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Root bacteria from nematicidal plants and their biocontrol potential against trichodorid nematodes in potato

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Abstract

Trichodorid nematodes (Nematoda: Trichodoridae) are vectors of tobacco rattle virus (TRV), one of the causal agents of spraing disease in potato. Root bacteria from nematicidal plants and their control potential against Trichodoridae were the focus of this study. Bacteria isolated from the roots of 12 nematicidal plants and potato were characterized for their production of hydrolytic enzymes, hydrogen cyanide, phenol oxidation ability and antifungal activity towards the potato pathogen Rhizoctonia solani. Based on these functional traits, bacteria isolates were selected and tested in greenhouse conditions on potato (cv. Saturna) for their effect on plant growth, and screened for nematicidal activity against Paratrichodorus pachydermus and Trichodorus primitivus in naturally infested soil. Sixteen bacteria isolates out of 44 reduced nematode densities by 50–100%. Nine selected isolated were further tested by bacterizing potato tubers (cv. King Edward) which were planted in a trichodorid and TRV-infested soil. Four bacterial isolates consistently reduced nematode densities (by 56.7–74.4%) with no visual negative effect on plant growth. These isolates were tentatively identified, partly by fatty acid methyl ester (FAME) analysis as: Stenotrophomonas maltophilia, Bacillus mycoides, Pseudomonas sp., and one unidentified bacterium. The isolates originated from potato, Plantago major, Thymus vulgaris and Asparagus officinalis, respectively. Two Pseudomonas isolates obtained from Zinnia elegans and selected for their strong nematicidal activity in soil screening tests, did not reduce the nematode population when tested on potato. It is concluded that plants releasing nematicidal compounds may harbour nematode-antagonistic bacteria as well.

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Authors and Affiliations

  1. Department of Ecology and Crop Production Science, Swedish University of Agricultural Sciences, Box 7043, SE-750 07, Uppsala, Sweden

    V. Insunza, S. Alström & K. B. Eriksson

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  1. V. Insunza
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  2. S. Alström
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  3. K. B. Eriksson
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Correspondence to V. Insunza.

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Insunza, V., Alström, S. & Eriksson, K.B. Root bacteria from nematicidal plants and their biocontrol potential against trichodorid nematodes in potato. Plant and Soil 241, 271–278 (2002). https://doi.org/10.1023/A:1016159902759

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