Abstract
A series of carboxyfluorescein (CF)-containing multilamellar vesicle (MLV) dispersions was prepared and extruded through polycarbonate membranes ranging in size from 0.2 to 5 µm. Vesicle dispersions were nebulized for 80 min using a Collison nebulizer, and the release of CF was monitored during nebulization. Solute retention was dependent upon the size of the vesicles and leakage ranged from 7.9 ± 0.4% (N = 3) for vesicles extruded through 0.2-µm filters to 76.8 ± 5.9% (N = 3) for liposomes that were not filtered. Solute release profiles obtained over ≥420-min nebulization periods conformed to a two-compartment kinetic model and exhibited a “fast” initial phase (k l = 0.052 ± 0.0043) followed by a “slow” terminal phase (k 2 = 0.0034 ± 0.00018). The results show that CF retention can be increased by nebulizing small vesicles and modeling suggests that the rate of CF leakage from the bilayers is faster than from the core of the liposomes.
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REFERENCES
R. W. Niven and H. Schreier. Nebulization of liposomes. I. Effects of lipid composition. Pharm. Res. 7:1127–1133 (1990).
B. E. Gilbert, H. R. Six, S. Z. Wilson, P. R. Wyde, and V. Knight. Small particle aerosols of enviroxime-containing liposomes. Antivir. Res. 9:355–365 (1988).
T. T. Mercer, M. I. Tillery, and H. Y. Chow. Operating characteristics of some compressed air nebulizers. Am. Ind. Hyg. Assoc. J. 29:66–78 (1968).
H. W. Young, J. W. Dominik, J. S. Walker, and E. W. Larson. Continuous aerosol therapy system using a modified Collison nebulizer. J. Clin. Microbiol. 5:131–136 (1977).
C. O. Callaghan, A. R. Clarke, and A. D. Milner. Inaccurate calculation of drug output from nebulisers. Eur. J. Pediat. 148:473–474 (1989).
J. A. Wood, R. S. E. Wilson, and C. Bray. Changes in salbutamol concentration in the reservoir solution of a jet nebulizer. Br. J. Dis. Chest 80:164–169 (1986).
K. R. May. The Collison nebulizer: Description, performance and application. J. Aerosol Sci. 4:235–243 (1973).
L. D. Mayer, M. B. Bally, M. J. Hope, and P. R. Cullis. Techniques for encapsulating bioactive agents into liposomes. Chem. Phys. Lipids. 40:333–345 (1986).
S. P. Newman, P. G. D. Pellow, M. M. Clay, and S.W. Clarke. Evaluation of jet nebulizers for use with gentamicin solution. Thorax 40:671–676 (1985).
R. V. Lourenço and E. Cotromanes. Clinical aerosols. I. Characterization of aerosols and their diagnostic uses. Arch. Intern. Med. 142:2163–2172 (1982).
K. M. G. Taylor, G. Taylor, I. W. Kellaway, and J. Stevens. The stability of liposomes to nebulization. Int. J. Pharm. 58:57–61 (1990).
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Niven, R.W., Speer, M. & Schreier, H. Nebulization of Liposomes. II. The Effects of Size and Modeling of Solute Release Profiles. Pharm Res 8, 217–221 (1991). https://doi.org/10.1023/A:1015896121377
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DOI: https://doi.org/10.1023/A:1015896121377