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Differential effects of des IGF-1 on ERKs, AKT-1 and P70 S6K activation in mouse skeletal and cardiac muscle

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Abstract

Alterations in the degree of the phosphorylation of ERK1/2, Akt-1 and p70 S6K in mouse skeletal and cardiac muscle was examined in vivo following an intraperitoneal injection of des IGF-I. Plasma levels of insulin, IGF-I and glucose were measured. The administration of des IGF-I had no effect on plasma levels of insulin, or IGF-I, but plasma glucose levels were decreased about 50% (p < 0.01). In both skeletal and cardiac muscle, des IGF-I increased the phosphorylation of Akt-1 at Ser 473 (p < 0.01) with no change in the phosphorylation of p44 and p42 MAP kinases at Thr202/Tyr204. The phosphorylation of p70 S6K at Thr421/Ser424 was increased in skeletal muscle (p < 0.01), but not in cardiac muscle. The phosphorylation of the nuclear transcription factor CREB phosphorylation at Ser 133 was not significantly changed in either skeletal or cardiac muscle. Des IGF-I increased the phosphorylation of the transcription factor FKHR in cardiac muscle only (p < 0.05). These data demonstrate that the administration of des IGF-I had differential effects on the activation of the MAP kinase and PI 3-kinase pathways in mouse skeletal and cardiac muscle.

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  1. Metabolic Biochemistry Laboratory, School of Kinesiology, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada

    M. Li, C. Li & W.S. Parkhouse

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  1. M. Li
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Li, M., Li, C. & Parkhouse, W. Differential effects of des IGF-1 on ERKs, AKT-1 and P70 S6K activation in mouse skeletal and cardiac muscle. Mol Cell Biochem 236, 115–122 (2002). https://doi.org/10.1023/A:1016164601887

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