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Characterisation of genetic variation between Vitis vinifera cultivars from central Chile using RAPD and Inter Simple Sequence Repeat markers

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Abstract

The origins and authenticity of many grape cultivars (Vitis vinifera) used for wine production around the world is unclear and the subject of some controversy. In this study, DNA fingerprints generated by Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat Polymerase Chain Reaction (ISSR-PCR) analyses were used to compare the four most widely planted V. vinifera cultivars in Chile (viz. Cabernet Sauvignon, Cabernet Franc, Merlot and Carmenere). Material obtained from France was used as an external reference. Both techniques were able to distinguish between the cultivars studied although the resolving power of ISSR profiles was higher than that of RAPDs, suggesting that the latter would be more suited for use on a wider range of cultivars. Surprisingly, however, variability was observed between clones of Merlot, the original Chilean clone and the representative clone from France. Furthermore, the high degree of divergence between the two sources (64% similarity) suggests that the French Merlot is not even a close relative of the stock in Chile. Interestingly, the latter was derived directly from the original French founder clones of the Merlot cultivar. No variation was found within the Chilean Merlot clone using either ISSR or RAPD analyses. These results indicate that French and Chilean vines grown for Merlot production represent different genotypes. The history of Merlot cultivation and the implications of these findings are explored.

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References

  • Bourquín, J.C., L. Otten & B. Walter, 1993. Restriction fragment length polymorphism and molecular taxonomy in Vitis vinifera. Theor Appl Genet 87: 157–162.

    Google Scholar 

  • Bowers, J., E. Bandnan & C. Meredith, 1993. DNA fingerprint characterization of some wine grape cultivars. Am J Enol Vitic 44: 266–274.

    CAS  Google Scholar 

  • Bowers, J., G. Dangi, R. Vignani & C. Meredith, 1996. Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39: 628–633.

    CAS  Google Scholar 

  • Bowers, J. & C. Meredith, 1997. The parentage of a classic wine grape, Cabernet sauvignon. Nature Genetics 16: 84–87.

    Article  PubMed  CAS  Google Scholar 

  • Büscher, N., E. Zyprian & R. Blaich, 1993. Identification of grapevine cultivars by DNA analysis: Pitfalls of random amplified polymorphic DNA techniques using 10mer primers. Vitis 32: 187–188.

    Google Scholar 

  • Büscher, N., E. Zyprian, O. Bachmann & R. Blaich, 1994. On the origin of the grapevine variety Müller-Thurgau as investigated by the inheritance of random amplified polymorphic DNA (RAPD). Vitis 33: 15–17.

    Google Scholar 

  • Charters, Y.M., A. Robertson, M.J. Wilkinson & G. Ramsay, 1996. PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5_-anchored simple sequence repeat (SSR) primers. Theor Appl Genet 92: 442–447.

    Article  CAS  Google Scholar 

  • Doyle, J. & J. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

    Google Scholar 

  • Gilmour, M., 1994. The BCCCA Ringtest on the RAPD Analysis of Cocoa. Proc Int Work Cocoa Breed Strat, Kuala Lumpur, pp. 135–138.

  • Gogorcena, Y., S. Arulsekar, A. Danderkar & D. Parfitt, 1993. Molecular markers for grape characterization. Vitis 32: 183–185.

    CAS  Google Scholar 

  • Grando, M., L. De Micheli, L. Biosetto & A. Scienza, 1995. RAPD markers in wild and cultivated Vitis vinifera. Vitis 34: 37–39.

    CAS  Google Scholar 

  • Halldén, C., M. Hansen, N. Nilsson, A. Hjerdin & T. Säll, 1996. Competition as a source of errors in RAPD analysis. Theor Appl Genet 93: 1185–1192.

    Article  Google Scholar 

  • Herrera, R. 1998. Study of genetic diversity in Vitis vinifera using RAPDs. In: S. Burns, S. Mantell, C. Tragardh & A. Viana (Eds.), Recent Advances in Biotechnology for Tree Conservation and Management, pp. 112–121. International Foundation for Science, Stockholm, Sweden.

    Google Scholar 

  • Jean-Jaques, I., A. Defontaine & J. Hallet, 1993. Characterization of Vitis vinifera L. cultivars by random amplified polymorphic DNA markers. Vitis 32: 189–190.

    CAS  Google Scholar 

  • Lanham, P. & R. Brennan, 1998. Characterization of the genetic resource of red currant (Ribes rubrum: Subg. Ribesia) using anchored microsatellite markers. Theor Appl Genet 96: 917–921.

    Article  CAS  Google Scholar 

  • Lodhi, M., N. Weeden & B. Reisch, 1997. Characterization of RAPD markers in Vitis. Vitis 36: 133–140.

    Google Scholar 

  • Micheli, M, R. Bova, E. Pascale & E. Dámbrosio, 1994. Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method. Nucleic Acids Res 22: 1921–1922.

    PubMed  CAS  Google Scholar 

  • Moreno, S., Y. Gogorcena & J. Ortiz, 1995. The use of RAPD markers for identification of cultivated grapevine (Vitis vinifera L.). Sci Hort 62: 237–243.

    Article  CAS  Google Scholar 

  • Moreno, S., J. Martin & J. Ortiz, 1998. Inter simple sequence repeats PCR for characterization of closely related grapevine germplasm. Euphytica 101: 117–125.

    Article  CAS  Google Scholar 

  • Mulcahy, D., M. Cresti, S. Sansavini, G. Douglas, H. Linskens, G. Bergamini-Mulcahy, R. Vignani & M. Pancaldi, 1993. The use of random amplified polymorphic DNAs to fingerprint apple genotypes. Sci Hort 54: 89–96.

    Article  CAS  Google Scholar 

  • Paul, H. 1996. Science, Vine and Wine in Modern France. Cambridge University Press, pp. 347.

  • Pooler, M. & R. Scorza, 1995. Aberrant transmission of RAPD markers in haploids, double haploids, and F1 hybrids of peach: observations and speculation on causes. Sci Hort 64: 233–241.

    Article  Google Scholar 

  • Steenkamp, J., Y. Wiid & P. Helden, 1994. Improved method for DNA extraction from Vitis vinifera. Am J Enol 45: 102–106.

    CAS  Google Scholar 

  • Striem, M., P. Spiegel-Roy, E. Ben-Hayyim, J. Beckmann & D. Gidoni, 1990. Genomic DNA fingerprinting of Vitis vinifera by the use of multiloci probes. Vitis 29: 223–227.

    Google Scholar 

  • This, P., C. Cuisset, J. Boursiquot, 1997. Development of stable RAPD markers for the identification of grapevine rootstocks and the analysis of genetic relationships. Am J Enol Vitic 48: 492–501.

    CAS  Google Scholar 

  • Tschammer, J. & E. Zyprian, 1994. Molecular characterization of grapevine cultivars of Riesling-type and of closely related Burgundies. Vitis 84: 835–838.

    Google Scholar 

  • Unwin, T. 1991. Wine and the Vine: An Historical Geography of Viticulture and the Wine Trade. Routledge Press, London, UK.

    Google Scholar 

  • Vignani, R., J.E. Bowers & C.P. Meredith, 1996. Microsatellite DNA polymorphism analysis of clones of Vitis vinifera 'sangiovese'. Sci Hort 65: 163–169

    Article  CAS  Google Scholar 

  • Walker, M. & J. Boursiquot, 1992. Amplelografic and isozyme data correcting the misnaming of grape rootstock S04 at the University of California, Davis. Am J Enol Vitic 43: 261–265.

    CAS  Google Scholar 

  • Weeden, J., B. Reisch & M. Martens, 1988. Genetic analysis of isozyme polymorphism in grape. J Am Soc Hort Sci 113: 735–769.

    Google Scholar 

  • Weising, K., H. Nybom, K. Wolff & W. Meyer, 1995. DNA Fingerprinting in Plants and Fungi. CRC, Press, Inc., Boca Raton, USA.

    Google Scholar 

  • Ye, G., G. Soylemezoglu, N. Weeden, W. Lamboy, R. Pool & B. Reisch, 1998. Analysis of the relationship between grapevine cultivars, sports and clones via DNA fingerprinting. Vitis 37: 33–38.

    Google Scholar 

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Authors and Affiliations

  1. Instituto Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile

    R. Herrera & V. Cares

  2. Department of Agricultural Botany, School of Plant Sciences, The University of Reading, P.O. Box 221, Reading, RG6 6AS, U.K

    M.J. Wilkinson & P.D.S. Caligari

Authors
  1. R. Herrera
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  2. V. Cares
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  3. M.J. Wilkinson
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  4. P.D.S. Caligari
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Herrera, R., Cares, V., Wilkinson, M. et al. Characterisation of genetic variation between Vitis vinifera cultivars from central Chile using RAPD and Inter Simple Sequence Repeat markers. Euphytica 124, 139–145 (2002). https://doi.org/10.1023/A:1015693721532

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