Abstract
Identification of interaction partners opens a way to direct functional characterization of proteins. Several cDNAs coding for potential partners of protein kinase MAK-V/Hunk were isolated using two-hybrid cloning in yeast. Based on the partner properties, MAK-V/Hunk was assumed to play a role in tumorigenesis and tumor progression. With the previous results of two-hybrid cloning, MAK-V/Hunk was shown to participate in vesicular transport.
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Korobko, I.V., Korobko, E.V., Chupikova, N.I. et al. Functional Characterization of Protein Kinase MAC-V with the Use of Two-Hybrid Cloning in Yeast. Molecular Biology 36, 385–388 (2002). https://doi.org/10.1023/A:1016011430092
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DOI: https://doi.org/10.1023/A:1016011430092