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A two-enzyme system for the transformation of unsaturated oils to 9(S)-hydroperoxy fatty acids

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Abstract

A two-enzyme system involving a lipase from a Pseudomonassp. and an extract of potato tubers containing lipoxygenase was used to convert triacylglycerols to 9-hydroperoxy fatty acids. Highest yields (up to 25%) were obtained with 1 to 20 g l−1 trilinolein as substrate after 5 h under O2 at pH 6 and 25 °C. The product structure was confirmed by 1H NMR, MS and IR.

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Authors and Affiliations

  1. Biological Engineering Unit, National Institute of Applied Science and Technology (INSAT), Tunis, Tunisia, 71 704 329

    Mohamed Gargouri

  2. Laboratoire de Génie Protéique et Cellulaire, Université de La Rochelle, France

    Marie Dominique Legoy

Authors
  1. Mohamed Gargouri
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  2. Marie Dominique Legoy
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Gargouri, M., Dominique Legoy, M. A two-enzyme system for the transformation of unsaturated oils to 9(S)-hydroperoxy fatty acids. Biotechnology Letters 24, 915–918 (2002). https://doi.org/10.1023/A:1015588008300

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  • DOI: https://doi.org/10.1023/A:1015588008300

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