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Hairy root cultures of Anethum graveolens (dill): establishment, growth, time-course study of their essential oil and its comparison with parent plant oils

Abstract

Transformed root cultures of Anethum graveolens were induced by inoculation of aseptically grown seedlings with Agrobacterium rhizogenes carrying plasmid pRi 1855. The main component of the essential oils from the fruits and from the roots of the parent plant was carvone, whereas α-phellandrene and apiole were dominant in the oil from, respectively, the aerial parts and the hairy roots. The essential oils from the fruits, aerial parts and roots of the parent plant were at 2%, 0.3% and 0.06% (v/w), respectively, but only 0.02% (v/w) in the hairy root cultures. Growth of the hairy root cultures reached 600 mg dry wt/50 ml medium after 50 days. The essential oil composition did not change significantly during their growth.

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References

  • Banthorpe DV, Branch SA, Njar VCO, Osborne MG, Watson DG (1986) Ability of plant callus cultures to synthesize and accumulate lower terpenoids. Phytochemistry 25: 629–636.

    Google Scholar 

  • Bernart MW, Cardellina JH, Balaschak MS, Alexander MR, Shoemaker RH, Boyd MR (1996) Cytotoxic falcarinol oxylipins from Dendropanax arboreus. J. Nat. Prod. 59: 748–753.

    Google Scholar 

  • Bohlmann F, Jakupovic J, Ahmed M, Grenz M, Suding H, Robinson H, King RM (1981) Germacranolides and diterpenes from Viguiera species. Phytochemistry 29: 113–116.

    Google Scholar 

  • Cunsolo F, Ruberto G, Amico V, Piattelli M (1993) Bioactive metabolites from Sicilian marine fennel, Crithmum maritimum. J. Nat. Prod. 56: 1598–1600.

    Google Scholar 

  • Everitt ZM, Lockwood GB (1992) Biotransformation of geraniol by agitating and immobilised cultures of Anethum graveolens. Fitoterapia 63: 534–536.

    Google Scholar 

  • Everitt ZM, Lockwood GB (1995) Anethum graveolens L. (Dill). In vitro culture and metabolism of volatile constituents. In: Bajaj YPS, ed. Biotechnology in Agriculture and Forestry, Vol. 33, Medicinal and Aromatic Plants III. Berlin: Springer-Verlag, pp. 21–34.

    Google Scholar 

  • Feijão RD'O (1979) Medicina Pelas Plantas, 7th ed. Lisbon: Livraria Progresso Editora, p. 138.

    Google Scholar 

  • Flores HE, Vivanco JM, Loyola-Vargas VM (1999) 'Radicle' biochemistry: the biology of root-specific metabolism. Trends Plant Sci. 4: 220–226.

    Google Scholar 

  • Font Quer P (1981) Plantas Medicinales, El Dioscórides Renovado. Barcelona: Editorial Labor S.A., pp. 500–501.

    Google Scholar 

  • Gafner F, Reynolds GW, Rodriguez E (1989) The diacetylene 11,12-dehydrofalcarinol from Hedera helix. Phytochemistry 28: 1256–1257.

    Google Scholar 

  • Lawless J (1995) The Illustrated Encyclopedia of Essential Oils. Shaftesbury, Dorset: Element, p. 83.

    Google Scholar 

  • Lawrence BM (1985/6) Progress in essential oils. Perfum. Flavor. 10: 29.

    Google Scholar 

  • Lourenço PML, Figueiredo AC, Oliveira MM, Barroso JG, Pedro LG, Deans SG, Scheffer JJC (1999) Essential oils from hairy root cultures and from plant roots of Achillea millefolium. Phytochemistry 51: 637–642.

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15: 473–497.

    Google Scholar 

  • Santos PM, Figueiredo AC, Oliveira MM, Barroso JG, Pedro LG, Deans SG, Younus AKM, Scheffer JJC (1998) Essential oils from hairy root cultures and from fruits and roots of Pimpinella anisum. Phytochemistry 48: 455–460.

    Google Scholar 

  • Vasconcellos JC (1949) Plantas Medicinales e Aromáticas. Lisbon: Direcção Geral dos Serviços Agrícolas, pp. 122–123.

    Google Scholar 

  • Zheng G, Lu W, Aisa HA, Cai J (1999) Absolute configuration of falcarinol, a potent antitumor agent commonly occurring in plants. Tetrahedron Lett. 40: 2181–2182.

    Google Scholar 

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Santos, P.A., Figueiredo, A.C., Lourenço, P.M. et al. Hairy root cultures of Anethum graveolens (dill): establishment, growth, time-course study of their essential oil and its comparison with parent plant oils. Biotechnology Letters 24, 1031–1036 (2002). https://doi.org/10.1023/A:1015653701265

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  • DOI: https://doi.org/10.1023/A:1015653701265

  • Agrobacterium rhizogenes
  • Anethum graveolens
  • Apiaceae
  • essential oil
  • hairy root cultures