Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA

Abstract

Diversity in 28 accessions representing 12 species of the genus, Cajanus arranged in 6 sections including 5 accessions of the cultivated species, C. cajan, and 4 species of the genus Rhyncosia available in the germplasm collection at ICRISAT was assessed using RFLP with maize mtDNA probes. Cluster analysis of the Southern blot hybridization data with 3 restriction enzymes – 3 probe combinations placed the genus Rhyncosia in a major group well separated from all the species belonging to the genus Cajanus. Within the genus Cajanus, the 4 accessions of C. platycarpus belonging to section Rhynchosoides formed a separate group in contrast to those in other sections of pigeonpea. In the section, Cajanus all the 5 accessions of C. cajan were grouped together and C. cajanifolius belonging to the same section was in a subgroup by itself closer to the main group. The four accessions of C. scarabaeoides, were together and the other species belonging to section Cantharospermum were in different subgroups. The intra-specific variation was seen even within accessions of certain pigeonpea wild species such as C. scarabaeoides, C. platycarpus, C. acutifolius, and even the cultivated species of C. cajan. This study suggests that RFLP of mtDNA can be used for the diversity analysis of pigeonpea and it gives some indications on the maternal lineage among the species. The variations in the mitochondrial DNA hybridization patterns also suggest the extensive rearrangement of the organelle genome among the Cajanus species.

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References

  1. Ahmad, F., P.M. Gur & A.E. Slinkard, 1992. Isozyme polymorphism and phylogenetic interpretations in the genus Cicer L. Theor Appl Genet 83: 620–627.

    Article  Google Scholar 

  2. Close, P.S., R.C. Shoemaker & P. Keim, 1989. Distribution of restriction site polymorphism within the chloroplast genome of the genus Glycine subgenus Soja. Theor Appl Genet 77: 768–776.

    CAS  Article  Google Scholar 

  3. Deu, M.P., Hamon, J. Chantereau, P. Dufour, A. D'hont & C. Lanaud, 1995. Mitochondrial DNA diversity in wild and cultivated sorghum. Genome 38: 635–645.

    PubMed  CAS  Google Scholar 

  4. Devos, K.M. & M.D. Gale, 1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84: 567–572.

    Article  Google Scholar 

  5. Dewey, R.E., C.S. Levings III & D.H. Timothy, 1985. Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79: 914–919.

    PubMed  CAS  Article  Google Scholar 

  6. Ennes R.A., W.T. Sinclair, X-S. Hu & A. Langdon, 1999. Using organelle markers to elucidate the history, ecology and evolution of plant populations. In: P.M. Hollingsworth, R.M. Bateman & R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 1–19. Taylor & Francis, London.

    Google Scholar 

  7. Feinburg, A.P. & B. Vogelstein, 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267.

    Google Scholar 

  8. Gawel, N.J., R.L. Jarret & A. Whittemore, 1992. Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa. Theor Appl Genet 84: 286–290.

    Article  Google Scholar 

  9. Gonzalez, J.M. & E. Ferrer, 1993. Random amplified polymorphic DNA analysis in Hordeum species. Genome 36: 1029–1031.

    CAS  PubMed  Google Scholar 

  10. Grabau, E.A., W.H. Davis, N.D. Phelps & B.G. Gengenbach, 1992. Classification of soybean cultivars based on mitochondrial restriction fragment length polymorphisms. Crop Sci 32: 271–274.

    CAS  Article  Google Scholar 

  11. Harris, S.A., 1999. RAPDs in sytematics - a useful methodology? In: P.M. Hollingsworth, R.M. Bateman and R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 211–228. Taylor & Francis, London.

    Google Scholar 

  12. Hershkovitz, M.A., E.A. Zimmer & W.J. Hahn, 1999, Ribosomal DNA sequences and angiosperm systematics. In: P.M. Hollingsworth, R.M. Bateman & R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 268–326. Taylor & Francis, London.

    Google Scholar 

  13. Hongtrakul, V., G.M. Huestis & S.J. Knap, 1997. Amplified fragment length polymorphisms as tool for DNA fingerprinting sunflower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 95: 400–407.

    CAS  Article  Google Scholar 

  14. Isaac, P.G., V.P. Jones & C.J. Leaver, 1985. The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male-sterile plants. EMBO J 4: 1617–1623.

    PubMed  CAS  Google Scholar 

  15. Jain, A., S. Bhatia, S.S. Banga, S. Prakash & M. Lakshmikumaran, 1994. Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor Appl Genet: 116–122.

    Google Scholar 

  16. Jarret, R.L., N. Gawel & A. Whittemore, 1992. Phylogenetic relationships of the sweet potato (Ipomoea batatas (L.) Lam.] J Am Soc Hort Sci 117: 633–637.

    Google Scholar 

  17. Jena, K.K. & G.C. Kochert, 1991. Restriction fragment length polymorphism analysis of CCDD genome species of the genus Oryza L. Plant Mol Biol 16: 831–839.

    PubMed  CAS  Article  Google Scholar 

  18. Krishna, T.G. & L.J. Reddy, 1982. Species affinities between Cajanus cajan and some Atylosia species based on esterase isozymes. Euphytica 31: 709–713.

    Article  Google Scholar 

  19. Ladizinsky, G. & A. Hamel, 1980. Seed protein profiles of pigeonpea (Cajanus cajan) and some Atylosia species. Euphytica 29: 313–317.

    CAS  Article  Google Scholar 

  20. Levinson, G. & G.A. Gutman, 1987. Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Molec Biol Evol 4: 203–221.

    PubMed  CAS  Google Scholar 

  21. Mackill, D.J., 1995. Classifying Japonica rice cultivars with RAPD markers. Crop Sci 35: 889–894.

    CAS  Article  Google Scholar 

  22. McGregor C.E., C.A. Lambert, M.M. Greyling, J.H. Louw & L. Warnich, 2000. A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113: 135–144.

    CAS  Article  Google Scholar 

  23. Miller, J.C. & S.D. Tanksley, 1990. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80: 437–448.

    CAS  Google Scholar 

  24. Moeykens, C.A., S.A. Mackenzie & R.C. Shoemaker, 1995. Mitochondrial genome diversity in soybean: repeats and rearrangements. Plant Mol Biol 29: 245–254.

    PubMed  CAS  Article  Google Scholar 

  25. Nadimpalli, R.G., R.L. Jarret, S.C. Phatak & G. Kochert, 1993. Phylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphisms. Genome 36: 216–223.

    PubMed  CAS  Google Scholar 

  26. Nei, M & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci (USA) 76: 5269–5273.

    CAS  Article  Google Scholar 

  27. Nene, Y.L. & V.K. Sheila, 1990. Pigeonpea: Geography and importance. In: Y.L. Nene, S.D. Hall & V.K. Sheila (Eds.), The Pigeonpea, pp. 1-15. C.A.B. International.

  28. Provan, J., N. Saranzo, N.J. Wison, J.W. McNicol, M. Margante & W. Powel, 1999. The use of uniparentally inherited simple sequence repeat markers in plant population studies and systematics. In: P.M. Hollingsworth, R.M. Bateman & R.J. Gornall (Eds.), Molecular Systematics and Plant Evolution, pp. 35–50. Taylor and Francis, London.

    Google Scholar 

  29. Pundir, R.P.S. & R.B. Singh, 1985. Cytogenetics of F1 hybrids between Cajanus and Atylosia species and its phylogenetic implications. Theor Appl Genet 71: 216–220.

    Google Scholar 

  30. Ratnaparkhe, M.B., V.S. Gupta, M.R. Ven Murthy & P.K. Ranjekar, 1995. Genetic fingerprinting of pigeonpea (Cajanus cajan (L.) Millsp.] and its wild relatives using RAPD markers. Theor Appl Genet 91: 893–898.

    CAS  Article  Google Scholar 

  31. Reddy, L.J. & D.N. De, 1983. Cyto-morphological studies in Cajanus cajan ?Atylosia lineata. Ind J Genet 43: 96–103.

    Google Scholar 

  32. Reddy, L.J., J.M. Green & D. Sharma, 1981. Genetics of Cajanus cajan (L.) Mills sp. ?Atylosia spp. In: Proceedings of the International workshop on Pigeonpeas, 15-19 Dec 1980. ICRISAT Center, Vol 2, pp. 39–50. Patancheru, AP, India.

    Google Scholar 

  33. Singh, A.K., S. Sivaramakrishnan, M.H. Mengesha & C.D. Ramaiah, 1991. Phylogenetic relations in section Arachis based on seed protein profile. Theor Appl Genet 82: 593–597.

    Google Scholar 

  34. Sivaramakrishnan, S., K. Seetha, A. Nageshwar Rao & Laxman Singh, 1997. RFLP analysis of Cytoplasmic male-sterile lines of pigeonpea [Cajanus cajan (L.) Millsp.] developed by interspecific crosses. Euphytica 93: 307–312.

    CAS  Article  Google Scholar 

  35. Smartt, J., 1990. Grain Legumes: Evolution and Genetic Resources, pp. 278–293. Cambridge University Press, Cambridge.

    Google Scholar 

  36. Svitashev, S., T. Bryngelsson, A. Vershinin, C. Pedersen, T. Sall & R. von Bothmer, 1994. Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences. Theor Appl Genet 89: 801–810.

    CAS  Article  Google Scholar 

  37. Tozuka, A., H. Fukushi, T. Hirata, M. Ohara, A. Kanazawa, T. Mikami, J. Abe & Y. Shimamoto, 1998. Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA. Theor Appl Genet 96: 170–176.

    CAS  Article  Google Scholar 

  38. van der Maesen, L.J.G., 1986. Cajanus D.C. and Atylosia W. & A. (Leguminosae). Agric. University of Wageningen Papers 85-4 (1985). Agricultural University. Wageningen, The Netherlands, 225 pp.

    Google Scholar 

  39. van der Maesen, L.J.G., 1990. Pigeonpea: origin, history, evolution, and taxonomy. In: Y.L. Nene, S.D. Hall & V.K. Sheila (Eds.), The Pigeonpea, pp. 15-46. C.A.B. International.

  40. Wang, G., R. Mahalingam & H.T. Knap, 1998. (C-A) and (GA) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr. Theor Appl Genet 96: 1086–1096.

    CAS  Article  Google Scholar 

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Sivaramakrishnan, S., Kannan, S. & Reddy, L. Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA. Euphytica 125, 21–28 (2002). https://doi.org/10.1023/A:1015759318497

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  • Cajanus cajan
  • diversity
  • mtDNA
  • RFLP