Abstract
Short-chain dehydrogenases/reductases (SDR) constitute a large protein family of NAD(P)(H)-dependent oxidoreductase. They are defined by distinct, common sequence motifs and show a wide range of substrate specialisms. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human SDR-type dehydrogenase/reductase gene named Dehydrogenase/reductase (SDR family) member 1 (DHRS1). The DHRS1 cDNA is 1411 base pair in length, encoding a 314-amino-acid polypeptide which has a SDR motif. Northern blot reveals two bands, of about 0.9 and 1.4 kb in size. These two forms are expressed in many tissues. The DHRS1 gene is localized on chromosome 14q21.3. It has 9 exons and spans 9.2 kb of the genomic DNA.
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Oppermann U, Filling C & Jörnvall H (2001) Chem-Biol. Interact. 130-132: 699–705.
Jörnvall H, Persson B, Krook M, Atrian S, Gonzalez-Duarte R, Jeffery J & Ghosh D (1995) Biochemistry 34: 6003–6013.
Benach J, Atrian S, Ladenstein R & Gonzalez-Duarte R (2001) Chem-Biol. Interact. 130-132: 405–415.
Rossmann MG, Liljas A, Brunden CI & Banaszak LJ (1975) In The Enzymes, 3rd Edition, Vol. 11, (Ed. by Boyer PD), pp. 61–102, Academic Press, New York.
Wierenga RK, Maeyer MCH & Hol WGJ (1985) Biochemistry 24: 1346–1357.
Holm L, Sander C & Murzin A (1994) Nat. Struct. Biol. 1: 146–147.
Cols N, Atrian S, Benach J, Ladenstein R & Gonzalez-Duarte R (1997) FEBS Lett. 413: 191–193.
Hülsmeyer M, Hecht HJ, Niefind K, Hofer B, Eltis LD, Timmis KH & Schomburg D (1998) Protein Sci. 7: 1286–1293.
Tanaka N, Nonaka T, Nakanishi M, Deyashiki Y, Hara A & Mitsui Y (1996) Structure 4: 33–45.
Andersson A, Jordan D, Schneider G & Lindqvist Y (1997) FEBS Lett. 400: 173–176.
Ghosh D, Erman M, Wawrzak Z, Duax WL & Pangborn W (1994) Structure 2: 973-980.
Ghosh D, Wawrzak Z, Weeks CM, Duax WL & Erman M (1994) Structure 2: 629–640.
Persson B, Krook M & Jörnvall H (1991) Eur. J. Biochem. 200: 537–543.
Peltoketo H, Luu-The V, Simard J & Adamski J (1999) J. Mol. Endocrinol. 23: 1–11.
Oppermann UCT, Persson B, Filling C & Jörnvall H (1997) Adv. Exp. Med. Biol. 414: 403-415.
Kozak M (1986) Cell 44: 283–292.
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Wu, Q., Xu, M., Cheng, C. et al. Molecular cloning and characterization of a novel Dehydrogenase/reductase (SDR family) member 1 genea from human fetal brain. Mol Biol Rep 28, 193–198 (2001). https://doi.org/10.1023/A:1015722001960
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DOI: https://doi.org/10.1023/A:1015722001960