Abstract
The possible mechanism of hepatic uptake of colchicine (CLC), a microtubule system disrupting agent, was examined using isolated rat hepatocytes. The existence of a carrier-mediated active transport system for CLC was demonstrated. This transport system is saturable, is affected by metabolic inhibitors (dinitrophenol, KCN) and a SH-group blocker (p-hydroxymercuribenzoic acid but not N-ethylmaleimide), and is sensitive to temperature. Ouabain, an inhibitor of Na+, K+-ATPase, does not affect the transport system of CLC.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
L. Wilson and K. W. Farrell. Ann. N.Y. Acad. Sci. 416:690–708 (1986).
J. Watanabe, S. Kanamura, K. Kanai, M. Asada-Kubota, and M. Oka. J. Endocrinol. 106:125–131 (1985).
C. F. Whitfield and M. E. Schworer. Arch. Biochem. Biophys. 191:727–733 (1987).
S. G. Barnwell and R. Coleman. Biochem. J. 216:409–414 (1983).
S. G. Barnwell, P. J. Lowe, and R. Coleman. Biochem. J. 220:723–731 (1984).
R. S. Bajwa and J. M. Fujimoto. Biochem. Pharmacol. 32:85–90 (1983).
S. S. Kaufman, D. J. Tuma, M. F. Sorrellm, and J. A. Vanderhoof. Am. J. Physiol. 247:G311–G318 (1984).
V. Ling and L. H. Thompson. Cell Physiol. 83:103–116 (1979).
S. A. Carlsen, J. E. Till, and V. Ling. Biochim. Biophys. Acta 455:900–912 (1976).
V. Ling, N. Kartuer, T. Sudo, L. Siminovitch, and J. R. Riordan. Cancer Treat. Rep. 67:869–874 (1983).
Y. P. See, S. A. Carlsen, J. E. Till, and V. Ling. Biochim. Biophys. Acta 373:242–252 (1974).
M. N. Berry and D. S. Friend. J. Cell Biol. 43:506–520 (1969).
H. Baur, S. Kasperek, and E. Pfaff. Hopp-Seyler Z. Physiol. Chem. 356:827–838 (1975).
K. Yamaoka, Y. Tanigawara, T. Nakagawa, and T. Uno. J. Pharmacobio. Dyn. 4:879–885 (1981).
D. L. Eaton and C. D. Klaassen. J. Pharmacol. Exp. Ther. 205:480–488 (1978).
D. G. Vogel, R. L. Margolis, and N. K. Mottet. Toxicol. Appl. Pharmacol. 80:473–486 (1985).
E. Baraona, F. Finkelman, and N. K. Matsuda. Anal. Biochem. 130:302–310 (1983).
S. B. Mizel and L. Wilson. Biochemistry 11:2573–2578 (1974).
R. D. Irons, R. W. Pfeifer, T. M. Aune, and C. W. Pierce. J. Immunol. 133:2032–2036 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wierzba, K., Sugiyama, Y., Okudaira, K. et al. Uptake of Colchicine, a Microtubular System Disrupting Agent, by Isolated Rat Hepatocytes. Pharm Res 6, 235–238 (1989). https://doi.org/10.1023/A:1015917601349
Issue Date:
DOI: https://doi.org/10.1023/A:1015917601349