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Method for the Determination of Diphenhydramine in Rabbit Whole Blood by High-Performance Liquid Chromatography (HPLC) with Ultraviolet (UV) Detection in Conjunction with Gas Chromatography (GC) with Mass Selective Detection (MSD)

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Abstract

An HPLC/GC-MSD method for the determination of diphenhydramine in rabbit whole blood has been developed and validated. This method is based on a liquid–liquid extraction and reversed-phase Chromatography with ultraviolet absorbance detection monitored at 258 nm. HPLC eluant fractions containing diphenhydramine and the internal standard, orphenadrine, were collected, reex-tracted, then subjected to GC-MSD analysis. Whole blood was utilized, thereby decreasing the required sample volume and increasing the sensitivity of the assay. Diphenhydramine concentrations can be quantitated over a range of 1 to 1000 ng/ml whole blood.

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Authors and Affiliations

  1. Pharmacokinetics Laboratory, University of Missouri—Kansas City, School of Pharmacy, Kansas City, Missouri, 64108

    Karen M. Walters-Thompson & William D. Mason

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  1. Karen M. Walters-Thompson
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  2. William D. Mason
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Walters-Thompson, K.M., Mason, W.D. Method for the Determination of Diphenhydramine in Rabbit Whole Blood by High-Performance Liquid Chromatography (HPLC) with Ultraviolet (UV) Detection in Conjunction with Gas Chromatography (GC) with Mass Selective Detection (MSD). Pharm Res 9, 929–932 (1992). https://doi.org/10.1023/A:1015861218342

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