Clinical & Experimental Metastasis

, Volume 19, Issue 3, pp 259–264 | Cite as

IFNγ and TNFα account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages

  • Lido Calorini
  • Francesca Bianchini
  • Antonella Mannini
  • Gabriele Mugnai
  • Manuela Balzi
  • Aldo Becciolini
  • Salvatore Ruggieri

Abstract

In the present study, we found that murine peritoneal macrophages elicited by BCG or Listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of MHC class I antigens in B16 melanoma cells. A similar activity has previously been found in media conditioned by Corynebacterium parvum-elicited macrophages. Analysis by gel filtration chromatography of media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages revealed that the material responsible for the pro-clonogenic activity concentrated in chromatographic fractions corresponding to molecular weights (25 to 52 kDa) which are characteristic of certain cytokines. Thus, we challenged the various macrophage-conditioned media with polyclonal antibodies against IFNγand TNFα, and found that the macrophage pro-clonogenic activity was completely abolished in the presence of anti-IFNγantibodies, but only partially inhibited by anti-TNFαantibodies. This finding suggests a cooperative participation of the two cytokines to the pro-clonogenic activity of the media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages.

F10-M3 cells Corynebacterium parvum- BCG- or Listeria monocytogenes-elicited macrophages MHC class I antigens lung colonization macrophage pro-clonogenic acitivity IFNγ TNFα 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cecconi O, Calorini L, Mannini A et al. Enhancement of lung-colonizing potential of murine tumor cell lines co-cultivated with activated macrophages. Clin Exp Metastasis 1997; 15: 94–101.PubMedCrossRefGoogle Scholar
  2. 2.
    Calorini L, Mannini A, Bianchini F et al. Biological properties associated with the enhanced lung-colonizing potential in a B16 murine melanoma line grown in a medium conditioned by syngeneic Corynebacterium parvum-elicited macrophages. Clin Exp Metastasis 1999; 17: 889–95.PubMedCrossRefGoogle Scholar
  3. 3.
    Gattoni-Celli S, Calorini L, Simile MM et al. Modulation by MHC Class I antigens of the biology of melanoma cells. Non-immunological mechanisms. Melanoma Res 1993; 3: 285–9.PubMedGoogle Scholar
  4. 4.
    Chen TR. In situ detection of Myeoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res 1977; 104: 255–62.PubMedCrossRefGoogle Scholar
  5. 5.
    Havell EA, Spitalny GL. Two molecular weight species of murine gamma interferon. Virology 1983; 129: 508–13.PubMedCrossRefGoogle Scholar
  6. 6.
    Wingfield P, Pain RH, Craig S. Tumor necrosis factor is a compact trimer. FEBS Lett 1987; 211: 179–84.PubMedCrossRefGoogle Scholar
  7. 7.
    Billiau A. Interferon-γ: Biology and role in pathogenesis. Adv Immunol 1996; 62: 61–130.PubMedCrossRefGoogle Scholar
  8. 8.
    Puddu P, Fantuzzi L, Borghi P et al. IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages. J. Immunol 1997; 159: 3490–7.PubMedGoogle Scholar
  9. 9.
    Guillemard E, Geniteau-Legendre M, Kergot R et al. Simultaneous production of IFN-gamma, IFN-alpha/beta and nitric oxide in peritoneal macrophages from TDM-treated mice. J Biol Regul Homeost Agents 1998; 12: 106–11.PubMedGoogle Scholar
  10. 10.
    Taniguchi K, Petersson M, Hoglund P et al. Interferon γ induces lung colonization by intravenously inoculated B16 melanoma cells in parallel with enhanced expression of class I major histocompatibility complex antigens. Proc Natl Acad Sci USA 1987; 84: 3405–9.PubMedCrossRefGoogle Scholar
  11. 11.
    McMillan TJ, Rao J, Everett CA et al. Interferon-induced alterations in metastatic capacity, class-I antigen expression and natural killer cell sensitivity of melanoma cells. Int J Cancer 1987; 40: 659–63.PubMedGoogle Scholar
  12. 12.
    Zoller M, Strubel A, Hammerling G et al. Interferon-gamma treatment of B16 melanoma cells: Opposing effects for non-adaptive and adaptive immune defense and its reflection by metastatic spread. Int J Cancer 1988; 41: 256–66.PubMedGoogle Scholar
  13. 13.
    Ramani P, Balkwill FR. Enhanced metastases of a mouse carcinoma after in vitro treatment with murine interferon gamma. Int J Cancer 1987; 40: 830–4.PubMedGoogle Scholar
  14. 14.
    Kelly SA, Gschmeissner S, East N et al. Enhancement of metastatic potential by γ-interferon. Cancer Res 1991; 51: 4020–7.PubMedGoogle Scholar
  15. 15.
    Lollini PL, De Giovanni C, Nicoletti G et al. Enhancement of experimental metastatic ability by tumor necrosis factor-alpha alone or in combination with interferon-gamma. Clin Exp Metastasis 1990; 8: 215–24.PubMedCrossRefGoogle Scholar
  16. 16.
    Piontek G, Taniguchi K, Ljunggren H et al. YAC-1 MHC class I variants reveal association between decreased NK sensitivity and increased H-2 expression after interferon treatment or in vivo passage. J Immunol 1985; 135: 4281–8.PubMedGoogle Scholar
  17. 17.
    Taniguchi K, Karre K, Klein G. Lung colonization and metastasis by disseminated B16 melanoma cells: H-2 associated control at the level of the host and the tumor cells. Int J Cancer 1985; 36: 503–10.PubMedGoogle Scholar
  18. 18.
    Kawano Y-I, Taniguchi K, Toshitani A et al. Synergistic defense system by cooperative natural effectors against metastasis of B16 melanoma cells in H-2-associated control: Different behaviour of H-2+ and H-2 cells in metastatic process. J Immunol 1986; 136: 4729–34.PubMedGoogle Scholar
  19. 19.
    Carrel S, Hartmann F, Salvi S et al. Expression of type A and B tumor necrosis factor (TNF) receptors on melanoma cells can be regulated by dbe-AMP and IFNγ. Int J Cancer 1995; 62: 76–83.PubMedGoogle Scholar
  20. 20.
    Saiki I, Maeda H, Murata J et al. Antimetastatic effect of endogenous tumor necrosis factor induced by the treatment of recombinant interferon gamma followed by an analogue (GLA-60) to synthetic lipid A subunit. Cancer Immunol Immunother 1989; 30: 151–157.PubMedCrossRefGoogle Scholar
  21. 21.
    Schultz RM, Altom MG. Protective activity of recombinant murine tumor necrosis factor-alpha and interferon-gamma against experimental murine lung carcinoma metastases. J Interferon Res 1990; 10: 229–36.PubMedGoogle Scholar
  22. 22.
    Ruegg C, Yilmaz A, Bieler G et al. Evidence for the involvement of endothelial cell integrin alphaVbeta3 in the disruption of the tumor vasculature induced by TNF and IFN-gamma. Nat Med 1998; 4: 408–14.PubMedCrossRefGoogle Scholar
  23. 23.
    van Moorselaar RJA, Hendriks BT, van Stratum P et al. Synergistic antitumor effects of rat γ-interferon and human tumor necrosis factor α against androgen-dependent and-independent rat prostatic tumors. Cancer Res 1991; 51: 2329–34.PubMedGoogle Scholar
  24. 24.
    Tracey KJ, Cerami A. Tumor necrosis factor: A pleiotropic cytokine and therapeutic target. Annu Rev Med 1994; 45: 491–503.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Lido Calorini
    • 1
  • Francesca Bianchini
    • 1
  • Antonella Mannini
    • 1
  • Gabriele Mugnai
    • 1
  • Manuela Balzi
    • 2
  • Aldo Becciolini
    • 2
  • Salvatore Ruggieri
    • 1
  1. 1.Department of Experimental Pathology and Oncology, Radiation Biology LaboratoryUniversity of FlorenceItaly
  2. 2.Department of Clinical Physiopathology, Radiation Biology LaboratoryUniversity of FlorenceItaly

Personalised recommendations