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Measurement of Underivatized Propranolol Enantiomers in Serum Using a Cellulose –Tris(3,5 -Dimethylphenylcarbamate) High-Performance Liquid Chromatographic (HPLC) Chiral Stationary Phase

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Abstract

A commercially available high-performance liquid chromatographic (HPLC) chiral stationary phase (HPLC-CSP) has been used to measure serum levels of d- and l-propranolol. The HPLC-CSP is based upon cellulose–tris(3,5-dimethylcarbamate) and is able to stereochemically resolve d- and l-propranolol without precolumn derivatization using a mobile phase composed of hexane:2-propranol:N,N-dimethyloctylamine (92:8:0.01, v/v/v). Under these conditions the observed stereochemical resolution (α) of the two enantiomers was α = 2.2. A subject's concentration–time curve of the two isomers was determined following the ingestion of 160 mg racemic propranolol.

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Author notes

  1. Robert J. Straka

    Present address: Dept. of Pharmacy Practice, College of Pharmacy, University of Minnesota, Rm 7115, HSCUF, 308 Harvard St, S.E., Minneapolis, Minnesota, 55455

Authors and Affiliations

  1. Department of Clinical Pharmacy, University of Tennessee, Memphis, Memphis, Tennessee, 38163

    Robert J. Straka, Richard L. Lalonde & Irving W. Wainer

  2. Pharmaceutical Division, Section of Pharmacokinetics and Pharmacodynamics, St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, Tennessee, 38105

    Irving W. Wainer

Authors
  1. Robert J. Straka
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  2. Richard L. Lalonde
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  3. Irving W. Wainer
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Straka, R.J., Lalonde, R.L. & Wainer, I.W. Measurement of Underivatized Propranolol Enantiomers in Serum Using a Cellulose –Tris(3,5 -Dimethylphenylcarbamate) High-Performance Liquid Chromatographic (HPLC) Chiral Stationary Phase. Pharm Res 5, 187–189 (1988). https://doi.org/10.1023/A:1015921124857

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