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Hairy root cultures of Anethum graveolens (dill): establishment, growth, time-course study of their essential oil and its comparison with parent plant oils

Biotechnology Letters volume 24, pages 1031–1036 (2002)Cite this article

Abstract

Transformed root cultures of Anethum graveolens were induced by inoculation of aseptically grown seedlings with Agrobacterium rhizogenes carrying plasmid pRi 1855. The main component of the essential oils from the fruits and from the roots of the parent plant was carvone, whereas α-phellandrene and apiole were dominant in the oil from, respectively, the aerial parts and the hairy roots. The essential oils from the fruits, aerial parts and roots of the parent plant were at 2%, 0.3% and 0.06% (v/w), respectively, but only 0.02% (v/w) in the hairy root cultures. Growth of the hairy root cultures reached 600 mg dry wt/50 ml medium after 50 days. The essential oil composition did not change significantly during their growth.

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Authors and Affiliations

  1. Centro de Biotecnologia Vegetal, Faculdade de Cîencias de Lisboa, Departamento de Biologia Vegetal, Bloco C2, Campo Grande, 1749-016, Lisbon, Portugal

    Pedro A.G. Santos, A. Cristina Figueiredo, Pedro M.L. Lourenço, José G. Barroso & Luis G. Pedro

  2. ITQB/IBET, Quinta do Marqûes, Aptd. 12, 2781-901, Oeiras, Portugal

    M. Margarida Oliveira

  3. Setor de Quimica de Produtos Naturais, LCQUI/CCT, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28015-620, Campo dos Goytacazes, Rio de Janeiro, Brasil

    Jan Schripsema

  4. Department of Food Science & Technology, SAC, Auchincruive, Ayr, KA6 5HW, Scotland, UK

    Stanley G. Deans

  5. Division of Pharmacognosy, LACDR, Leiden University, Gorlaeus Laboratories, PO Box 9502, 2300 RA, Leiden, The Netherlands

    Johannes J.C. Scheffer

Authors
  1. Pedro A.G. Santos
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  2. A. Cristina Figueiredo
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  3. Pedro M.L. Lourenço
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  4. José G. Barroso
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  5. Luis G. Pedro
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  6. M. Margarida Oliveira
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  7. Jan Schripsema
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  8. Stanley G. Deans
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  9. Johannes J.C. Scheffer
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Santos, P.A., Figueiredo, A.C., Lourenço, P.M. et al. Hairy root cultures of Anethum graveolens (dill): establishment, growth, time-course study of their essential oil and its comparison with parent plant oils. Biotechnology Letters 24, 1031–1036 (2002). https://doi.org/10.1023/A:1015653701265

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  • DOI: https://doi.org/10.1023/A:1015653701265

  • Agrobacterium rhizogenes
  • Anethum graveolens
  • Apiaceae
  • essential oil
  • hairy root cultures