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Direct Differentiation of Shoot Buds from Leaf Explants of Cajanus Cajan L.

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Biologia Plantarum

Abstract

A protocol was developed for direct differentiation of multiple shoot buds from leaf explants of Cajanus cajan. In a modified Murashige and Skoog's medium supplemented with 2.22 µM benzyladenine (BA), 0.57 µM indole-3-acetic acid (IAA) and 41 µM adenine sulphate (AdS), the segments of basal halves of the first two leaves of a young seedling incubated on filter paper bridges in liquid medium took 20 – 25 d to differentiate shoot buds. The explants after transfer to solidified medium, with lower concentration of BA (0.22 μM) resulted in fast growing healthy shoots. The developed shoots (measuring ca. 3 cm) were rooted in a medium supplemented with 1.42 µM IAA. They were subsequently grown in pots with soil with more than 80 % transplantation success.

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Authors and Affiliations

  1. Tissue Culture Laboratory, National Botanical Research Institute, Lucknow, 226 001, India

    P. Misra

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  1. P. Misra
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Misra, P. Direct Differentiation of Shoot Buds from Leaf Explants of Cajanus Cajan L.. Biologia Plantarum 45, 347–351 (2002). https://doi.org/10.1023/A:1016205331021

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