhein, N.: The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase.-Biochem. biophys. Res. Commun. 94: 1207–1212, 1980.
Štiller, J., Našinec, V., Svoboda, S., Němcová, B., Macháčková, I.: Effects of agrobacterial oncogenes in kidney vetch (Anthyllis vulneraria L.).-Plant Cell Rep. 11: 363–367, 1992.
Strnad, M., Vaněk, T., Binarová, P., Kamínek, M., Hanuš, J.: Enzyme immunoassays for cytokinins and their use for immunodetection of cytokinins in alfalfa cell culture.-In: Kutáček, M., Elliott, M.C., Macháčková, I. (ed.): Molecular Aspects of Hormonal Regulation of Plant Development. Pp. 88–95. SPB Acad. Publ., The Hague 1990.
Strnad, M., Peters, W., Beck, E., Kamínek, M.: Immunodetection and identification of N6-(o-hydroxybenzylamino)purine as a naturally occurring cytokinin in Populus × canadensis Moench, cv. Robusta leaves.-Plant Physiol. 99: 74–80, 1992.
Völckers, P., Wild, A.: A specific radioimmunoassay for the determination of low quantities of indole-3-acetic acid in spruce needles of healthy and damaged trees.-J. Plant Physiol. 133: 320–324, 1988.
Von Schwartzenberg, K., Lutze, K., Hahn, H.: Determination of cytokinins in needles of Norway spruce (Picea abies Karst.) by an indirect enzyme-linked immunosorbent assay.-J. Plant Physiol. 133: 529–534, 1988.
Wessler, A., Wild, A.: Investigations on the content of indole-3-acetic acid in spruce needles of healthy and damaged trees of various sites.-J. Plant Physiol. 141: 615–620, 1993.
Wentzel, K.F.: Plausibilität oder Kausalität?-AFZ 17: 921–927, 1992.
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Matschke, J., Macháčková, I. Changes in the Content of Indole-3-Acetic Acid and Cytokinins in Spruce, Fir and Oak Trees after Herbicide Treatment. Biologia Plantarum 45, 375–382 (2002). https://doi.org/10.1023/A:1016213500070
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DOI: https://doi.org/10.1023/A:1016213500070