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The Measurement of Warfarin Enantiomers in Serum Using Coupled Achiral/Chiral, High-Performance Liquid Chromatography (HPLC)

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Abstract

An assay for the serum concentration of the enantiomers of warfarin, R-warfarin and S-warfarin, has been developed using a bovine serum albumin chiral stationary phase (BSA-CSP) coupled to a Pinkerton internal-surface reverse-phase (ISRP) achiral column. The ISRP column is used to separate R,S-warfarin from the serum components and warfarin metabolites and to quantitate the total R,S-warfarin concentration. The eluent containing R,S-warfarin is then selectively transferred to the BSA-CSP, where the enantiomers are stereochemically resolved (α = 1.19) and the enantiomeric composition is determined. This system is sensitive and accurate, does not require extensive precolumn manipulations, and can be automated for use in large-scale clinical studies.

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Authors and Affiliations

  1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China

    Ya-Qin Chu

  2. Pharmaceutical Division, Section of Pharmacokinetics and Pharmacodynamics, St. Jude Children's Research Hospital, Memphis, Tennessee

    Irving W. Wainer

Authors
  1. Ya-Qin Chu
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  2. Irving W. Wainer
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Chu, YQ., Wainer, I.W. The Measurement of Warfarin Enantiomers in Serum Using Coupled Achiral/Chiral, High-Performance Liquid Chromatography (HPLC). Pharm Res 5, 680–683 (1988). https://doi.org/10.1023/A:1015991324316

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  • DOI: https://doi.org/10.1023/A:1015991324316

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