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Pharmaceutical Research

, Volume 7, Issue 10, pp 1042–1047 | Cite as

Use of Shed Snake Skin as a Model Membrane for in Vitro Percutaneous Penetration Studies: Comparison with Human Skin

  • Tomoo Itoh
  • Jun Xia
  • Ravi Magavi
  • Toshiaki Nishihata
  • J. Howard Rytting
Article

Abstract

The potential usefulness of shed snake skin as a model membrane for transdermal research was examined. There are similarities between shed snake skin and human stratum corneum in terms of structure, composition, lipid content, water permeability, etc. The permeability of various compounds and the contribution of several functional groups to the permeability were also found to be similar between shed snake skin and human skin. Moreover, the permeability of compounds through shed snake skin was increased by Azone, one of the most extensively studied transdermal penetration enhancers. Considering the similarities between shed snake skin and human skin, ease of storage and handling, and low cost, shed snake skin may offer a good model membrane for transdermal research.

skin penetration transdermal shed snake skin functional group contribution Azone Elaphe obsoleta 

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REFERENCES

  1. 1.
    R. J. Scheuplein and I. H. Blank. Physiol. Rev. 51 (4):702–747Google Scholar
  2. 2.
    M. J. Bartek, J. A. LaBudde, and H. I. Maibach. J. Invest. Dermatol. 58 (3):114–123 (1972).Google Scholar
  3. 3.
    P. Campbell, T. Watanabe, and S. K. Chandrasekaran. Fed. Proc. 35:639 (1976).Google Scholar
  4. 4.
    M. Walker, P. H. Dugard, and R. C. Scott. Hum. Toxicol. 2:561–562 (1983).Google Scholar
  5. 5.
    R. C. Wester and P. K. Noonan. Int. J. Pharm. 7:99–110 (1980).Google Scholar
  6. 6.
    G. L. Flynn, H. Durrheim, and W. I. Higuchi. J. Pharm. Sci. 70 (1):52–56 (1981).Google Scholar
  7. 7.
    D. S-L. Chow, I. Kaka, and T. I. Wang. J. Pharm. Sci. 73 (12):1794–1799 (1984).Google Scholar
  8. 8.
    J. Hadgraft and G. Ridout. Int. J. Pharm. 39:149–156 (1987).Google Scholar
  9. 9.
    J. Hadgraft and G. Ridout. Int. J. Pharm. 42:97–104 (1988).Google Scholar
  10. 10.
    P. F. A. Maderson. Am. Zool. 12:159–171 (1972).Google Scholar
  11. 11.
    A. M. Kligman. Am. Heart J. 108 (1):200–206 (1984).Google Scholar
  12. 12.
    D. Dupuis, A. Rougier, R. Roguet, C. Lotte, and G. Kalopissis. J. Invest. Dermatol. 82:353–356 (1984).Google Scholar
  13. 13.
    T. K. Banerjee and A. K. Mittal. In R. I. C. Spearman and P. A. Riley (eds.), The Skin of Vertebrates, Academic Press, London, 1978, pp. 23–34.Google Scholar
  14. 14.
    L. Landmann. J. Morphol. 162:93–126 (1979).Google Scholar
  15. 15.
    J. B. Roberts and H. B. Lillywhite. Science 207:1077–1079 (1980).Google Scholar
  16. 16.
    L. Landmann, C. Stolinski, and B. Martin. Cell Tissue Res. 215:369–382 (1981).Google Scholar
  17. 17.
    P. Licht and A. F. Bennett. Copeia 702–707 (1972).Google Scholar
  18. 18.
    A. F. Bennett and P. Licht. Comp. Biochem. Physiol. 52A:213–215 (1975).Google Scholar
  19. 19.
    J. B. Roberts and H. B. Lillywhite. J. Exp. Zool. 228:1–9 (1983).Google Scholar
  20. 20.
    G. S. Berenson and G. E. Burch. Am. J. Trop. Med. 31:842–853 (1951).Google Scholar
  21. 21.
    M. A. Lampe, M. L. Williams, and P. M. Elias. J. Lipid Res. 24:131–140 (1983).Google Scholar
  22. 22.
    M. A. Lampe, A. C. Burlingame, J. Whitney, M. L. Williams, B. E. Brown, E. Roitman, and P. M. Elias. J. Lipid Res. 24:120–130 (1983).Google Scholar
  23. 23.
    N. Nicolaides. Science 186:19–26 (1974).Google Scholar
  24. 24.
    J. B. Roberts and G. M. Helmkamp. Mid-west Regional Meeting of Society for Investigative Dermatology, Chicago, 1982.Google Scholar
  25. 25.
    D. G. Ahern and D. T. Downing. Lipids 9 (1):8–14 (1974).Google Scholar
  26. 26.
    R. Ibuki. Ph.D. thesis, Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, 1985.Google Scholar
  27. 27.
    M. Akazawa, T. Itoh, K. Masaki, B. T. Nghiem, N. Tsuzuki, R. Konishi, and T. Higuchi. Int. J. Pharm. 50:53–60 (1989).Google Scholar
  28. 28.
    K. Sugibayashi, K. Hosoya, Y. Morimoto, and W. I. Higuchi. J. Pharm. Pharmacol. 37:578–580 (1985).Google Scholar
  29. 29.
    Y. Morimoto, K. Sugibayashi, K. Hosoya, and W. I. Higuchi. Int. J. Pharm. 32:31–38 (1986).Google Scholar
  30. 30.
    R. B. Stoughton and W. O. McClure. Drug. Dev. Ind. Pharm. 9 (4):725–744 (1983).Google Scholar
  31. 31.
    P. Agrawala and W. A. Ritschel. J. Pharm. Sci. 77 (9):776–778 (1988).Google Scholar
  32. 32.
    P. M. Elias, E. R. Cooper, A. Korc, and B. E. Brown. J. Invest. Dermatol. 76:297–301 (1981).Google Scholar
  33. 33.
    I. H. Blank. J. Invest. Dermatol. 18:433–440 (1952).Google Scholar
  34. 34.
    M. S. Roberts, R. A. Anderson, and J. Swarbrick. J. Pharm. Pharmacol. 29:677–683 (1977).Google Scholar
  35. 35.
    R. J. Scheuplein, I. H. Blank, G. J. Brauner, and D. J. MacFarlane. J. Invest. Dermatol. 52 (1):63–70 (1969).Google Scholar
  36. 36.
    B. D. Anderson, W. I. Higuchi, and P. V. Raykar. Pharm. Res. 5 (9):566–573 (1988).Google Scholar
  37. 37.
    O. Wong, J. Huntington, T. Nishihata, and J. H. Rytting. Pharm. Res. 6 (4):286–295 (1989).Google Scholar
  38. 38.
    R. C. Wester and H. I. Maibach. In Maibach and Lowe (eds.), Models in Dermatology, Karger, Basel, 1985, pp. 159–169.Google Scholar
  39. 39.
    A. Rougier, C. Lotte, and H. I. Maibach. J. Pharm. Sci. 76 (6):451–454 (1987).Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Tomoo Itoh
    • 1
  • Jun Xia
    • 1
  • Ravi Magavi
    • 1
  • Toshiaki Nishihata
    • 1
    • 2
  • J. Howard Rytting
    • 1
  1. 1.Department of Pharmaceutical ChemistryUniversity of KansasLawrence
  2. 2.Upjohn Tsukuba Research LaboratoriesIbaraki-kenJapan

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