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Occurrence of histone H1° protein in rat alveolar macrophages

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Abstract

Histone H1 of rat alveolar macrophages, neutrophilic granulocytes and monocytes extracted with 5% (v/v) perchloric acid was studied in order to see whether a protein similar to histone H1° of rat liver exists in these specialized cells. The biochemical methods used involved SDS and acid-urea polyacrylamide gel electrophoresis, gel filtration on BioGel P100 and raising antisera against chromatographically purified rat liver H1° and histone H1. The antiserum was applied for further characterization of the presumptive H1° fraction using ELISA and Western blot analysis.

The results from our studies showed that histone H1° protein is present in rat alveolar macrophages, monocytes and neutrophilic granulocytes, but its quantity in neutrophilic granulocytes is very much less than macrophages and monocytes.

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Parinaz Ghadam

  2. Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran

    Azra Rabbani

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  1. Parinaz Ghadam
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  2. Azra Rabbani
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Ghadam, P., Rabbani, A. Occurrence of histone H1° protein in rat alveolar macrophages. Mol Cell Biochem 236, 45–51 (2002). https://doi.org/10.1023/A:1016193909193

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