Skip to main content
SpringerLink
Log in

Analysis of Hepatic Transport of Cefpiramide in Rats with Obstructive Jaundice by Using Isolated Hepatocytes

  • Published:
Pharmaceutical Research Aims and scope Submit manuscript

Abstract

The mechanism of the diminished biliary clearance of cefpiramide (CPM) in rats with obstructive jaundice (OJ) was investigated by using isolated hepatocytes. The kinetics of CPM uptake by hepatocytes isolated from normal rats and rats with OJ could be explained by the combination of saturable carrier-mediated and nonsaturable first-order rate processes. The maximum uptake rate (V max) of the carrier-mediated process was significantly decreased in OJ, compared with normal hepatocytes, while the Michaelis constant (K m) and the first-order rate constant (k d) were not significantly different. This result indicated that the number of CPM transport carriers was decreased in OJ hepatocytes. Further, no CPM uptake occurred from the serum of OJ rats into normal hepatocytes. Partial recovery of CPM uptake after treatment of OJ serum with activated charcoal suggested the accumulation of inhibitors of CPM uptake in OJ serum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. T. Kimura, T. Yamao, S. Nakayama, S. Honge, T. Kawakami, Y. Kurosaki, and T. Nakayama. J. Pharmacobio-Dyn. 11:839–848 (1988).

    Google Scholar 

  2. A. Tsuji, T. Terasaki, I. Tamai, E. Nakashima, and K. Takanosu. J. Pharm. Pharmacol. 37:55–57 (1985).

    Google Scholar 

  3. L. R. Schwarz, R. Burr, M. Schwenk, E. Pfaff, and H. Greim. Eur. J. Biochem. 55:617–623 (1975).

    Google Scholar 

  4. M. Schwenk, R. Burr, L. Schwarz, and E. Pfaff. Eur J. Biochem. 64:189–197 (1976).

    Google Scholar 

  5. A. Blom, K. Keulemans, and Dirk K. F. Meijer. Biochem. Pharmacol. 30:1809–1816 (1981).

    Google Scholar 

  6. P. Moldeus, J. Hogberg, and S. Orrenius. Methods Enzymol. 52:60–71 (1978).

    Google Scholar 

  7. R. F. Chen. J. Biol. Chem. 242:173–181 (1967).

    Google Scholar 

  8. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall. J. Biol. Chem. 193:265–275 (1951).

    Google Scholar 

  9. K. Yamaoka, Y. Tanigawara, T. Nakagawa, and T. Uno. J. Pharmacobio-Dyn. 4:879–885 (1981).

    Google Scholar 

  10. A. Tsuji, T. Terasaki, K. Takanosu, I. Tamai, and E. Nakashima. Biochem. Pharmacol. 35:151–158 (1986).

    Google Scholar 

  11. J. Reichen, C. Hoilien, M. Le, and R. H. Jones. Hepatology 7:67–70 (1987).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Okayama, 700, Japan

    Tadanao Yamao, Seiya Nakayama, Yuji Kurosaki, Taiji Nakayama & Toshikiro Kimura

Authors
  1. Tadanao Yamao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seiya Nakayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuji Kurosaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Taiji Nakayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Toshikiro Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamao, T., Nakayama, S., Kurosaki, Y. et al. Analysis of Hepatic Transport of Cefpiramide in Rats with Obstructive Jaundice by Using Isolated Hepatocytes. Pharm Res 7, 1038–1041 (1990). https://doi.org/10.1023/A:1015991116912

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015991116912

Search

Navigation