Abstract
The lipid layer membranes were fabricated on the glassy carbon electrode (GC) and demonstrated to be bilayer lipid membranes by impedance spectroscopy. The formation of incorporated poly L-glutamate bilayer lipid membrane was achieved. The ion channel behavior of the incorporated poly L-glutamate membrane was determined. When the stimulus calcium cations were added into the electrolyte, the ion channel was opened immediately and exhibited distinct channel current. Otherwise, the ion channel was closed. The cyclic voltammogram at the GC electrode coated with incorporated poly L-glutamate DMPC film response to calcium ion is very fast compared with that at the GC electrode coated only with DMPC film. Ion channel current is not dependent on the time but on the concentration of calcium. The mechanism of the ion channel formation was investigated.
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REFERENCES
Dalian Light Industry Institute (1994). Biochemistry, Dalian Light Industry Publishing Company, China.
Fettiplace, R., Andrews, D. M., and Haydon, D. A. (1971). J. Membr. Biol. 5, 277–296.
Hall, E. A. H., Skinner, N. G., Jung, C., and Szunerits, S. (1995). Electroanalysis 7, 830–837.
Horber, J. H., Lang, C. A., Hansch, T. W., Heckl, W. M., and Mohwald, H. (1988). Chem. Phys. Lett. 145, 151–158.
Jiang, D., Diao, P., Tong, R., Gu, D., and Zhong, B. (1998), Bioelectrochem. Bioenerg. 44, 285–288.
Maeda, M., Tsuzaki, Y., Nakano, K., and Takagi, M. (1990). J. Chem. Soc., Chem. Commun. 1529–1531.
Martymski, T., and Tien, H. T. (1991). Bioelectrochem. Bioenerg. 25, 317–324.
Nikolelis, D. P., and Krull, U. J. (1993). Electroanalysis 5, 539–545.
Nikolelis, D. P., and Krull, U. J. (1994). Anal. Chim. Acta 288, 187–192.
Nikolelis, D. P., and Siontorou, C. G. (1995). Anal. Chem. 67, 936–944.
Patolsky, F., Zayats, M., Katz, E., and Willner, I. (1999). Anal. Chem. 71, 3171–3180.
Plant, A. L. (1999). Langmuir 15, 5128–5135.
Ren, X., and Pickup, P. G. (1997). J. Electroanal. Chem. 420, 251–257.
Sugawara, M., Kojima, K., Sazawa, H., and Umezawa, Y. (1987). Anal. Chem. 59, 2842–2846.
Tien, H. T. (1974). Bilayer Lipid Membranes (BLM): Theory and Practice, Marcel Dekker, New York.
Tien, H. T. (1988). J. Clin. Lab. Anal. 2, 256.
Tien, H. T. (1989). Prog. Surf. Sci. 30, 1–199.
Tien, H. T., and Ottova, A. (1998). Electrochim. Acta 43, 3587–3610.
Tien, H. T., and Salamon, Z. (1989). Bioelectrochem. Bioenerg. 22, 211–218.
Watt, A., Harlos, K., and Marsh, D. (1981). Biochim. Biophys. Acta 645, 91–96.
Wu, Z., Tang, J., Cheng, Z., Yang, X., and Wang, E. (2000). Anal. Chem. 72, 6030–6033.
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Tong, Y., Wu, Z., Han, X. et al. Electrochemical Study of Ion Channel Behavior in Incorporated Poly L-glutamate Bilayer Lipid Membranes. J Bioenerg Biomembr 34, 185–191 (2002). https://doi.org/10.1023/A:1016031403141
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DOI: https://doi.org/10.1023/A:1016031403141