Abstract
A liquid chromatographic method for the determination of ACC-9358, an antiarrhythmic agent, in human plasma is described. ACC-9358 was extracted from plasma into chloroform and back extracted into 0.01 N hydrochloric acid. ACC-9358 in the acidic extract was eluted from a Novapak C18 column using a 87:13:0.1 (by volume) mixture of phosphate buffer (pH 3.0), acetonitrile, and triethylamine as the mobile phase and measured at 280 nm. The average extraction recoveries were 78.4 and 85.4% at the 0.5- and 5-µg/ml level, respectively. Standard curves were linear and reproducible over the concentration range of 0.01–10 µg/ml, with coefficients of determination better than 0.999. Coefficients of variation for both within-day and day-to-day analysis were less than 11%. The assay method is sensitive, reproducible, and suitable for disposition studies of ACC-9358 in humans.
Similar content being viewed by others
REFERENCES
R. D. Reynolds, B. S. Brown, and C. Y. Sum. Pharmacologist 26:211 (1984) (abstr.).
C. Y. Quon, L. Bavda, H. Stampfli, and Y. C. Chan. Pharmacologist 27:270 (1985) (abstr.).
Y. C. Chan, L. Bavda, and C. Y. Quon. Fed. Proc. 45:337 (1986) (abstr.).
SAS Institute. SAS Users Guide: Statistics, 1982 ed., SAS Institute, Cary, N.C., 1982.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Achari, R., Thomas, D.A. Determination of a Novel Antiarrhythmic Agent ACC-9358 in Human Plasma by High-Performance Liquid Chromatography. Pharm Res 5, 190–192 (1988). https://doi.org/10.1023/A:1015973108928
Issue Date:
DOI: https://doi.org/10.1023/A:1015973108928