Skip to main content
SpringerLink
Log in

Absorption and Disposition of Colloidal Drug Delivery Systems. I. High-Performance Liquid Chromatographic (HPLC) Analysis of a Cyclosporin Emulsion

  • Published:
Pharmaceutical Research Aims and scope Submit manuscript

Abstract

The amount of cyclosporin A in an oil-in-water emulsion drug delivery system was determined by HPLC. The direct extraction and analysis of an intact emulsion were compared to the analysis of a cracked emulsion and an olive oil solution of the drug. The intra- and interday variability for the intact emulsion was less than 10% from 35 to 150 µg/ml, with recovery of 94%. Comparison of the assay results obtained with the emulsion and the olive oil solution gave a highly correlated regression line with a small intercept and a slope close to unity. Thus, the direct extraction and HPLC analysis of drugs in emulsions may be a viable approach to evaluate drug content

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. M. Y. Levy and S. Benita. Int. J. Pharm. 54:103–112 (1989).

    Google Scholar 

  2. R. Kirsh, R. Goldstein, J. Tarloff, D. Parris, J. Hook, N. Hanna, P. Bugelski, and G. Poste. J. Infect. Dis. 158:1065–1070 (1988).

    Google Scholar 

  3. C. L. Fortner, W. R. Grove, D. Bowie, and M. D. Walker. Am. J. Hosp. Pharm. 32:582–584 (1975).

    Google Scholar 

  4. S. S. Davis. Pharm. Tech. 6:110–117 (1987).

    Google Scholar 

  5. B. D. Tarr and S. H. Yalkowsky. Pharm. Res. 6:40–43 (1989).

    Google Scholar 

  6. R. Jeppsson and S. Ljungberg. Acta Pharmacol. Toxicol. 36:312–320 (1975).

    Google Scholar 

  7. Y. Mizushima, T. Hamano, and K. Yokoyama. J. Pharm. Pharmacol. 34:49–50 (1982).

    Google Scholar 

  8. H. Sasaki, T. Kakutani, M. Hashida, and H. Sezaki. J. Pharm. Pharmacol. 37:461–465 (1985).

    Google Scholar 

  9. R. Christinat and H. W. Zulliger. Drug Res. 341:551–553 (1984).

    Google Scholar 

  10. D. L. Teagarden, B. D. Anderson, and W. J. Petre. Pharm. Res. 6:210–215 (1989).

    Google Scholar 

  11. R. J. Sawchuk and L. L. Carter. Clin. Chem. 27:1368–1371 (1981).

    Google Scholar 

  12. S. Bolton. Pharmaceutical Statistics, Marcel Dekker, New York, 1984, pp. 190–192.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Clinical Pharmacokinetics Laboratory, Department of Pharmaceutics, School of Pharmacy, University of Maryland, 20 North Pine Street, Baltimore, Maryland, 21201

    Susan J. Corvari, R. Gary Hollenbeck, James Leslie, Karen I. Plaisance & David Young

Authors
  1. Susan J. Corvari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Gary Hollenbeck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James Leslie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Karen I. Plaisance
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. David Young
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Corvari, S.J., Hollenbeck, R.G., Leslie, J. et al. Absorption and Disposition of Colloidal Drug Delivery Systems. I. High-Performance Liquid Chromatographic (HPLC) Analysis of a Cyclosporin Emulsion. Pharm Res 8, 40–42 (1991). https://doi.org/10.1023/A:1015870020952

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015870020952

Search

Navigation