Abstract
The oxygen production of dark-adapted Photosystem II upon illumination by a series of single-turnover flashes shows a damped period four oscillation with flash number. The damping is attributed to `misses' resulting from a statistical probability that a reaction center fails to produce a stable charge separation after a saturating flash. The origin of misses is of interest because its probable dependence on flash number, in principle, affects the quantitative interpretation of all measurements on phenomena associated with the period four oscillation. We show that the kinetics of chlorophyll fluorescence yield transients induced by a flash series can be used to estimate the relative amplitudes of the miss probability on each flash. It is concluded that a major part of the misses must be caused by failure of the reduction of the oxidized primary electron donor chlorophyll P680+ by the secondary donor tyrosine YZ before the charge separation is lost by recombination. The probability of this failure is found to increase with the oxidation state of the oxygen-evolving complex: more than half of it occurs upon charge separation in the S3 state, which is attributed to the presence of YZ ox S2 in Boltzmann equilibrium with YZS3.
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References
Butler WL (1972) On the primary nature of fluorescence yield changes associated with photosynthesis. Proc Natl Acad Sci USA 69: 3420-3422
Christen G, Seeliger A and Renger G (1999) P680+ reduction kinetics and redox transition probability of the water oxidizing complex as a function of pH and H/D isotope exchange in spinach thylakoids. Biochemistry 38: 6082-6092
Dau H (1994) Molecular mechanisms and quantitative models of variable Photosystem II fluorescence. Photochem Photobiol 60: 1-23
Delosme R (1971) Photosynthèse.Variations du rendement de fluorescence de la chlorophylle in vivo sous l'action d'éclairs de forte intensité. C R Acad Sci Paris 272: 2828-2831
de Wijn R and van Gorkom HJ (2001) Kinetics of electron transfer from QA to QB in Photosystem II. Biochemistry 40: 11912-11922
de Wijn R, Schrama T and van Gorkom HJ (2001) Secondary stabilization reactions and proton-coupled electron transport in Photosystem II investigated by electroluminescence and fluorescence spectroscopy. Biochemistry 40: 5821-5834
Diner BA (1977) Dependence of the deactivation reactions of Photosysytem II on the redox state of the plastoquinone pool as varied under anaerobic conditions. Equilibria on the acceptor side of Photosystem II. Biochim Biophys Acta 460: 247-258
Duysens LNM and Sweers HE (1963) Mechanism of two photochemical reactions in algae as studied by means of fluorescence. In: Japanese Society of Plant Physiologists (ed) Microalgae and Photosynthetic Bacteria, pp 353-372. University of Tokyo Press, Tokyo
Duysens LNM, den Haan GA and van Best JA (1975) Rapid reactions of Photosystem II as studied by the kinetics of the fluorescence and luminescence of chlorophyll a in Chlorella pyrenoidosa. In Avron M (ed) Proceedings of the Third International Congress on Photosynthesis I, pp 1-12. Elsevier, Amsterdam
Geijer P, Morvaridi F and Styring S (2001) The S3 state of the oxygen-evolving complex in Photosystem II is converted to the S2Y· Z state at alkaline pH. Biochemistry 40: 10881-10891
Joliot P and Kok B (1975) Oxygen evolution in photosynthesis. In: Govindjee (ed) Bioenergetics of Photosynthesis, pp 387-412. Academic Press, New York
Lavorel J (1976) Matrix analysis of the oxygen evolving system of photosynthesis. J Theor Biol 57: 171-185
Paillotin G (1976) Movement of excitations in the photosynthetic domains of Photosystem II. J Theor Biol 58: 237-252
Prasil O, Adir N and Ohad I (1992) Dynamics of Photosystem II: mechanism of photoinhibition and recovery processes. In: Barber J (ed) The Photosystems: Structure, Function and Molecular Biology, pp 295-348. Elsevier, Amsterdam
Robinson HH and Yocum CF (1980) Cyclic photophosphorylation reactions catalyzed by ferredoxin, methyl viologen and anthraquinone sulfonate. Use of photochemical reactions to optimize redox poising. Biochim Biophys Acta 590: 97-106
Thibault P (1978) A new attempt to study the oxygen evolving system of photosynthesis: determination of transition probabilities of a state i. J Theor Biol 73: 271-284
van Gorkom HJ (1986) Fluorescence measurements in the study of Photosystem II electron transport. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp 267-289. Academic Press, Orlando, Florida
van Gorkom and Schelvis (1993) Kok's oxygen clock: what makes it tick? The structure of P680 and consequences of its oxidizing power. Photosynth Res 38: 297-301
van Gorkom HJ, Thielen APGM and Gorren ACF (1982) The secondary electron acceptor of Photosystem II. In: Trumpower BL (ed) The Function of Quinones in Energy Conserving Systems, pp 213-225. Academic Press, New York/London
Vernotte C, Etienne AL and Briantais JM (1979) Quenching of the system II chlorophyll fluorescence by the plastoquinone pool. Biochim Biophys Acta 545: 519-527
Vos MH, van Gorkom HJ and van Leeuwen PJ (1991) An electroluminescence study of stabilization reactions in the oxygenevolving complex of Photosystem II. Biochim Biophys Acta 1056: 27-39
Zankel KL (1973) Rapid fluorescence changes observed in chloroplasts: their relationship to the O2 evolving system. Biochim Biophys Acta 325: 138-148
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de Wijn, R., van Gorkom, H.J. S-state dependence of the miss probability in Photosystem II. Photosynthesis Research 72, 217–222 (2002). https://doi.org/10.1023/A:1016128632704
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DOI: https://doi.org/10.1023/A:1016128632704