Abstract
In this study a simple electrophoresis approach has been proposed for assessing DNA damage per chromosome in vitro. Novel procedures of gel casting, sample loading, electrophoresis and quantification of damage have been suggested. Sets of Saccharomyces cerevisiae chromosomes subjected to DNA damage by Bleomycin, Co60-γ-radiation alone and in combination with Hoechst were studied in detail. Statistical analyses showed that damage induced by Bleomycin bore linear positive correlation with %GA (r=0.97) and %GT (r=0.61) contents of chromosomes. Samples pre-treated with Hoechst showed much less damage by Co60-γ-irradiation as compared to samples not treated with Hoechst but exposed to Co60-γ-irradiation. The `protective effect of Hoechst' bore linear positive correlation (r=0.8) with %TAT content of chromosomes.
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References
Gebhart E, Neubauer S, Schmitt G, Birkenhake S & Dunst J (1996) Radiat. Res. 145: 47–52
Rabbitts TH (1994) Nature 372: 143–149
Russell NS, Arlett CF, Bartelink H & Beff AC (1995) Int. J. Radiat. Biol. 68: 185–196
Bala M & Jain V (1994) Indian J. Exp. Biol. 32: 860–864
Bala M & Jain V (1997) Indian J. Biochem. Biophys. 34: 438–493
Bala M, Sharma AK & Goel HC (2001) J. Radiat. Res. 42: 285–294
Bala M & Jain V (1996) Ind. J. Exptl. Biol. 34: 18–26
Bala M (2000) Presented at XXI annual Conference, Indian Association of Biomedical Scientists, Oct 13-15, Rajah Muthiah Medical College, Annamalai University, Annamalai Nagar-608 002, India
Lobrich M, Ikpeme S, Haub P, Weber KJ & Kiefer J (1993) Int. J. Radiat. Biol. 64: 539–546
Friedl AA, Kraxenbergen A & Eckardt-Schupp F (1995) Methods: A companion to methods in enzymology 7: 205–218
Sax K (1940) Genetics. 25: 41–68
Xiao Y & Natarajan AT (1999) Int. J. Radiat. Biol. 75: 419–427
Pjura PE, Grzeskowiak K & Dickerson RE (1987) J. Mol. Biol. 197: 257–271
Martin RF & Denison L Int. J. Radiat. Oncol. Biol. Phys. (1992) 23: 579–584
Gabelica V, De Pauw E & Rosu FJ. (1999) Mass Spectrometry 34: 1328–1337
Utsuno K, Maeda Y & Tsuboi M (1999) Chem. Pharmaceut. Bull. 47: 1363–1368
Tang XL & Wang AH (1999) Pharmacol. Therapeut. 83: 181–215
Giloni L, Takeshita M, Johnson F, Iden C & Grollman AP (1981) J. Biol. Chem. 256: 8608–8615
Takeshita M, Grollman AP, Ohtsubo E & Ohtsubo H (1978) Proceedings of the National Academy of Sciences, USA 75: 5983–5987
Goffeau A, Aert R, Agostini-Carbone ML et al. (1997) Nature 387: 5
Cloos J, Nieuwenhuis EJ, Boomsma DI et al. (1999) J. Natl Can. Inst. 91: 1125–1130
Lango E & Vezinhet F (1993) Appl. Environ. Microbiol. 59: 322–326
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Bala, M., Mathew, L. An in vitro approach to study chromosomal DNA damage. Mol Biol Rep 28, 199–207 (2001). https://doi.org/10.1023/A:1015774006757
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DOI: https://doi.org/10.1023/A:1015774006757